Behavior of diglycerides and conjugated fatty acid triglycerides in reverse‐phase chromatography

Chang, Min; Conkerton, E. J.; Chapital, Dorselyn C.; Wan, Peter J.

doi:10.1007/bf02675915

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…Oils. Triglyceride content was determined by high-performance liquid chromatography (HPLC) as described by Chang et al (8). Fatty acid methyl esters were prepared and determined by gas chromatography according to Conkerton et al (9).…”

Section: Methodsmentioning

confidence: 99%

Chinese melon (Momordica charantia L.) seed: Composition and potential use

Chang

Conkerton

Chapital

et al. 1996

J Americ Oil Chem Soc

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Chinese melon (Momordica charantia L.), also known as bitter gourd, is a tropical crop, grown throughout Asian countries for use as food and medicinals. In 1993, four cultivars of Chinese melon were grown in Mississippi and the seeds were collected. Oil contents of the seeds ranged from 41 to 45% and the oils contained 63-68% eleostearic acid and 22-27% stearic acid. Industrially important tung oil, a "fast-drying oil" used in paints and varnishes, contains 90% eleostearic and 2-3% stearic acid. The ratio of stearic to eleostearic in Chinese melon seed oil is ten times greater than that in tung oil. The higher ratio should reduce the rate of drying and crosslinking and could be advantageous in the paint industry. The defatted meals contained 52-61% protein and would be a good source of methionine.

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Section: Methodsmentioning

confidence: 99%

Chinese melon (Momordica charantia L.) seed: Composition and potential use

Chang

Conkerton

Chapital

et al. 1996

J Americ Oil Chem Soc

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“…Seeds were excised and hand-shelled, and kernels were immediately frozen in liquid N 2 , then stored at -808C. Tung oil was extracted from kernels according to the method of Chang et al (1994) using a Tissuemizer (Tekmar). The ratio of kernel to petroleum ether was 1 g:10 mL.…”

Section: Plant Materials and Determination Of Seed Oil Contentmentioning

confidence: 99%

Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum

et al. 2006

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Seeds of the tung tree (Vernicia fordii) produce large quantities of triacylglycerols (TAGs) containing ;80% eleostearic acid, an unusual conjugated fatty acid. We present a comparative analysis of the genetic, functional, and cellular properties of tung type 1 and type 2 diacylglycerol acyltransferases (DGAT1 and DGAT2), two unrelated enzymes that catalyze the committed step in TAG biosynthesis. We show that both enzymes are encoded by single genes and that DGAT1 is expressed at similar levels in various organs, whereas DGAT2 is strongly induced in developing seeds at the onset of oil biosynthesis. Expression of DGAT1 and DGAT2 in yeast produced different types and proportions of TAGs containing eleostearic acid, with DGAT2 possessing an enhanced propensity for the synthesis of trieleostearin, the main component of tung oil. Both DGAT1 and DGAT2 are located in distinct, dynamic regions of the endoplasmic reticulum (ER), and surprisingly, these regions do not overlap. Furthermore, although both DGAT1 and DGAT2 contain a similar C-terminal pentapeptide ER retrieval motif, this motif alone is not sufficient for their localization to specific regions of the ER. These data suggest that DGAT1 and DGAT2 have nonredundant functions in plants and that the production of storage oils, including those containing unusual fatty acids, occurs in distinct ER subdomains.

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“…1a and b). The major peaks in the tung oil standard, in increasing elution times, are trieleostearin, linoleoyl-dieleostearin, a combination of oleoyl-dieleostearin and palmitoyl-dieleostearin, and stearoyl-dieleostearin (12) . Inspection of the UV spectrum from 250-350 nm indicated that the large unretained peak in Figure 1b contained very little eleostearic acid.…”

Section: Resultsmentioning

confidence: 99%

Differential extraction of eleostearic acid‐rich lipid‐protein complexes in tung seeds

Dyer¹,

Tang

Chapital³

et al. 1998

J Americ Oil Chem Soc

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Lipid-protein complexes were identified in the 104,000 × g supernatant fraction of developing tung seeds. Incubation of this fraction with linoleoyl-CoA promoted an increase of chloroform-extractable lipids in a time-dependent manner. High-performance liquid chromatography analysis indicated that the extracted lipids were similar to mature tung oil triglycerides. Differential extraction using chloroform or chloroform/methanol indicated that linoleoyl-CoA promoted extraction of pre-existing lipids rather than de novo synthesis. An increase in extractable lipids was also observed after incubation with proteinase K. Isolation of lipid-protein complexes by sucrose density centrifugation and analysis of proteins by gel electrophoresis revealed several proteins specifically associated with this lipid fraction. FIG. 1. High-performance liquid chromatography analysis of eleostearoyl lipids extracted after incubation of 104,000 × g supernatant with linoleoyl-CoA. (a) Tung oil standard. (b) Lipids extracted from the 104,000 × g supernatant after 20 min incubation. The three major peaks containing eleostearic acid are labeled 1, 2 and 3. (c) Changes in absorbance of each of the three major peaks observed in (b) throughout the course of the assay.

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Behavior of diglycerides and conjugated fatty acid triglycerides in reverse‐phase chromatography

Cited by 10 publications

References 15 publications

Chinese melon (Momordica charantia L.) seed: Composition and potential use

Chinese melon (Momordica charantia L.) seed: Composition and potential use

Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum

Differential extraction of eleostearic acid‐rich lipid‐protein complexes in tung seeds

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