Behavior of an Adsorbed Phospholipid Monolayer Submitted to Prolonged Periodical Surface Density Variations

Nguyen, Phuc Nghia; Waton, Gilles; Vandamme, Thierry F.; Krafft, Marie Pierre

doi:10.1002/ange.201301974

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…As summarized in Table 1, DPPC vesicles both adsorbed and spread onto the air-water surface result in an equilibrium surface tension of 41 mN/m at 37 C. This value is close to that obtained with the micropipette technique (28) but significantly lower than that obtained with various bubble methods (26,27). Nevertheless, it is found that only the DPPC monolayer spread from organic solvent is able to reach the intrinsic equilibrium surface tension of the phospholipid monolayer (i.e., $22 mN/m).…”

Section: Experimental Measurements Of the Surface Tension And Morphology Of Dppc Filmssupporting

confidence: 79%

“…The dynamic surface tension measurements showed significant discrepancies with different preparations of DPPC vesicles (25,29,30). Many measured equilibrium surface tensions were much higher than the theoretical equilibrium surface tension of the air-alkyl surface (21,(24)(25)(26)(28)(29)(30)(31). Infrared reflection-absorption spectroscopy results showed that there were more than one monolayer at the air-water surface after adsorption of the DPPC vesicles (25,29,30).…”

Section: Introductionmentioning

confidence: 92%

“…Despite numerous experimental studies of lipid adsorption (22)(23)(24)(25)(26), the detailed biophysical mechanism of lipid vesicle adsorption is still unclear. Various experimental methodologies, including Langmuir balance (12), pulsating bubble surfactometry (27), captive bubble surfactometry (22), micropipette technique (28), and infrared reflectionabsorption spectroscopy (25), have been used for measuring the dynamic surface tension and the structure of adsorbed phospholipid films.…”

Section: Introductionmentioning

confidence: 99%

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Adsorption of Phospholipids at the Air-Water Surface

Bai

Tang

et al. 2019

Biophysical Journal

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Phospholipids are ubiquitous components of biomembranes and common biomaterials used in many bioengineering applications. Understanding adsorption of phospholipids at the air-water surface plays an important role in the study of pulmonary surfactants and cell membranes. To date, however, the biophysical mechanisms of phospholipid adsorption are still unknown. It is challenging to reveal the molecular structure of adsorbed phospholipid films. Using combined experiments with constrained drop surfactometry and molecular dynamics simulations, here, we studied the biophysical mechanisms of dipalmitoylphosphatidylcholine (DPPC) adsorption at the air-water surface. It was found that the DPPC film adsorbed from vesicles showed distinct equilibrium surface tensions from the DPPC monolayer spread via organic solvents. Our simulations revealed that only the outer leaflet of the DPPC vesicle is capable of unzipping and spreading at the air-water surface, whereas the inner leaflet remains intact and forms an inverted micelle to the interfacial monolayer. This inverted micelle increases the local curvature of the monolayer, thus leading to a loosely packed monolayer at the air-water surface and hence a higher equilibrium surface tension. These findings provide novel insights, to our knowledge, into the mechanism of the phospholipid and pulmonary surfactant adsorption and may help understand the structure-function correlation in biomembranes.

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Section: Experimental Measurements Of the Surface Tension And Morphology Of Dppc Filmssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Adsorption of Phospholipids at the Air-Water Surface

Bai

Tang

et al. 2019

Biophysical Journal

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“…In the case of sub-millimeter-sized microbubbles shelled with phospholipids, molecular adsorption to the gas−liquid interface can be evaluated by bubble profile analysis tensiometry. 36 Here, we investigated the adsorption and desorption characteristics of DMPC from single bubbles, tens of micrometers in size, using an instrument based on an ultrasound transducer and a high-speed camera. The phospholipid, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), was purchased from NOF Corporation [CAS Registry No.…”

Section: Methodsmentioning

confidence: 99%

Adsorption and Desorption of a Phospholipid from Single Microbubbles under Pulsed Ultrasound Irradiation for Ultrasound-Triggered Drug Delivery

Nakata¹,

Tanimura²,

Koyama³

et al. 2019

Langmuir

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Microbubbles have potential for applications as drug and gene delivery systems in which the release of a substance is triggered by an ultrasonic pulse. In this paper, we discuss the adsorption and desorption of a film of phospholipid on the surface of a single microbubble under ultrasound irradiation. Our optical observation system consisted of a high-speed camera, a laser Doppler vibrometer, and an ultrasound cell; 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was used as the surfactant. The adsorption of the DMPC molecules onto the surface of the bubble was evaluated by measuring the contact angle between the bubble and a glass plate. A decrease of the contact angle of the bubble indicates desorption of the DMPC molecules from the bubble surface into the surrounding aqueous solution. The amount of DMPC molecules adsorbed on the bubble's surface is shown to decrease over time after bubble generation. The type and intensity of the pulsed ultrasound waves were varied so as mimic ultrasound triggered drug release. Increasing the number of cycles and the amplitude of the sound pressure of the pulsed ultrasound yielded a greater increase of the contact angle. We also measured the radial vibrations of the microbubbles in the ultrasound field. The vibrational characteristics of the microbubbles and the desorption characteristics of the DMPC molecules showed the same variation; namely, a greater sound pressure amplitude induced greater vibrational displacement and a larger amount of molecular desorption under resonance conditions. These results support the possibility of controlling drug release with pulsed ultrasound in a microbubble-based drug delivery system.

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“…The microscopic structure of the shell of a bubble and its rheology are the essential factors that determine bubble vibrational characteristics. A monolayer film of amphiphilic molecules is usually formed at the gas/liquid interface on the bubble surface. − Although the vibration amplitude of microbubbles coated with phospholipids or other surfactants under sonication is restrained by the viscoelasticity of the shell, the molecular film prevents the diffusion of the internal gas. Kabalnov et al , investigated the lifetime of fluorocarbon-stabilized phospholipid-coated microbubbles in the bloodstream theoretically and experimentally.…”

Section: Introductionmentioning

confidence: 99%

Vibration Characteristics and Persistence of Poloxamer- or Phospholipid-Coated Single Microbubbles under Ultrasound Irradiation

et al. 2019

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Microbubbles shelled with soft materials are expected to find applications as ultrasound-sensitive drug delivery systems, including through sonoporation. Microbubbles with specific vibrational characteristics and long intravascular persistence are required for clinical uses.To achieve this aim, the kinetics of the microbubble shell components at the gas/liquid interface while being subjected to ultrasound need to be better understood. This paper investigates the vibration characteristics and lifetime of single microbubbles coated with a poloxamer surfactant, Pluronic F-68, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) under ultrasound irradiation. Air-and perfluorohexane (PFH)-filled microbubbles coated with Pluronic F-68 and DMPC at several concentrations (0 to 10 −2 mol L −1 ) were produced. An optical measurement system using a laser Doppler vibrometer and microscope was used to observe the radial vibration mode of single microbubbles. The vibrational displacement amplitude and resonance radius of Pluronic-or DMPC-coated microbubbles were found to depend very little on the concentrations. The resonance radius was around 65 µm at 38.8 kHz under all the experimental conditions investigated. The lifetime of the microbubbles was investigated simultaneously by measuring their temporal change in volume, and it was increased with Pluronic concentration. Remarkably, the oscillation of the bubble has an effect on the bubble lifetime. In other words, the diffusion of the inner gas accelerates at resonance radius.

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Behavior of an Adsorbed Phospholipid Monolayer Submitted to Prolonged Periodical Surface Density Variations

Cited by 7 publications

References 25 publications

Adsorption of Phospholipids at the Air-Water Surface

Adsorption of Phospholipids at the Air-Water Surface

Adsorption and Desorption of a Phospholipid from Single Microbubbles under Pulsed Ultrasound Irradiation for Ultrasound-Triggered Drug Delivery

Vibration Characteristics and Persistence of Poloxamer- or Phospholipid-Coated Single Microbubbles under Ultrasound Irradiation

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