Behavioral effects of essential oil of Citrus aurantium L. inhalation in rats

Abstract

RESUMO: "Efeitos comportamentais em ratos após inalação do óleo essencial de Citrus aurantium L.". O óleo essencial de laranja (OEL) e seus constituintes obtidos da Citrus aurantium L. (Rutaceae) têm despertado interesse pela sua ação sedativa e relaxante. No presente estudo, ratos previamente expostos à inalação do OEL nas concentrações de 1,0%; 2,5% e 5,0%, p/v, durante 7 minutos em caixas de acrílico, foram avaliados em dois modelos de ansiedade: labirinto em cruz elevado (LCE) e campo aberto. O OEL na concentração de 2,5% aumentou tanto o tempo de permanência dos animais nos braços abertos do LCE, como o tempo de interação social ativa no campo aberto, tendo sido superior ao grupo padrão diazepam (1,5 mg/kg) ip. A diminuição do grau de emocionalidade dos animais observada nos dois modelos experimentais sugere uma possível ação central, o que está de acordo com o perfi l fi toquímico do óleo em estudo o qual mostrou a presença de limoneno (96,24%) e mirceno (2,24%), constituintes com comprovada atividade depressora sobre o sistema nervoso central. Unitermos:Óleo essencial, Citrus aurantium, Rutaceae, óleo de laranja, ansiedade, inalação, labirinto em cruz elevado. ABSTRACT:The orange essential oil (OEO) and its components obtained from Citrus aurantium L. (Rutaceae) has been attracting interest due to its sedative and relaxing actions. In the present study, rats previously exposed to OEO at the concentrations of 1.0%; 2.5% and 5.0%, w/w, by inhalation during 7 minutes in acrylic boxes were evaluated in two anxiety models: elevated plus maze (EPM) and open-fi eld. The OEO at the concentration of 2.5% increased both the time of the animals in the open arms of the EPM and the time of active social interaction in the open-fi eld being longer than that of the diazepam group (1.5 mg/kg i.p). In conclusion, the decrease in the level of emotionality of the animals observed in the two experimental models suggests a possible central action, which is in agreement with the phytochemical profi le of the oil under study, since it showed the presence of limonene (96.24%) and mircene (2.24%), components with a wellknown depressant activity on the central nervous system.

Bioassay-guided evaluation of central nervous system effects of citronellal in rodents

“…CT at all doses caused a significant reduction of ambulation of animals in the test of spontaneous movement after 0.5, 1 and 1.5 h of its administration in the doses of 100, 200 and 400 mg/kg, that corroborates with the hypothesis of the CT reduces the CNS activity. It was reported that reduction of the ambulation of the animals is characteristic of psychopharmacological drugs (Leite et al, 2008;Carlini, 2003).…”

Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish ( Rhamdia quelen )

“…The most noteworthy among these are C. x sinensis (Faturi et al, 2010) and C. x limon (Ceccarelli et al, 2004) in rats, and C. x latifolia (Gargano et al, 2008) and C. x aurantium (Carvalho-Freitas, Costa, 2002;Pultrini et al, 2006) in mice. Sedative and anxiolytic-like effects have been described for the EO obtained from the peel of Citrus x aurantium in rats and mice (Leite et al, 2008;Costa et al, 2013). However, the literature does not present reports of their anesthetic effects on fish.…”

Evaluation of effects of dichloromethane fraction from Platonia insignis on pilocarpine-induced seizures

“…Our data suggest that DMF may be a stimulatory to the CNS, since studies shows that reduction of the ambulation of the animals is characteristic of depressant drugs (Carlini, 2003;Freire et al, 2006;Leite et al, 2008;Quintans Júnior et al, 2008b).…”