Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

Amobonye, Ayodeji; Bhagwat, Prashant; Singh, Suren

doi:10.3390/jof7080668

Cited by 12 publications

(7 citation statements)

References 62 publications

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“…The molecular mass of partially purified xylanase was determined to be 35 kDa based on the collected fractions and the activity and position of the bands in the electropherogram. The molecular sizes of xylanases isolated from fungi ranged from negligible to very large; however, the results showed good agreement with the molecular sizes of xylanases isolated from A. oryzae and Beauveria bassiana (37 kDa) ( 37 , 38 ). The fractions in which xylanase activity was detected were pooled, freeze-dried and used for further enzyme characterisation.…”

Section: Resultssupporting

confidence: 53%

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Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Šekuljica,

Jakovetić Tanasković,

Mijalković

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. The development of a novel process for the production of xylooligosaccharides based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification, together with the physical pretreatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, xylooligosaccharides. Experimental approach. To produce xylanase, a solid-state fermentation was performed using wheat bran as substrate. In order to obtain the most active xylanase crude extract, the time frame of the cultivation process was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterization included determination of the optimal pH and temperature, determination of the molecular mass of the purified xylanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the partially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a 49 % yield and 13-fold xylanase purification was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/(min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyze the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chromatography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerization was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focused on creating new enzymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.

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Section: Resultssupporting

confidence: 53%

“…The results of this study are fully consistent with previously collected data on the pH ranges in which fungal-derived xylanases function best. There are a few xylanases that prefer an alkaline environment, but the vast majority of fungal-derived xylanases are acidophilic ( 37 ).…”

Section: Resultsmentioning

confidence: 99%

Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Šekuljica,

Jakovetić Tanasković,

Mijalković

et al. 2023

Food Technol. Biotechnol. (Online)

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“…The strain was selected based on its ability to utilise readily available agricultural residues for its growth as well as its remarkable enzyme production ability. It was initially cultured on potato dextrose agar (PDA) at 30 °C for five days, and the spore suspension (1 × 10 6 spores mL −1 ) was prepared following the protocol of Amobonye, Bhagwat, Singh and Pillai [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Magwaza,

Amobonye,

Bhagwat

et al. 2024

Heliyon

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“…A mathematical modeling and statistical method called the Box–Behnken design (BBD) of RSM aids in optimizing conditions [ 43 ]. Previous studies used a RSM to optimize biological processes [ 44 , 45 , 46 , 47 , 48 ], BBD also has been applied in the optimization of the yield of EPF in several studies [ 40 , 49 , 50 ]. However, it has not been applied to entomopathogenic Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass

et al. 2023

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Fusarium equiseti (JMF-01), as an entomopathogenic fungus, can effectively control agricultural pests and has the potential to be a biocontrol agent. To promote mycelial growth and sporulation, we investigated the optimal submerged culture conditions for F. equiseti. In this study, we used the single-factor method and Box–Behnken design and determined the virulence of the submerged culture against Myzus persicae after optimization. As a result, the highly significant factors affecting the spore concentration of strain JMF-01 were the primary inoculum density and the initial pH, and the highly significant factor affecting the mycelial biomass was the medium-to-flask ratio. The highest mycelial biomass value was 0.35 g when the incubation time was 5.68 days, the initial pH was 5.11, the medium-to-flask ratio was 0.43, and 1 mL of the primary inoculum with spore density of 0.97 × 107 conidia/mL was added. When the incubation time was 6.32 days, the initial pH was 4.46, the medium-to-flask ratio was 0.35, the primary inoculum density was 1.32 × 107 conidia/mL of 1 mL, and the highest spore concentration of 6.49 × 108 blastospores/mL was obtained. Compared with the unoptimized medium conditions, the optimized submerged culture had the highest mycelial biomass and spore concentration, which were 3.46 and 2.06 times higher, respectively. The optimized submerged culture was highly pathogenic toward M. persicae, reaching a 95% mortality rate. Our results provide optimal submerged culture conditions for F. equiseti and lay the basis for later research to expand production for pest control.

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Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

Cited by 12 publications

References 62 publications

Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass

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