Bedside Testing for Chronic Pelvic Pain: Discriminating Visceral from Somatic Pain

Jarrell, John; Giamberardino, Maria Adele; Robert, Magali; Nasr-Esfahani, Maryam

doi:10.1155/2011/692102

Cited by 29 publications

(36 citation statements)

References 25 publications

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“…Jarrell et al have shown that abdominal cutaneous allodynia, reduced pain thresholds, and abdominal wall trigger points are associated with pelvic pain of visceral origin. 20 We do not have data on cutaneous allodynia or pain thresholds, but our observed association between pelvic floor tenderness and abdominal wall pain (trigger points) confirms a role of visceral pain in our population. Our observation of an association between pelvic floor tenderness and bladder base tenderness also confirms the bladder as another source of visceral pain in this population.…”

Section: Discussionsupporting

confidence: 45%

Pelvic Floor Tenderness in the Etiology of Superficial Dyspareunia

Yong¹,

Mui²,

Allaire³

et al. 2014

Journal of Obstetrics and Gynaecology Canada

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Section: Discussionsupporting

confidence: 45%

Pelvic Floor Tenderness in the Etiology of Superficial Dyspareunia

Yong¹,

Mui²,

Allaire³

et al. 2014

Journal of Obstetrics and Gynaecology Canada

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“…To study this, we infected donor NOD mice with CP-1, isolated serum and T cells (Pan T) and transferred them into naïve NOD mice (five per group) that were unexposed to CP-1. Recipient mice were tested at baseline and over time for the development of suprapubic tactile allodynia, a consequence of referred hyperalgesia and a characteristic of visceral pain [10]–[12]. While tactile allodynia was not changed in the group administered immune serum or naïve serum (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Behavior testing was based on the concept of cutaneous hyperalgesia resulting from referred visceral pain [10]–[12]. An irritable focus in visceral tissues reduces cutaneous pain thresholds allowing for an exaggerated response to normally non-painful stimuli (allodynia).…”

Section: Methodsmentioning

confidence: 99%

Th1-Th17 Cells Contribute to the Development of Uropathogenic Escherichia coli-Induced Chronic Pelvic Pain

et al. 2013

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The etiology of chronic prostatitis/chronic pelvic pain syndrome in men is unknown but may involve microbes and autoimmune mechanisms. We developed an infection model of chronic pelvic pain in NOD/ShiLtJ (NOD) mice with a clinical Escherichia coli isolate (CP-1) from a patient with chronic pelvic pain. We investigated pain mechanisms in NOD mice and compared it to C57BL/6 (B6) mice, a strain resistant to CP-1-induced pain. Adoptive transfer of CD4+ T cells, but not serum, from CP-1-infected NOD mice was sufficient to induce chronic pelvic pain. CD4+ T cells in CP-1-infected NOD mice expressed IFN-γ and IL-17A but not IL-4, consistent with a Th1/Th17 immune signature. Adoptive transfer of ex-vivo expanded IFN-γ or IL-17A-expressing cells was sufficient to induce pelvic pain in naïve NOD recipients. Pelvic pain was not abolished in NOD-IFN-γ-KO mice but was associated with an enhanced IL-17A immune response to CP1 infection. These findings demonstrate a novel role for Th1 and Th17-mediated adaptive immune mechanisms in chronic pelvic pain.

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“…Behavior testing was based on the concept of cutaneous hyperalgesia resulting from referred visceral pain [8][9][10] . An irritable focus in visceral tissues reduces cutaneous pain thresholds allowing for an exaggerated response to normally non-painful stimuli (allodynia).…”

Section: Introductionmentioning

confidence: 99%

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

Quick

Done

Thumbikat

2013

JoVE

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Uropathogenic Escherichia coli (UPEC) are pathogens that play an important role in urinary tract infections and bacterial prostatitis 1 . We have recently shown that UPEC have an important role in the initiation of chronic pelvic pain 2 , a feature of Chronic prostatitis/Chronic pelvic pain syndrome (CP/CPPS) 3,4 . Infection of the prostate by clinically relevant UPEC can initiate and establish chronic pain through mechanisms that may involve tissue damage and the initiation of mechanisms of autoimmunity 5 .A challenge to understanding the pathogenesis of UPEC in the prostate is the relative inaccessibility of the prostate gland to manipulation. We utilized a previously described intraurethral infection method 6 to deliver a clinical strain of UPEC into male mice thereby establishing an ascending infection of the prostate. Here, we describe our protocols for standardizing the bacterial inoculum 7 as well as the procedure for catheterizing anesthetized male mice for instillation of bacteria.CP/CPPS is primarily characterized by the presence of tactile allodynia 4 . Behavior testing was based on the concept of cutaneous hyperalgesia resulting from referred visceral pain [8][9][10] . An irritable focus in visceral tissues reduces cutaneous pain thresholds allowing for an exaggerated response to normally non-painful stimuli (allodynia). Application of normal force to the skin result in abnormal responses that tend to increase with the intensity of the underlying visceral pain. We describe methodology in NOD/ShiLtJ mice that utilize von Frey fibers to quantify tactile allodynia over time in response to a single infection with UPEC bacteria. Video LinkThe video component of this article can be found at http://www.jove.com/video/50158/ Protocol Bacteria Preparation for Mouse InoculationThe following must be done under aseptic conditions. 1. Take one 17 x 100 mm polypropylene tubes with caps and add 3 ml of fresh Luria broth (LB) media. Using autoclaved tips, take some frozen bacteria glycerol stock of strain CP1 2 and transfer a small quantity of the culture into the tube containing the LB media. Replace the tube cap so that oxygen is still able to enter the tube -the culture needs to grow under aerobic conditions. Place tube at 37 °C in a shaking incubator at 220 rpm overnight. 2. The next day, transfer 5 μl of overnight culture to a fresh tube with 3 ml LB media and grow under static conditions in an incubator overnight at 37 °C. 3. On day 3 transfer 40 μl of culture into a a 50 ml tube containing 40 ml LB media each. Place in a 37 °C static incubator overnight. 4. Turn on and set centrifuge to 4 °C. Once centrifuge has reached final temperature, transfer each 40 ml culture into a 40 ml Nalgene centrifuge tube. 5. Weigh tubes to balance centrifuge -adjust volume with LB media as needed. Spin tubes at 6,000 rpm for 20 min. 6. Carefully remove supernatant and gently suspend bacterial pellet in 40 ml sterile PBS. 7. Repeat step 1.5 with PBS. 8. Aspirate off supernatant and suspend bacteria in 500 μl sterile PBS....

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Bedside Testing for Chronic Pelvic Pain: Discriminating Visceral from Somatic Pain

Cited by 29 publications

References 25 publications

Pelvic Floor Tenderness in the Etiology of Superficial Dyspareunia

Pelvic Floor Tenderness in the Etiology of Superficial Dyspareunia

Th1-Th17 Cells Contribute to the Development of Uropathogenic Escherichia coli-Induced Chronic Pelvic Pain

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis

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