Bedeutung und Herkunft der Enzyme im Glask�rper des Rindes

Hoffmann, Karl; Wurster, Ulrich

doi:10.1007/bf00414338

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…This does not imply, however, that the vitreous cortex cells do not elevate the enzyme activity of the homogenate at all; their contribution is merely too small to manifest itself statistically. In fact, it had been shown previously (Hoffmann & Wurster 1974b), that separate homogenization of the high-speed hyalocyte sediment of calves and re-addition to the supernatant results in an activity increase of about 10% which is well within the confidence limits set by the natural biological variation. Nevertheless, contribution of vitreous cortex cells may become significant for smaller eyes.…”

Section: Discussionmentioning

confidence: 71%

“…What remains is the influence of collecting, processing and storing of the samples as well as the age and possibly sex of the probands. Earlier work had revealed no influence of age on LDH and MDH in the bovine vitreous (Hoffmann & Wurster 1974b) or aqueous (Hockwin & Kietzmann 1978) while Osterlin (1975) reported a decrease in the specific activity of cathepsin D. Enzyme activities are based on volume rather than protein in the present study to exclude effects due to alterations confined to (bulk) protein only. In their comparative study Balazs et al (1959) indicated that some of their values for soluble protein, especially for smaller animals, might be erroneously high.…”

Section: Discussionmentioning

confidence: 81%

“…1.1.1.37, MDH) and phosphoglucoseisomerase (E.C. 5.3.1.9, PGI) have been described before (Hoffmann & Wurster 1974b). …”

Section: Methodsmentioning

confidence: 98%

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Enzyme Activities and Protein Concentration in the Intraocular Fluids of Ten Mammals

Wurster

Riese

Hoffmann

1982

Acta Ophthalmologica

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An attempt was made to establish normal values for the total protein concentrations and the enzyme activities of LDH, MDH and PGI in the intraocular fluids of rats, guinea pigs, rabbits, cats, dogs, sheep, cattle, pigs, horses and humans. Remarkably little species differences were noted in 9 of the 10 mammals with vitreal enzyme activities falling into a narrow range between 8.4 U/l (PGI, horse) and 92.4 U/l (MDH, guinea pig). All species obeyed the sequence aqueous less than vitreous less than serum with exception of the rat, where vitreous activities surpassed serum at least two-fold. The very low enzyme activities in the aqueous and vitreous humors demand special precautions against contaminations which can either derive from post-mortem storage or inadequate sampling procedures. Conversely elevated enzyme activities could be used as probes for pathological processes or as a check for a clean preparation.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 81%

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Enzyme Activities and Protein Concentration in the Intraocular Fluids of Ten Mammals

Wurster

Riese

Hoffmann

1982

Acta Ophthalmologica

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“…Vitreous gel contains a variety of organic and anorganic components necessary for bacterial growth (Bourwieg, Hoffmann and Riese, 1974 ;Hoffmann and Wurster, 1974 ;Coutselinis, Boukis and Kalofontis, 1977 ;Balazs and Dendlinger, 1984). The protein rate (0n1-0n6 g l −" ) in vitreous is very low, due to the impermeability of the blood retinal barrier to higher molecular substances (Scott, 1992).…”

Section: Introductionmentioning

confidence: 99%