Basic Techniques and Limitations in Establishing Cell Culture: a Mini Review

Swain, P.

doi:10.14737/journal.aavs/2014/2.4s.1.10

Cited by 9 publications

(16 citation statements)

References 110 publications

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“…When 5% trypsin solution was tested, obtained cells were dead [72]. Until then, cultures were obtained from the tissue explants, and use of trypsin facilitated procedure of obtaining homogenous cell strains [73].…”

Section: Evaluation Of Cell Culture Techniques and Establishing Princmentioning

confidence: 99%

“…Fetal bovine serum (FBS) and newborn calf serum (NCS) are most common. For more specific cultures human, horse or rabbit sera are used [52,73] Antibiotics and antimycotic solutions Antibiotics and antifungal with laminar flow hoods reduced the frequency of contamination. In cell cultures most often penicillin streptomycin solutions are used.…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

“…In cell cultures most often penicillin streptomycin solutions are used. As the antimycotic agents the kanamycin or amphotericin B are applied [52,73,79] Growth factors and hormones Hormones and growth factors are used especially in serum-free media. Those factors are ensured cellular growth, division, and differentiation.…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

“…The most popular are fibroblastic growth factor (FGF), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF) or platelet derived growth factor (PDGF). In the group of hormones the most common are hydrocortisol and insulin [52,73,79] New Insights into Cell Culture Technology…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

“…The pH level can be checked by presence of phenol red in culture medium [52,73,79] Oxygen The oxygen, as a part of the gas phase is required for adequate cell physiology, function, and differentiation. The oxygen requirements are depend on the type of cells.…”

Section: Physico-chemical Properties Of Cultures In Vitromentioning

confidence: 99%

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History of Cell Culture

Jędrzejczak-Silicka¹

2017

New Insights Into Cell Culture Technology

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From the ancient Romans, through the Middle Ages, to the late of the nineteenth century, the Aristotelian doctrine of spontaneous generation was one of the most basic laws. Even the invention of the microscope and investigations of Leeuwenhoek and Hook did not disprove the Aritostelian doctrine. Finally, in the eighteenth century, the spontaneous generation doctrine was laid by Louis Pasteur. Moreover, in the first decade of the eighteenth century, nucleus was observed in plant and animal tissues, and Virchow and other scientists presented the view that cells are formed via scission of preexisting cells. In the first decade of the twentieth century, Ross Harrison developed the first techniques of cell culture in vitro, and Burrows and Carrel improved Harrison's cell cultures. In mid-twentieth century, the basic principles for plant and animal cell cultures in vitro were developed, and human diploid cell lines were established. On the basis of knowledge about the cell cycle and gene expression regulation, the first therapeutic proteins were produced using mammalian cell cultures. The end of twentieth century and early twenty-first century brought the progress in 3-D cell culture technology and created the possibility of the tissue engineering and the regenerative medicine development.

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Section: Evaluation Of Cell Culture Techniques and Establishing Princmentioning

confidence: 99%

Section: Cell Culture Conditionsmentioning

confidence: 99%

Section: Cell Culture Conditionsmentioning

confidence: 99%

Section: Cell Culture Conditionsmentioning

confidence: 99%

Section: Physico-chemical Properties Of Cultures In Vitromentioning

confidence: 99%

See 3 more Smart Citations

History of Cell Culture

Jędrzejczak-Silicka¹

2017

New Insights Into Cell Culture Technology

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Microfluidic Reconstitution of Tumor Microenvironment for Nanomedical Applications

Kim

et al. 2021

Adv Healthcare Materials

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Nanoparticles have an extensive range of diagnostic and therapeutic applications in cancer treatment. However, their current clinical translation is slow, mainly due to the failure to develop preclinical evaluation techniques that can draw similar conclusions to clinical outcomes by adequately mimicking nanoparticle behavior in complicated tumor microenvironments (TMEs). Microfluidic methods offer significant advantages over conventional in vitro methods to resolve these challenges by recapitulating physiological cues of the TME such as the extracellular matrix, shear stress, interstitial flow, soluble factors, oxygen, and nutrient gradients. The methods are capable of de‐coupling microenvironmental features, spatiotemporal controlling of experimental sequences, and high throughput readouts in situ. This progress report highlights the recent achievements of microfluidic models to reconstitute the physiological microenvironment, especially for nanomedical tools for cancer treatment.

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Application of standard cell cultures and 3D in vitro tissue models as an effective tool in drug design and development

Amelian

Wasilewska

Megı́as

et al. 2017

Pharmacological Reports

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Cell culture systems are essential tools used in a wide range of biomedical and clinical studies. Two dimensional cell culture models (2D) provide basic information on cytotoxicity, penetration and accumulation of drugs in cells and they are of outmost importance when selecting new compounds of the desired biopharmaceutical properties as candidates for novel drugs. The improvement over 2D growing cells are three dimensional (3D) tissue models that mimic in vivo conditions and the functions of living tissue more accurately. These models reduce the cost of drug development, enable more efficient drug screening, minimise failure rate in medicine discovery and eliminate animal use during experiments. The article provides an overview of 2D cell cultures and 3D tissue models - their properties, basic procedures, conditions of culturing and applications.

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Basic Techniques and Limitations in Establishing Cell Culture: a Mini Review

Cited by 9 publications

References 110 publications

History of Cell Culture

History of Cell Culture

Microfluidic Reconstitution of Tumor Microenvironment for Nanomedical Applications

Application of standard cell cultures and 3D in vitro tissue models as an effective tool in drug design and development

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