Behavioral outcomes of a novel, pelvic nerve damage rat model of fecal incontinence

Janssen, P. T. J.; Breukink, Stéphanie O.; Melenhorst, Jarno; Stassen, Laurents P. S.; Bouvy, Nicole D.; Temel, Yasin; Jahanshahi, Ali

doi:10.1111/nmo.13242

Cited by 1 publication

(6 citation statements)

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“…The VD model has the advantage of being more closely related to natural birth as compared with the retro-uterine balloon inflation model 4 or the transvaginal retro-uterine intrapelvic balloon inflation model. 5 The responder rate following the 2-hour vaginal balloon inflation (29%) is comparable to the responder rate of the retro-uterine balloon inflation model (32%) as described by Devane et al 4 Unfortunately, the responder rate is relatively low, which makes the animal experiments, from an ethical point of view, rather complicated. Interestingly, the level of incontinence in responders following the 2-hour vaginal balloon inflation (DBI = 0.65 ± 0.12) seems higher as compared with the retro-uterine balloon inflation model.…”

Section: Discussionmentioning

confidence: 58%

“…These studies utilized either retro-uterine balloon inflation 4 or transvaginal retro-uterine intrapelvic balloon inflation. 5 While both balloon-inflation models showed signs of fecal incontinence, the responder rate was limited; for instance, with the retro-uterine balloon inflation model, it did not exceed 32% of animals. 4 The retro-uterine intrapelvic balloon inflation model resulted in a small effect on the behavioral outcome, and this very small window and this did not enable the analysis of future treatment effects.…”

Section: Introductionmentioning

confidence: 97%

“…4 The retrouterine intrapelvic balloon inflation model resulted in a small effect on the behavioral outcome, and this very small window and this did not enable the analysis of future treatment effects. 5 Currently, the vaginal distention (VD) model is commonly used for stress urinary incontinence. 6,7 This model is characterized by intravaginal balloon inflation, which mimics the compressive forces on the pelvic floor during the second phase of natural delivery.…”

Section: Introductionmentioning

confidence: 99%

“…Fecal and stress urinary incontinence are a possible consequence of natural birth due to excessive compressive forces on the pelvic floor that cause damage to it. [1][2][3] Presently, the preclinical research 4,5 into the treatment and mechanisms of action (MoAs) of fecal incontinence is limited, and only two preclinical models related to defecation behavior have been published in literature to date. These studies utilized either retro-uterine balloon inflation 4 or transvaginal retro-uterine intrapelvic balloon inflation.…”

Section: Introductionmentioning

confidence: 99%

“…4 The retro-uterine intrapelvic balloon inflation model resulted in a small effect on the behavioral outcome, and this very small window and this did not enable the analysis of future treatment effects. 5…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Vaginal Distention Rodent Model for Fecal Incontinence: A Pilot Study on the Effect on Defecation Behavior

Douven

Franken

Debets

et al. 2022

Journal of Coloproctology

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Objectives Vaginal balloon inflation simulates the compressive forces on the pelvic floor during the second phase of natural delivery. The foremost use of this animal model of vaginal distention (VD) is to study the mechanisms underlying urinary incontinence. As damage to the pelvic floor during natural birth is a common cause of fecal incontinence, the present paper aimed to investigate the effect of VD on defecation behavior in adult rats. Methods Vaginal distention was performed in 8 rats for 2 hours, and in 3 rats for 4 hours, and sham inflation was performed in 4 rats. With the use of a latrine box in the rat home-cage and 24/7 video tracking, the defecation behavior was examined. The time spent in and outside the latrine was monitored for two weeks preoperatively and three weeks postoperatively, and a defecation behavior index (DBI; range: 0 [continent] to 1 [incontinent]) was defined. Pelvic floor tissue was collected postmortem and stained with hematoxylin and eosin. Results Vaginal balloon inflation for 2 hours resulted in fecal incontinence in 29% of the animals (responders) whereas the DBI scores of non-responders (71%) and control animals did not change in the postoperative phase compared with the baseline score. A 4-hour balloon inflation resulted in fecal incontinence in 1 animal and caused a humane endpoint in 2 animals with markedly more tissue damage in the 4-hour responder compared with the 2-hour responders. Conclusions Vaginal balloon inflation, with an optimum duration between 2 and 4 hours, can be used as a model to study changes in defecation behavior in rats induced by pelvic floor damage.

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Section: Discussionmentioning

confidence: 58%