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Abstract

ABSTRACT:Coleus forskohlii is an Indian medicinal plant it treat various diseases of cardiovascular, respiratory, gastrointestinal, and central nervous systems. The purpose of this study was to investigate the anticancer activity of an ethanol extract of Coleus forskohlii against gastric cancer in AGS cancer cell lines. Gastric carcinoma cells were tested in vitro for cytotoxicity, Ao/EtBr staining for apoptotic cells, apoptotic DNA fragmentation in response to Coleus forskohlii extract. The protein expression of Bax, Bcl2 and Caspase-3 were analyzed through Western blotting. Treatment with Coleus forskohlii extract inhibited AGS cell proliferation dose and time dependently. Cells exposed to Coleus forskohlii extract showed typical hallmarks of apoptotic cell death. In conclusion, Coleus forskohlii ethanol extract has cytotoxic and apoptotic effects on Gastric carcinoma cells. Its mechanism of action might be associated with the apoptosis induction through up-regulation of the protein expressions of caspase-3 downregulation of the protein expression of Bax and Bcl2.