Behavior of Adsorbed Albumin Film on CoCrMo Alloy under <I>In-situ</I> Observation

Abstract

The protein film adsorbed on joint prosthesis material affects tribological property. It is expected that optimum protein film structure could improve friction and wear of joint prosthesis materials. Therefore, it is important to understand and to clarify the effect of protein film on tribological property. In the rubbing combination of ultra-high molecular weight polyethylene (UHMWPE) and CoCrMo alloy, the adsorption behavior of bovine serum albumin (BSA) and its effect on frictional property during friction were investigated by using electrochemical method. Friction force and electric potential were measured under open circuit potential (OCP) condition, but friction force and electric current were measured under −0.2 V applied potential condition. Under OCP condition, adsorbed BSA was peeled off at first rubbing, after that BSA re-adsorbed on surface under shear force condition. BSA adsorbed film reconstructed by shear force was sustained during rubbing. It is considered that the conformation of adsorbed BSA under shear stress was changed from that of static adsorption, because readsorbed BSA molecule did not show exfoliation from metal surface, which means that adsorption strength was increased from static adsorption. Under applied potential condition where wear performance of BSA film is expected to be evaluated, BSA showed the desorption at first rubbing, and gradual decrease in desorption amount. Therefore, it is confirmed that BSA film on metal surface under rubbing condition is reconstructed during rubbing. The adsorbed film formed during rubbing is stable and has an optimally-adapted structure for shear force and can sustain its property during rubbing.

A systematic review on correlation between biochemical and mechanical processes of lubricant film formation in joint replacement of the last 10 years

“…4,10,12 Most of the studies found are concerned with adsorption and desorption of proteins in different kinematic conditions and film thickness in different pH, buffer, and concentration of proteins. 7,13,14,[16][17][18][19] Frictional coefficients, frictional force, shear force, wear measurement, boundary lubrication, surface geometry, rheology of various constituents are also considered in connection with proteins and other SF components to explore the knowledge in this area. 2,3,8,14,15,19,23 To take into account the mechanical and chemical environment of human synovial joint, the viscosity of various modal fluids and wettability of various surface materials were also considered 4,[15][16][17]23 with respect to their duration in time and under varying temperature and pressure.…”

“…Nakashima et al 14 studied the adsorption of BSA through measurement of friction force and electric potential under open circuit potential condition in the rubbing combination of ultrahigh molecular weight polyethylene and CoCrMo alloy. The results show the onset of BSA desorption at first rubbing with the gradual decline of desorption.…”

A Common Hydration Lubrication Based on Hydrophobically Modified Sulfobetaine Polyurethane and Synovial Components in the Application of Artificial Meniscus

“…Unexpectedly, the addition of HA did not affect the lubrication performance provided by PC layers according to Lin et al 33 SA is the most common protein in synovial fluid, 34 but its lubrication role remains controversial until now. 29,35 For example, Yoneyama et al 29 concluded that SA functioned as a lubrication role to reduce friction, while Nakashima et al 35 found that SA worsened the friction and wear of joint prosthesis materials.…”

Raman analysis of chemisorbed tribofilm for metal‐on‐polyethylene hip joint prostheses

“…Using real-time information about the adsorption and desorption of proteins, it was disclosed that during rubbing, the BSA film is reconstructed on the Co-Cr-Mo surface by shear force. By acquiring shear force, the adsorbed film becomes a strong, stable and optimally adapted structure [8]. Electrochemical corrosion resistance is a vital factor in the biocompatibility of metals.…”