Beef-Induced Anaphylaxis Confirmed by the Basophil Activation Test

Kim, Joo Hee; An, Soyeon; Kim, Jeong Eun; Choi, Gil Soon; Ye, Young-Min; Park, Hae‐Sim

doi:10.4168/aair.2010.2.3.206

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…For the functional in vitro test for allergic reactions to cefaclor, a basophil activation test (BAT) was performed using flow cytometry (FACScantoII; BD Immunocytometry Systems, San Jose, CA, USA), using CD63 antibody according to previously described methods. 13 Patients' basophils were incubated with cefaclor at dilutions of 1.6×10 -3 , 1.6×10 -2 , and 1.6×10 -1 mg/mL for 30 minutes. BATs were conducted for two healthy controls under the same condition to exclude a nonspecific activation of basophil.…”

Section: Methodsmentioning

confidence: 99%

Immunologic Evaluation of Immediate Hypersensitivity to Cefaclor

et al. 2014

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PurposeCefaclor is widely prescribed for various infectious diseases. As its consumption increases, the number of hypersensitivity reactions to cefaclor has increased. This study aimed to evaluate the immunologic findings of immediate hypersensitivity to cefaclor.Materials and MethodsWe enrolled 47 patients with immediate hypersensitivity to cefaclor from Ajou University Hospital and Asan Medical Center. Serum specific IgE, IgG1, and IgG4 antibodies to cefaclor-human serum albumin (HSA) conjugate were measured by enzyme-linked immunosorbent assay (ELISA).ResultsThe most common phenotype was anaphylaxis (Group I, 78.7%), followed by urticaria (Group II, 21.3%). The detection of specific IgE, IgG1, and IgG4 to cefaclor-HSA conjugate by ELISA tended to be higher in Group I (40.5%, 41.7%, 21.6%) than in Group II (20.0%, 20.0%, 0%) with no statistical significance. Significant associations were found between specific IgE and IgG1 or IgG4 (p<0.001, p=0.019). ELISA inhibition tests showed significant inhibitions by both free cefaclor and cefaclor-HSA conjugate. For basophil activation tests in patients having no specific IgE antibody, the CD63 expression level on basophils increased with incubations of free cefaclor.ConclusionThe most common manifestation of immediate hypersensitivity to cefaclor was anaphylaxis, most of which was mediated by IgE; however, a non-IgE mediated direct basophil activation mechanism was suggested in a subset of anaphylaxis patients.

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Section: Methodsmentioning

confidence: 99%

Immunologic Evaluation of Immediate Hypersensitivity to Cefaclor

et al. 2014

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“…The rationale of this test is the change in the phenotype of activated basophils after in vitro incubation of the patient’s whole blood with the allergen. The BAT is a useful complementary tool to the in vitro diagnosis of FA caused by milk, egg, peanut and wheat [ 69 , 70 ] when IgE may be involved. Interestingly, a recent study of 20 peanut-allergic children showed that when basophils were stimulated with decreasing doses of allergens until threshold sensitivity was reached, 19 were negative to peanut but 17 were positive to rAra h 8, suggesting that the children sensitized to Ara h 8 but not peanut storage proteins may be at risk of systemic allergic reactions, especially when eating large amounts of peanuts [ 70 ].…”

Section: Diagnostic Methods For Food Allergymentioning

confidence: 99%

Guidelines for the use and interpretation of diagnostic methods in adult food allergy

et al. 2015

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Food allergy has an increasing prevalence in the general population and in Italy concerns 8 % of people with allergies. The spectrum of its clinical manifestations ranges from mild symptoms up to potentially fatal anaphylactic shock. A number of patients can be diagnosed easily by the use of first- and second-level procedures (history, skin tests and allergen specific IgE). Patients with complex presentation, such as multiple sensitizations and pollen-food syndromes, frequently require a third-level approach including molecular diagnostics, which enables the design of a component-resolved sensitization profile for each patient. The use of such techniques involves specialists’ and experts’ skills on the issue to appropriately meet the diagnostic and therapeutic needs of patients. Particularly, educational programs for allergists on the use and interpretation of molecular diagnostics are needed.

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“…The sensitivity of BAT to different drugs ranges between 36% and 97.7%, with a specificity of approximately 93% [150] . There was a case that the SPT and serum sIgE to beef, pork and milk allergens showed negative results, but the BAT showed significant positive results [152] . The positive SACTs will confirm the diagnosis of allergy, and the substances that the patients are allergic to.…”

Section: Allergen-specific Immunoglobulin Testsmentioning

confidence: 99%

Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis

Zhang

Zeng

et al. 2013

Acta Pharmacol Sin

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The current definition of allergy is a group of IgE-mediated diseases. However, a large portion of patients with clinical manifestations of allergies do not exhibit elevated serum levels of IgE (sIgEs). In this article, three key factors, ie soluble allergens, sIgEs and mast cells or basophils, representing the causative factors, messengers and primary effector cells in allergic inflammation, respectively, were discussed. Based on current knowledge on allergic diseases, we propose that allergic diseases are a group of diseases mediated through activated mast cells and/or basophils in sensitive individuals, and allergic diseases include four subgroups: (1) IgE dependent; (2) other immunoglobulin dependent; (3) non-immunoglobulin mediated; (4) mixture of the first three subgroups. According to our proposed definition, pseudo-allergic-reactions, in which mast cell or basophil activation is not mediated via IgE, or to a lesser extent via IgG or IgM, should be non-IgE-mediated allergic diseases. Specific allergen challenge tests (SACTs) are gold standard tests for diagnosing allergies in vivo, but risky. The identification of surface membrane activation markers of mast cells and basophils (CD203c, CCR3, CD63, etc) has led to development of the basophil activation test (BAT), an in vitro specific allergen challenge test (SACT). Based on currently available laboratory allergy tests, we here propose a laboratory examination procedure for allergy.

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Beef-Induced Anaphylaxis Confirmed by the Basophil Activation Test

Cited by 14 publications

References 10 publications

Immunologic Evaluation of Immediate Hypersensitivity to Cefaclor

Immunologic Evaluation of Immediate Hypersensitivity to Cefaclor

Guidelines for the use and interpretation of diagnostic methods in adult food allergy

Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis

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