Bead form of guaran gel for chromatographic column packing

Kumar, Sanjeev; Chandra, Satya V.; Kanoongo, Neeta; Mathur, V.; Mathur, Nupur

doi:10.1016/0923-1137(93)90061-j

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1998

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“…liquid chromatography (HPLC) as a column packing material [40,41] In the present paper we report the use of guaran as a buffer modifier in CE to improve the separation of basic proteins and drugs. When 0.1% (w/v) guaran was added to the running buffer, the peak efficiency of proteins and beta-blockers was dramatically improved.…”

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoretic separation of basic proteins and drugs using guaran as a buffer modifier

Liu

Lin

Hartwick³

1998

Chromatographia

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SummaryGuaran, a neutral polysaccharide, has been used as a buffer modifier to improve the separation of basic proteins and drugs. Migration reproducibility, peak shape and efficiency were improved when 0.1% guaran was added to the buffer. The concentration of guaran, ionic strength, and pH of buffer solution were optimized to obtain the optimum separation of proteins. Possible separation efficiencies of 700,000 plates per meter were obtained for test proteins. The relative standard deviation (%RSD) of the migration time of all test proteins was less than 0.5 %. Improved separation of [3-blockers was also observed when guaran was added to the buffer.

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Section: Introductionmentioning

confidence: 99%