Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae)

Alves, Sérgio Batista; Rossi, Luciana Savoi; Lopes, Rogério Biaggioni; Tamai, M. A.; Pereira, Roberto M.

doi:10.1016/s0022-2011(02)00147-7

Cited by 104 publications

(68 citation statements)

References 13 publications

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“…The extracellular proteases are considered an important virulence factor in insect disease processes (St. Leger et al, 1986a, 1986b1988;Draganova, 1988;Bidochka and Khachatourians, 1990, 1994a, 1994bGupta et al, 1992;St. Leger, 1995).…”

Section: Resultsmentioning

confidence: 99%

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Draganova

Simova

2010

PESTIC PHYTOMED

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SUMMARYBioassays with five isolates of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuillemin were conducted under laboratory conditions with a goal to estimate their virulence to the two-spotted spider mite Tetranychus urticae Koch. (Acari: Tetranychidae). Common bean plants with mites were treated by spraying conidial suspensions of isolates at concentrations of 10 6 , 10 7 and 10 8 conidia/ml. Lethal effects of the fungal isolates were evaluated as percentages of cumulative daily mortality due to mycoses, corrected for mortality in the control variant. Virulence of the isolates was estimated based on values of the median lethal time (LT 50 ) calculated by probit analysis for the variants treated with conidial suspensions at the concentration of 10 6 conidia/ml.The two-spotted spider mite was found susceptible to the examined isolates of the entomopathogenic fungus B. bassiana.Mycosis caused to T. urticae by the B. bassiana isolates 444 Bb and 445 Bb had fast lethal effect after treatment with conidial suspensions even at the concentration of 10 6 conidia/ ml. The mean mortality values of host individuals were 83.78 ± 3.62% and 68.49 ± 4.28% on the first day, respectively, and up to 100% in both variants on the fourth day.The isolates 426 Bb, 444 Bb and 445 Bb of B. bassiana were highly virulent to two-spotted spider mites with values of the median lethal time varied within overlapped narrow confidence intervals from 0.122 to 1.084 days (average value 0.162 days), from 0.117 to 1.398 days (average value 0.146 days) and from 0.106 to 1.162 days (average value 0.131 days), respectively. Significant differences regarding virulence of the three isolates at p-level < 0.05 could not been proved. The other two examined isolates were distinctly less virulent to T. urticae than these three B. bassiana isolates.

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Section: Resultsmentioning

confidence: 99%

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Draganova

Simova

2010

PESTIC PHYTOMED

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“…Radial extension rate was considered as an estimation of the fungal ability to colonize the substrate on which it was growing. The values for radial extension rate markedly enhanced (between 10 and 15%) after the addition of liquid media reported by Alves et al (2002) or a standard Dextrose Sabouraud broth. This behavior was similar to that reported by Kamp and Bidochka (2002) who found better growth for B. bassiana in more complex media.…”

Section: Effect Of Water Activitymentioning

confidence: 91%

“…For both conidial density and radial extension rate assays, conidia were point inoculated in triplicate in the center of Petri dishes containing wheat bran (10%) as the only nutrient source plus agar (15 g/L). This medium was also supplemented either with components of a synthetic medium (Alves et al 2002) or standard 4% (w/v) Dextrose Sabouraud broth diluted (1:2) previously. Ethylene glycol (0 to 20%) was used as a water activity (a w ) depressor in these media (Montiel-González et al 2004), which allowed the modification of a w values within a range of 1.0 to 0.93.…”

Section: Microorganism Propagationmentioning

confidence: 99%

Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

Ñuñez-Gaona

Saucedo‐Castañeda

Alatorre-Rosas

et al. 2010

Braz. arch. biol. technol.

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“…are popular entomopathogenic fungi, which have been broadly called dongchunghacho (winter worm summer grass) in Korea (8)(9)(10)(11). Paecilomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Lee

Park

Son

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. the aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentrationdependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (mmp-9), a 92-kda gelatinase. taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of mmp-9 expression in B16F10 cells. Introductioncancer is a progressive disease that affects many types of tissues, as well as organs, in the human body (1). cancer progression and metastasis is a complicated process. cells move through the blood and invade neighboring tissue by degrading the extracellular matrix (Ecm) (2). in these multiprocesses, angiogenesis plays a pivotal role in the formation of new blood vessels in the newly colonized areas, and thus in the formation of additional metastatic lesions (3,4). the Ecm includes a wide variety of proteins, which can be degraded by many matrix metalloproteinases (mmps). mmps are a family of highly conserved zinc-dependent peptidases capable of degrading the Ecm (3).anti-tumor agents have been shown to inhibit mmp-9 activity in the Ecm via the binding of the nF-κB transcription factor with the mmp-9 promoter (5). this suggests that anti-tumor agents play a role in anti-invasion and/or anti-angiogenesis, as well as in caspase-3 inhibition (6). cell motility, and in particular cell migration, affect tumor progression and metastasis (7). the signaling pathways responsible for regulating cell motility involve various nuclear transcription factors, as well as morphological changes in the cytoskeleton that modify interactions among membrane-and Ecm-bound proteins (e.g., cdc, rho and rac family proteins), which mediate cellular and membrane morphology by controlling the polymerization-depolymerization process of the actin cytoskeleton (8).Cordyceps and Paecilomyces sp. are popular entomopathogenic fungi, which have been broadly called dongchunghacho (winter worm summer grass) in Korea (8-11). Paecilomyces sp. has been investigated for industrial purposes in order to be cultivated on a large scale. however, the precise biological mechanisms of functionality and bioavailability require investigation.in this study, we show that Paecilomyces farinosus J3, which was collected and isolated from mansusan, Korea, decreases cell migration, invasion and tube formation, suggesting that this activity may have originated from the pathway of mmp-9 inhibition. Our findings, in combination with previous studies, strongly suggest that Paecilomyces farinosus J3 should be considered a good candidate for ...

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Beauveria bassiana yeast phase on agar medium and its pathogenicity against Diatraea saccharalis (Lepidoptera: Crambidae) and Tetranychus urticae (Acari: Tetranychidae)

Cited by 104 publications

References 13 publications

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

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