Basophilic Chromidia and Mitochondria in Normal and in Tumor Tissue

Abstract

of the light chromidia (microsomes), which were spun down after two hours at 18,000 x g with Servall SS-1 centrifuge from samples diluted with sufficient water to make the sucrose isotonic. If the initial centrifugal force was below 600 x g, a heavy contamination

An Electron Microscopic Correlation of Structure with Function in the Isolated Perfused Cow Adrenal, Preliminary Observations

“…Comparison of the ACTI-Itreated and control glands reveals a greater density and number of cytoplasmic granules in the former (P). This finding is consistent with the increase in RNA of the microsomal fraction observed in vivo after several hours administration of ACTH (5).…”

The Action of Corticotropin (Acth) on Nucleic Acids and Subcellular Elements of the Adrenal Cortex

“…It was not regarded as necessary to separate the so called "poorly sedlmented" or "fluffy" layer (4,5). This fraction, which stains with pyronine Y in distinction to mitochondria (6) was intentionally in- Cluded among other basophllic granules which sedimented after 90 minutes at the top speed of the Servall centrifuge. The centrifugation procedure used is illustrated by the diagram shown in Table I.…”

The Intracellular Metabolism of 3:4 Benzpyrene: Metabolism in the Kidneys and Skin of Rats and Mice

“…The answer to this problem may lie in the fact that the present experiments have been concerned with skin from very young animals. Fiala, Sproul and Fiala (1955) stated that day-old mice painted with benzpyrene responded with tumour formation, but there appear to be no records of similar experiments with young rats.…”

“…It has already been shown by Fiala, Sproul and Fiala (1955) that benzpyrene penetrates to and is bound in nuclei, mitochondria, microsomes and supernatant of mouse skin cells. In the present experiments the hydrocarbon has been detected in all four fractions of rat skin cells 21, 6 and 22 hours after painting with the hydrocarbon.…”

“…One is composed of unbroken cells and the particulate components of the disrupted cells whilst the other is a clear supernatant fraction. However, Fiala, Sproul and Fiala (1955) showed that adequate separations can be achieved if the skin of very young mice is used. This finding has been confirmed and also extended to cover skin from day-old rats.…”