Basic research Co-culture with Schwann cells is an effective way for adipose-derived stem cells neural transdifferentiation

Abstract

IntroductionAdipose-derived stem cells (ADSCs) could accomplish neural transdifferentiation with the presence of certain growth factors in vitro. It has been proved that bone marrow stromal cells (BMSCs) can realize neural transdifferentiation only by being co-cultured with Schwann cells (SCs), and in our former studies we have confirmed that ADSCs could do so too. This paper aims to investigate whether the neural induction efficiency of co-culture is as high as that of other strategies using chemicals or chemicals combined with some growth factors.Material and methodsWe isolated and multiplied ADSCs from adult Sprague-Dawley rats, and SCs from sciatic nerves of 1-to-2-day-old Sprague-Dawley rat pups, then induced ADSCs neural transdifferentiation through 2% dimethyl sulphoxide (DMSO) and DMSO combined with growth factors. Meanwhile we co-cultured ADSCs and SCs in Transwell culture dishes without intercellular contacts. Immunostaining and RT-PCR were adopted to investigate the neural transdifferentiation of ADSCs. Then we compared the expression differences for genes S100, nestin and GFAP of the above three protocols by real-time PCR.ResultsBoth immunostaining and RT-PCR proved that ADSCs could accomplish neural transdifferentiation through each of the above three protocols. And real-time PCR further shows that the gene expression relative quantities for the above three genes are not statistically different between co-culture and induction through DMSO combined with growth factors (p > 0.05), but both of them are statistically different from induction only by DMSO (p < 0.05).ConclusionsCo-culturing ADSCs and SCs may be a simple, effective and practical way for ADSCs neural transdifferentiation.

Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application

“…Unfortunately, autologous SCs isolation is an invasive procedure and it is difficult to get sufficient number of SCs for clinical application. However, recent studies demonstrated that ADSCs seems to be one ideal cell source for transdifferentiation into SCs [40]. In our previous study, using a standard protocol, ADSCs were induced into SCs [22].…”

Mesenchymal Stem Cells: Current Clinical Applications and Therapeutic Potential in Liver Diseases

“…These unique biological properties of MSCs highlight great potential in several applications such as regenerative medicine, tissue engineering and cell-based therapy. Here, this review focuses on an overview of MSCs biological International Society for Cellular Therapy (ISCT) has proposed minimal criteria for defining MSCs in 2006 [20]. The characteristics of MSCs can be described at least 3 definitions as followed.…”

Transplantation of Adipose-Derived Stem Cells in Stroke

“…Despite the relatively small number of ADSC to explain the overall functional recovery in the reported studies, their presence signifies "transdifferentitation" as a possible mechanism underlying the positive therapeutic impact (Gutierrez-Fernandez et al 2013a). Indeed, the capacity of neural differentiation for ADSC has been extensively investigated (Cardozo et al 2010;Kompisch et al 2010;Liao et al 2010;Qian et al 2010;Abdanipour et al 2011;Yu et al 2011;Ahmadi et al 2012). It has also been reported that, compared with bone marrow-derived mesenchymal stem cells, ADSC have superior neurogenic potential (Kang et al 2004).…”