The aim of this study is to determine adolescents' trait anxiety levels during physical education lessons and investigate them by a range of variables. Screening model was used in the study. The study group was a total of 1083 high school students (M age =16.08, SD=1.11), including 491 females (45.3%) and 592 males (%54.7), from high schools in the provinces of Ankara, Aksaray and Istanbul during the academic year 2013-2014. "Physical Education Trait Anxiety Scale" was used to collect data. In the analyses, in addition to descriptive statistics, independent sample t-test of the difference between the arithmetic means and one-way analysis of variance were used. In the study, students were found to have a moderate level of physical education trait anxiety. Significant differences were observed by gender and grade in physical education trait anxiety levels, while no significant difference was found by being licensed athlete.