Bcl6 for identification of germinal centres in salivary gland biopsies in primary Sjögren's syndrome

Nakshbandi, Uzma; Haacke, Erlin A.; Bootsma, Hendrika; Vissink, Arjan; Spijkervet, Fred K. L.; Vegt, Bert van der; Kroese, Frans G. M.

doi:10.1111/odi.13276

Cited by 10 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lymphoepithelial lesions were determined by the presence of lymphocytes within the ductal epithelium with concurrent epithelial hyperplasia (Figure 1A,1B). A cluster of ≥5 Bcl6 + cells, lying next to each other within a focus was classified as germinal center (Figure 1C) [31,32]. Presence of a plasma cell shift was assessed using IgA/IgG double staining and IgM staining (Figure 1D).…”

Section: Histological Analysismentioning

confidence: 99%

Histopathology, salivary flow and ultrasonography of the parotid gland: three complementary measurements in primary Sjögren’s syndrome

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objective The involvement of salivary glands in primary Sjögren’s syndrome (pSS) can be assessed in different ways: histopathology, salivary flow and ultrasonography. To understand the relative value of these different approaches, it is crucial to understand the relationship between them. As we routinely perform these three modalities in the parotid gland for disease evaluation, our aim was to investigate the construct validity between these modalities in one and the same gland. Methods Consecutive sicca patients underwent a multidisciplinary diagnostic work-up including parotid gland biopsy, collection of parotid gland-specific saliva and parotid gland ultrasonography. Patients who were classified as pSS according to the ACR-EULAR criteria were included. Construct validity was assessed using Spearman’s correlation coefficients. Results The 41 included pSS patients completed a full work-up within mean time interval of 2.6 months. Correlations between histopathological features and stimulated parotid salivary flow were fair (ρ=-0.123 for focus score, and ρ=-0.259 for percentage of CD45+ infiltrate). Likewise, poor correlations were observed between stimulated parotid salivary flow and parotid ultrasonography (ρ=-0.196). Moderate to good associations were found between the histopathological items focus score and percentage of CD45+ infiltrate, with parotid ultrasound scores (total ultrasound score: ρ = 0.510 and ρ = 0.560; highest for homogeneity: ρ = 0.574 and ρ = 0.633). Conclusion Although pSS associated ultrasonographic findings did correlate with histopathological features, the three modalities that evaluate salivary gland involvement assess different (or at best partly related) constructs. Therefore, histopathology, salivary flow and ultrasonography are complementary measurements and cannot directly replace each other in the work-up of pSS.

show abstract

Section: Histological Analysismentioning

confidence: 99%

Histopathology, salivary flow and ultrasonography of the parotid gland: three complementary measurements in primary Sjögren’s syndrome

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Both ( B ) CD3+ T-lymphocytes and ( C ) CD20+ B-lymphocytes are present in the periductal infiltrate and within the ductal epithelium. ( D ) Presence of a germinal center, which was revealed by the presence of a cluster of ≥5 adjacent Bcl6-positive cells within a focus [ 28 ]. ( E ) Immunoglobulin A (IgA) (red) and immunoglobulin G (IgG) (brown) staining shows a plasma cell shift towards IgG plasma cells.…”

Section: Figurementioning

confidence: 99%

Imaging in Primary Sjögren’s Syndrome

Ginkel

Glaudemans

Vegt

et al. 2020

JCM

Self Cite

View full text Add to dashboard Cite

Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease characterized by dysfunction and lymphocytic infiltration of the salivary and lacrimal glands. Besides the characteristic sicca complaints, pSS patients can present a spectrum of signs and symptoms, which challenges the diagnostic process. Various imaging techniques can be used to assist in the diagnostic work-up and follow-up of pSS patients. Developments in imaging techniques provide new opportunities and perspectives. In this descriptive review, we discuss imaging techniques that are used in pSS with a focus on the salivary glands. The emphasis is on the contribution of these techniques to the diagnosis of pSS, their potential in assessing disease activity and disease progression in pSS, and their contribution to diagnosing and staging of pSS-associated lymphomas. Imaging findings of the salivary glands will be linked to histopathological changes in the salivary glands of pSS patients.

show abstract

“…Particular attention has been paid to the role of (glandular) B cells in pSS SG pathogenesis. B cells can form germinal centers, located within the foci, and roughly one-quarter of salivary glands of pSS patients have germinal centers (8). Whether autoreactive memory B cells are generated at these sites has been suggested, but formal proof is lacking.…”

Section: The Salivary Gland In Pssmentioning

confidence: 99%

The lacrimal gland in Sjögren's syndrome: can we unravel its mystery using ultrasound?

Yang

Delli

Coumou

et al. 2022

Clinical and Experimental Rheumatology

View full text Add to dashboard Cite

According to a recent survey of patients with the autoimmune disease primary Sjögren's syndrome (pSS), dry eye symptoms are present in 95-98% of pSS patients. As one of the most disabling symptoms mentioned by pSS patients, dry eyes have demonstrable effects on quality of their life, leading to eye dryness, itching, and pain, with some patients describing as a recurrent sensation of sand or gravel in the eyes. The symptoms are matched only in prevalence by dry mouth and chronic fatigue. In contrast to the prevalence of dry eye symptoms in pSS and their burden on pSS patients, our comprehension of dry eye disease development is minimal; specifically how function of the tear-fluid producing gland the lacrimal gland (LG), manifests. The comparison becomes stronger again when we consider what we know about dysfunction of the salivary gland (SG) in pSS, for example the appreciation of the transcriptome of 'innately activated' B cells invading the SG, their complicity in formation of lymphoepithelial lesions, and the ability of the SG epithelium to actively contribute to the inflammatory milieu. The exploration of ultrasound imaging as an additional modality to garner information about SG dysfunction in pSS has opened many doors for non-invasive, repeatable imaging in pSS.Here we summarize SG histology and ultrasound phenotype briefly and then juxtapose this with available studies examining LG pathology and ultrasound, and our understanding of LG dysfunction in pSS.

show abstract

Bcl6 for identification of germinal centres in salivary gland biopsies in primary Sjögren's syndrome

Cited by 10 publications

References 20 publications

Histopathology, salivary flow and ultrasonography of the parotid gland: three complementary measurements in primary Sjögren’s syndrome

Histopathology, salivary flow and ultrasonography of the parotid gland: three complementary measurements in primary Sjögren’s syndrome

Imaging in Primary Sjögren’s Syndrome

The lacrimal gland in Sjögren's syndrome: can we unravel its mystery using ultrasound?

Contact Info

Product

Resources

About