Bazı Birbirine Benzer Elma (&lt;i&gt;Malus domestica&lt;/i&gt; L.) Genotiplerinde Pomolojik ve Moleküler Yöntemlerle Genetik Akrabalık Derecelerinin Tespiti

DALER, Selda; Aşkın, Mehmet Atilla; Karakurt, Yaşar

doi:10.19113/sdufbed.50851

Cited by 4 publications

(1 citation statement)

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“…Markers such as RAPD, SSR, nrDNA ITS, cpDNA matK, atpB, and atpB-rbcL were used in these studies (Savolainen et al 1995, Hokanson et al 1998, Robinson et al 2001, Kaya et al 2015, Mahmood et al 2016, Daler et al 2017. Daler et al (2017) specified the relationship degree of genotypes using RAPD molecular technique on 6 apple genotypes obtained from Isparta/Eğirdir Fruit Research Institute Directorate's collection. Kaya et al (2015) carried out molecular analysis of Apple genotypes collected from Van province, through using RAPD markers.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization based on chloroplast (trnl-f) DNA sequence of the apple genotypes in Ardahan/Turkey

et al. 2019

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Genetic diversity among Turkish apple genotypes in Ardahan province was conducted based on cpDNA trnL-F sequences. Apple genotypes were plotted on a phylogenetic tree where Pyrus x bretschneideri was used as the outgroup. The plant samples were collected from different locations and genomic DNA was isolated from healthy and green leaves. For sequence in trnL-F region trnLe and trnFf primers were used. Later obtained DNA sequences were edited using the BioEdit and FinchTV. Sequencing data were analyzed using MEGA 6.0 software. Neighbor joining and bootstrap trees were constructed in order to verify the relationships among the apple genotypes. Phylogenetic tree consisted of two clades. The divergence values of trnL-F sequences differed between 0.000 and 0.005. Average nucleotide composition was 38.3 T, 14.9 C, 31.9 A and 14.9% G. The phylogenetic tree constructed based on trnL-F region sequences was nearly parallel to prior phylogenetic studies on apple genotypes.

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Section: Resultsmentioning

confidence: 99%

Molecular characterization based on chloroplast (trnl-f) DNA sequence of the apple genotypes in Ardahan/Turkey

et al. 2019

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Genetic Diversity Analysis of Aydın/Turkey Dittrichia viscosa (L). Greuter (Asteraceae) Populations Using RAPD Markers

Sevindik¹,

Uzun²,

Paksoy³

2019

Türk Tarım Ve Doğa Bilimleri Dergisi

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In this study, we performed a genetic diversity using RAPD markers for some Dittrichia viscosa populations grown in the Aydın region of Turkey. Total genomic DNA isolation from the leaves of Dittrichia viscosa was performed using commercial kit. eight RAPD primers were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all DNA samples and primers with ability of scoreing band. PCR products were run in agarose gel and visualized under UV light. They scored as there were bands (1) and no bands (0) at all gel images and their files were created. A total of 50 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* 4.0b10 analysis program. According to the PAUP data, the closest genetic distance was determined 0.20000 and between Central and İncirliova populations, the most distant genetic distance were determined 0.36842 between İncirliova and Koçarlı populations. In the phylogenetic analysis has been obtained using UPGMA algorithms and phylogenetic tree consist of two clades. The results also propose that RAPD markers are useful tools for indicating genetic relationships among Dittrichia viscosa populations.

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Kırgız Milli Ala Arça Parka Ait Yerli Çeşitler Araştırma İstasyonunda Yetişen Albenisi Yüksek Bazı Elma (Malus x domestica Borkh.) Çeşitlerinin Bazı Meyve Özellikleri

Turgunbaeva,

Aygün

2024

International Journal of Life Sciences and Biotechnology

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Bu araştırma, Kırgızistandaki Bişkek şehri içerisinde bulunan farklı yerlereden daha önceden toplanarak kurulmuş yerel elma çeşitlerine ait koleksiyon bahçesinde yetiştirilen bazı elma çeşitlerinin meyve özelliklerinin belirlenmesi amacıyla yürütülmüştür. Çalışmada toplam 18 adet yerel elma çeşidi incelenmiştir. Elma çeşitlerinde meyve ağırlığı 42.28- 261.25 g , meyve boyu 25.39-73.31 mm, meyve eni 30.58-94.19 mm, meyve kalınlığı 31.41-91.36 mm, sap çukuru derinliği 5.22-17.58 mm, sap çukuru eni 9.88-26.42 mm, sap çukuru genişliği 8.59-31.80 mm, sap uzunluğu 13.32-32.21 mm, sap kalınlığı 1.16 -9.47 mm, meyve çiçek çukuru derinliği 5.75-28.69 mm, meyve çiçek çukuru genişliği 8.73-22.61 mm, meyve çiçek çukuru eni 4.14-17.46 mm, çekirdek sayısı 3.22-8.60 adet, meyve eti sertliği 2.51-11.35 kg/cm², suda çözünebilir kuru madde miktarı %3.48-16.75, pH 3.02 ile 7.40, titre edilebilir asitlik miktarı 27.71-3.10 aralığında bulunmuştur. Bu sonuçlar bölgede farklı lokasyonlardan daha önce toplanan elmalarda özellik bakımından farklılıklar olduğunu ortaya koymasının yanında bu çeşitlerin piyasada talep edilebileceği fikrini doğurmuştur. Diğer yandan bu genetik kaynakları oluşturan bahçenin yaşlanması ve bakımsız olması yüzünden bu çeşitler yok olma tehlikesi altındadır.

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Bazı Birbirine Benzer Elma (<i>Malus domestica</i> L.) Genotiplerinde Pomolojik ve Moleküler Yöntemlerle Genetik Akrabalık Derecelerinin Tespiti

Cited by 4 publications

References 2 publications

Molecular characterization based on chloroplast (trnl-f) DNA sequence of the apple genotypes in Ardahan/Turkey

Molecular characterization based on chloroplast (trnl-f) DNA sequence of the apple genotypes in Ardahan/Turkey

Genetic Diversity Analysis of Aydın/Turkey Dittrichia viscosa (L). Greuter (Asteraceae) Populations Using RAPD Markers

Kırgız Milli Ala Arça Parka Ait Yerli Çeşitler Araştırma İstasyonunda Yetişen Albenisi Yüksek Bazı Elma (Malus x domestica Borkh.) Çeşitlerinin Bazı Meyve Özellikleri

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