Bead affinity chromatography in a temperature-controllable microsystem for biomarker detection

Koh, Yul; Lee, Bo-Rahm; Yoon, Hyun Joong; Jang, Yun-Ho; Lee, Jun Young; Kim, Yong-Kweon; Kim, Byung‐Gee

doi:10.1007/s00216-012-6380-1

Cited by 20 publications

(13 citation statements)

References 26 publications

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“…[1][2][3][4][5][6] Many methods are available for protein detection, such as enzyme-linked immunosorbent assay (ELISA), 7-9 electrophoresis, 10-12 chromatography, [13][14][15] and mass spectrometry (MS). [1][2][3][4][5][6] Many methods are available for protein detection, such as enzyme-linked immunosorbent assay (ELISA), 7-9 electrophoresis, 10-12 chromatography, [13][14][15] and mass spectrometry (MS).…”

Section: Introductionmentioning

confidence: 99%

Using metal nanoparticles as a visual sensor for the discrimination of proteins

Liu

Hua

et al. 2014

J. Mater. Chem. B

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Protein discrimination is increasingly studied because of its roles in biological chemistry. In this study, we introduced a visual sensor using the metal nanoparticles (NPs) of Au, Ag, Cu, Ni and Co to determine the fluorescence (FL) intensity and colour changing patterns to identify ten types of proteins in a polyacrylamide gel. The synthesised NPs have little to no fluorescence, and the addition of proteins immobilised by the gel could differentially enhance the FL intensity and change the FL colour of the NPs, thereby producing FL recognition patterns. Based on the different FL enhancement indices of metal NPs with proteins, linear discriminant analysis (LDA) is applied to differentiate the proteins. By altering the synthesis method of metal NPs, a sensor with different FL patterns for protein discrimination is created.In addition, we have applied this method to detect the protein mixtures after one-dimensional (1-D) polyacrylamide gel electrophoresis (PAGE), and to evaluate the serum samples from patients with liver diseases and thalassemia and from healthy people. This protein discrimination method is rapid, costeffective and visual, and it has a "turn on" effect for metal NPs, which may be extended to other proteins or more complex bioanalytes. † Electronic supplementary information (ESI) available: Preparation for one-dimensional polyacrylamide gel electrophoresis (1-D PAGE) and metal NPs-based FL imaging, basic properties of proteins, all the uorescence data, preparation of ve colloidal metal NPs, the emission uorescence spectra and the UV-vis absorption spectrum, the differentiation of NPs, quantitative analysis of the proteins, FL change proles of NPs in the presence of gel components, and the discrimination of serum samples by the metal NPs based on gel. See

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Section: Introductionmentioning

confidence: 99%

Using metal nanoparticles as a visual sensor for the discrimination of proteins

Liu

Hua

et al. 2014

J. Mater. Chem. B

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“…Samples are introduced into the system by continuous flow sampling [ 49 – 57 , 59 – 66 , 68 – 70 , 72 – 76 , 80 – 83 , 89 , 91 , 93 – 96 , 98 – 102 , 104 , 106 ] or droplets or injection plugs [ 67 , 71 , 77 – 79 , 84 – 88 , 90 , 92 , 97 , 105 ] and subject to on-line manipulations such as separation [ 52 – 55 , 57 , 58 , 60 – 63 , 65 – 67 , 72 , 74 , 75 , 80 – 83 , 85 , 87 – 92 , 94 , 99 , 100 , 102 , 104 , 105 ] and digestion [ 49 , 50 , 68 , 72 , 73 , 83 , 94 ]. Samples can be transferred off-chip by electrospray/nano-electrospray [ 49 , 51 , 54 – 57 , 59 – 66 , 68 , 70 , 72 , 74 , 77 , 82 – 92 , 95 …”

Section: Summary Of Samples and Targeted Analytesmentioning

confidence: 99%

Interfacing microfluidics with information-rich detection systems for cells, bioparticles, and molecules

Smithers

Hayes

2022

Anal Bioanal Chem

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The development of elegant and numerous microfluidic manipulations has enabled significant advances in the processing of small volume samples and the detection of minute amounts of biomaterials. Effective isolation of single cells in a defined volume as well as manipulations of complex bioparticle or biomolecule mixtures allows for the utilization of information-rich detection methods including mass spectrometry, electron microscopy imaging, and amplification/sequencing. The art and science of translating biosamples from microfluidic platforms to highly advanced, information-rich detection system is the focus of this review, where we term the translation between the microfluidics elements to the external world “off-chipping.” When presented with the challenge of presenting sub-nanoliter volumes of manipulated sample to a detection scheme, several delivery techniques have been developed for effective analysis. These techniques include spraying (electrospray, nano-electrospray, pneumatic), meniscus-defined volumes (droplets, plugs), constrained volumes (narrow channels, containers), and phase changes (deposition, freezing). Each technique has been proven effective in delivering highly defined samples from microfluidic systems to the detection elements. This review organizes and presents selective publications that illustrate the advancements of these delivery techniques with respect to the type of sample analyzed, while introducing each strategy and providing historical perspective. The publications highlighted in this review were chosen due to their significance and relevance in the development of their respective off-chip technique. Graphical abstract This review highlights advancements of delivery methods off a microfluidic chip for additional information rich detection schemes Supplementary Information The online version contains supplementary material available at 10.1007/s00216-022-04043-1.

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“…Recently, a temperature‐controllable bead affinity chromatography in a microfluidic system for sample purification and preparation was reported (Koh et al, ). Carcinoembryonic antigen was successfully concentrated and purified from human serum using the developed system and detected by MALDI‐MS.…”

Section: Microfluidic Chip Coupling To Maldi‐msmentioning

confidence: 99%

Advances in coupling microfluidic chips to mass spectrometry

et al. 2014

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Microfluidic technology has shown advantages of low sample consumption, reduced analysis time, high throughput, and potential for integration and automation. Coupling microfluidic chips to mass spectrometry (Chip-MS) can greatly improve the overall analytical performance of MS-based approaches and expand their potential applications. In this article, we review the advances of Chip-MS in the past decade, covering innovations in microchip fabrication, microchips coupled to electrospray ionization (ESI)-MS and matrix-assisted laser desorption/ionization (MALDI)-MS. Development of integrated microfluidic systems for automated MS analysis will be further documented, as well as recent applications of Chip-MS in proteomics, metabolomics, cell analysis, and clinical diagnosis.

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Bead affinity chromatography in a temperature-controllable microsystem for biomarker detection

Cited by 20 publications

References 26 publications

Using metal nanoparticles as a visual sensor for the discrimination of proteins

Using metal nanoparticles as a visual sensor for the discrimination of proteins

Interfacing microfluidics with information-rich detection systems for cells, bioparticles, and molecules

Advances in coupling microfluidic chips to mass spectrometry

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