Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP

Abstract

Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient’s own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β, collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highest in the i-PRF group. These findings demonstrate that liquid platelet concentrates can be formulated without the use of anticoagulants and present much translational potential for future research. Future animal and clinical trials are now necessary to further investigate the potential of utilizing i-PRF for soft tissue regenerative protocols in combination with various biomaterials.

Impacts of platelet‐rich fibrin and platelet‐rich plasma on primary osteoblast adhesion onto titanium implants in a bisphosphonate in vitro model

“…These findings indicate that osteoblasts cultured in PRF or PRP steered to a higher rate of adherence. These results are in accordance with those obtained in previous studies, which showed a positive effect of PRP and PRF on osseointegration of dental implants . Leucocyte‐enriched platelets derivatives appear to increase GF release of TGF‐beta1, VEGF, PDGF‐AB and improve angiogenesis, matrix production, hypercellularity and osseointegration .…”

“…There was no significant difference in the adhesion of osteoblasts to implant surfaces using PRP or PRF. This finding is consistent with those of Wang et al who compared the adhesion of osteoblasts to titanium implant surfaces using injectable‐PRF or PRP . Consequently, clinical use of those products may therefore improve wound healing after tooth extraction or decortication in BP patients via accumulation of growth factors and expression of extracellular matrix …”

Fluid platelet‐rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet‐rich plasma

“…Briefly, human skin fibroblasts were seeded in 24‐well plates at a density of 10 000 cells per well with 20% culture medium from PRP or fluid‐PRF. At 1 day, 3 days, and 5 days, cell numbers were determined using the Cell Counting Kit‐8 and measured at 450 nm using a microplate reader (PowerWave XS2, BioTek, Winooski) as previously described . The experiment was performed in triplicate with three independent experiments.…”

Telomere length and genetic variations affecting telomere length as biomarkers for facial regeneration with platelet‐rich fibrin based on the low‐speed centrifugation concept

“…As a result, autologous platelets products containing growth factors such as platelet‐derived growth factor (PDGF), transforming‐growth factor (TGF, especially TGF‐b), vascular endothelial growth factor (VEGF), insulin‐like growth factor (IGF), and epidermal growth factor (EGF), have been studied extensively over the last decade . Apart from their direct bulking effects, platelet concentrates promote angiogenesis and cause an enhanced production of collagen and fibronectin . They have been shown to retain their chemotactic and/or mitogenic action for various cells including monocytes, fibroblasts, stem cells (Figure ; Table ), smooth muscle cells, endothelial cells, and keratinocytes …”