Behavior of macrophage and osteoblast cell lines in contact with the β-TCP biomaterial (beta-tricalcium phosphate)

Arbez, Baptiste; Libouban, Hélène

doi:10.1016/j.morpho.2017.03.006

Cited by 25 publications

(12 citation statements)

References 29 publications

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“…Researchers should note that due to the unpredictable morphology of β-TCP nanoparticles after synthesis, it is not easy to envision the exact shape of these particles post-synthesis. The micro-Raman spectra (Figure 2) of our filler revealed characteristic peaks associated with β-TCP nanoparticles, similar to an earlier study conducted by Arbez et al [28].…”

Section: Discussionsupporting

confidence: 89%

Application of β-Tricalcium Phosphate in Adhesive Dentin Bonding

et al. 2021

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The study aimed at synthesizing β-tricalcium phosphate (β-TCP) nanoparticles and comparing the mechanical properties and dentin interaction of two adhesives: experimental adhesive (EA) and EA with 5 wt.% β-TCP nanoparticles (β-TCP-5%). These filler nanoparticles were synthesized and then characterized with scanning electron microscopy (SEM) and micro-Raman spectroscopy. The β-TCP nanoparticles were incorporated in the adhesives to form two groups: gp-1: EA (control) and gp-2: β-TCP-5%. These adhesives were characterized by SEM, energy-dispersive X-ray (EDX) spectroscopy and were also assessed for their micro-tensile bond strength (μTBS) with (TC) and without thermocycling (NTC). Fourier Transform Infrared (FTIR) spectroscopy was performed to evaluate the degree of conversion (DC) of two adhesives. The β-TCP filler was seen as irregularly shaped agglomerates on SEM. The micro-Raman spectra revealed characteristic peaks associated with β-TCP nanoparticles. Both adhesives presented suitable dentin interaction, which was demonstrated by the formation of resin tags of variable depths. The EDX analysis verified the existence of calcium (Ca) and phosphate (P) for the β-TCP-5% group. The greatest μTBS values were shown by β-TCP-5% group samples when they were non-thermocycled (NTC) (β-TCP-5%-NTC: 34.11 ± 3.46) followed by the thermocycled (TC) samples of the same group (β-TCP-5%-TC: 30.38 ± 3.66), compared with the EA group. Although the DC presented by β-TCP-5% group was comparable to the EA group, it was still lower. The addition of β-TCP nanoparticles in the adhesive improved its μTBS and resulted in a suitable dentin interaction, seen in the form of hybrid layer and resin tag formation. Nonetheless, a decreased DC was observed for the β-TCP-5% adhesive. Future studies probing the effect of different filler concentrations on various properties of the adhesive are warranted.

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Section: Discussionsupporting

confidence: 89%

Application of β-Tricalcium Phosphate in Adhesive Dentin Bonding

et al. 2021

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“…As explained earlier, calcium and phosphorus are part of the composition of the tooth [ 30 ], and they can remineralize the tooth instantaneously after acidic challenge [ 26 ]. The FTIR spectra ( Figure 2 A) displayed characteristic bands for β-TCP nanoparticles, and the micro-Raman spectra ( Figure 2 B) also displayed characteristic peaks related to these filler nanoparticles, similar to several previous studies [ 21 , 30 , 31 ].…”

Section: Discussionsupporting

confidence: 86%

Influence of Concentration Levels of β-Tricalcium Phosphate on the Physical Properties of a Dental Adhesive

et al. 2022

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Our study assessed the influence of integrating 5% and 10% tricalcium phosphate (β-TCP-Ca3(PO4)2.) nanoparticles into a dental adhesive on the adhesive’s bonding. To evaluate the filler nanoparticles, scanning electron microscopy (SEM), Energy Dispersive X-Ray (EDX) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and micro-Raman spectroscopy techniques were used. Shear Bond strength (SBS) testing, degree of conversion (DC) analysis, investigation of the adhesive–dentin interface, and biofilm experiments were conducted. The SEM micrographs revealed non-uniform agglomerates, while the EDX demonstrated the existence of oxygen ‘O’ (24.2%), phosphorus ‘P’ (17.4%) and calcium ‘Ca’ (60.1%) in the β-TCP nanoparticles. The FTIR and micro-Raman spectra indicated characteristic bands for β-TCP containing materials. The 10 wt.% β-TCP adhesive presented the highest SBS values (NTC-10 wt.% β-TCP: 33.55 ± 3.73 MPa, TC-10 wt.% β-TCP: 30.50 ± 3.25 MPa), followed by the 5 wt.% β-TCP adhesive (NTC-5 wt.% β-TCP: 32.37 ± 3.10 MPa, TC-5 wt.% β-TCP: 27.75 ± 3.15 MPa). Most of the detected failures after bond strength testing were adhesive in nature. The β-TCP adhesives demonstrated suitable dentin interaction by forming a hybrid layer (with few or no gaps) and resin tags. The β-TCP adhesives (10 wt.%) revealed lower DC values compared to control. The incorporation of 5 and 10 wt.% concentrations of β-TCP particles resulted in an increase in SBS values. A linear decline in DC values was witnessed when the nanoparticle concentration was increased. Further research focusing on exploring the influence of higher filler concentrations on adhesive’s properties is recommended.

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“…Terranova et al showed that β‐TCP fine particles coupled with polymer fibers induced a stimulatory effect on osteoblasts to promote bone formation of mouse (Terranova et al, 2017). In general, it is considered that β‐TCP is phagocytized by macrophages and osteoclasts and provides Ca/P ions to regenerative regions (Arbez & Libouban, 2017; Arbez, Manero, Mabilleau, Libouban, & Chappard, 2019; Detsch et al, 2010). In immunostaining assessments, CD68‐positive macrophages frequently appeared on the surface of RCP/β‐TCP, but not on the surface of newly formed bone (Figure 6), suggesting that RCP/β‐TCP are degraded by macrophages.…”

Section: Discussionmentioning

confidence: 99%

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

et al. 2020

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Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl‐glycyl‐aspartic acid (RGD)‐rich motif to enhance cell behavior and is anticipated as a xeno‐free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta‐tricalcium phosphate fine particles (RCP/β‐TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β‐TCP. Recombinant peptide was thermal crosslinked and freeze‐dried to prepare RCP. An aqueous dispersion of β‐TCP fine particles was added to RCP to obtain RCP/β‐TCP. Subsequently, RCP/β‐TCP were characterized using scanning electron microscopy (SEM), energy dispersive X‐ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β‐TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β‐TCP, β‐TCP particles dose‐dependently covered the surface of RCP. Cell culture tests showed that RCP/β‐TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3‐E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β‐TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β‐TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.

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Behavior of macrophage and osteoblast cell lines in contact with the β-TCP biomaterial (beta-tricalcium phosphate)

Cited by 25 publications

References 29 publications

Application of β-Tricalcium Phosphate in Adhesive Dentin Bonding

Application of β-Tricalcium Phosphate in Adhesive Dentin Bonding

Influence of Concentration Levels of β-Tricalcium Phosphate on the Physical Properties of a Dental Adhesive

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

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