Behavior of Condensed Phosphates in Anion-Exchange Chromatography

Abstract

505tween actual composition and the experimental mean, however, are only -7.84% for the glutamic acid, +9.21% for lysine, and +8.24% for glycine.Analytical data on the amino acid composition of crystalline proteins such as d-lactoglobulin (Table II) and bovine serum albumin when determined by this method have been in good agreement with previously published (12, 13) values. In general, the procedures which have been described provide a less tedious, more rapid, and reasonably accurate approach to the analysis of protein hydrolyzates than has previously been available. ACKNOWLEDGMENTThe authors wish to acknowledge the technical assistance of John Rosevear in the early stages of this work and to express thanks to C. F. Marquardt for the statistical evaluations and also to Jean Hogan and Janet Dunlevv for photographic assistance.

Ionically Cross-Linked Poly(allylamine) as a Stimulus-Responsive Underwater Adhesive: Ionic Strength and pH Effects

“…PAH is a commercially available, weak polycation (effective pK a = 8.5 40 ) that, among its many other applications, is already used in its covalently cross-linked form as an oral drug for patients with chronic kidney disease. 41 Similarly, PPi (pK a,2 = 2.3, pK a,3 = 6.6, and pK a,4 = 9.3) and TPP (pK a,3 = 2.8, pK a,4 = 6.5, and pK a,5 = 9.2) are tetravalent and pentavalent polyprotic acids, 42 which are both on the U.S. Food and Drug Administration's generally recognized as safe (GRAS) list. Because of their biocompatibility, they are used (typically as sodium salts) as both food additives 43−45 and ionic cross-linkers for colloidal drug carriers.…”

Degradation of polyphosphates in solution. II. Hydrolysis of sodium hexaphosphate

“…The values of k~? , k64, 1~53, and k42 indicate that reaction (9) appears to be ncgligible. Similar results obtained with short- chain polyphosphates led Thilo** to doubt the occurrence of reaction (9) altogether.…”

“…, k64, 1~53, and k42 indicate that reaction (9) appears to be ncgligible. Similar results obtained with short- chain polyphosphates led Thilo** to doubt the occurrence of reaction (9) altogether. However, rate constants for reaction (9) determined on longchain polyphosphates, described in the following paper,Ig are of the order of 0.1 X 10W4 min-'.…”

Chromatography

“…can be obtained by dividing the resin bed height by the value of P. The column height required for a specific separation of two solutes can be approximated by [47]: This form of the equation is probably the easiest to calculate from experimental data.…”