Behaviour of L. monocytogenes in an artificially made biofilm of a nisin-producing strain of Lactococcus lactis

Leriche, V.; Chassaing, Danielle; Carpentier, Brigitte

doi:10.1016/s0168-1605(99)00128-2

Cited by 62 publications

(42 citation statements)

References 41 publications

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“…It was chosen because its restriction fragment length polymorphism profile was the most representative of L. monocytogenes strains found in dairy premises (C. Martiré, personal communication). This L. monocytogenes strain, a 4b serotype, has already been used in several previous studies (5,8,27,29,35). Cultures were maintained for no more than 1 month on tryptone soya agar slopes (TSA; Difco, Le Pont de Claix, France) at 3°C.…”

Section: Methodsmentioning

confidence: 99%

Construction and Analysis of Fractional Multifactorial Designs To Study Attachment Strength and Transfer of Listeria monocytogenes from Pure or Mixed Biofilms after Contact with a Solid Model Food

Midelet

Kobilinsky

Carpentier

2006

Appl Environ Microbiol

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The aim of this study was to establish which of seven factors influence the adhesion strength and hence bacterial transfer between biofilms containing Listeria monocytogenes (pure and two-species biofilms) and tryptone soya agar (TSA) as a solid organic surface. The two-species biofilms were made of L. monocytogenes and one of the following species of bacteria: the nonpathogenic organisms Kocuria varians, Pseudomonas fluorescens, and Staphylococcus sciuri and CCL 63, an unidentified gram-negative bacterium isolated from the processing plant environment. We used biofilms prepared under conditions simulating open surfaces in meat-processing sites. The biofilm's adhesion strength and population were evaluated by making 12 contacts on a given whole biofilm (4.5 cm 2 ), using a new slice of a sterilized TSA cylinder for each contact, and plotting the logarithm CFU · cm ؊2 detached by each contact against the contact number. Three types of detachment kinetics were observed: biphasic kinetics, where the first slope may be either positive or negative, and monophasic kinetics. The bacteria that resisted a chlorinated alkaline product and a glutaraldehyde-and quaternary ammonium-based disinfectant had greater adhesion strengths than those determined for untreated biofilms. One of the four non-Listeria strains studied, Kocuria varians CCL 56, favored both the attachment and detachment of L. monocytogenes. The stainless steel had smaller bacterial populations than polymer materials, and non-Listeria bacteria adhered to it less strongly. Our results helped to evaluate measures aimed at controlling the immediate risk, linked to the presence of a large number of CFU in a foodstuff, and the delayed risk, linked to the persistence of L. monocytogenes and the occurrence of slightly contaminated foods that may become dangerous if L. monocytogenes multiplies during storage. Cleaning and disinfection reduce the immediate risk, while reducing the delayed risk should be achieved by lowering the adhesion strength, which the sanitizers used here cannot do at low concentrations.Microorganisms in foods may be derived from the raw materials, ingredients, personnel, or the work environment. Epidemics of listeriosis, which is rare but serious when it affects the so-called at-risk population (43), have been traced back to environmental contamination of food processing areas (48). Listeria monocytogenes genotypes, absent from the raw materials, may be repeatedly found in the finished products and on work surfaces (3). It is therefore interesting to identify the factors that affect adhesion strength and hence transfer of L. monocytogenes from an inert surface to a food following contact. Such data can be useful for quantitative analysis of the risks associated with ready-to-eat foods that may be contaminated during manufacture (11,18). Most studies of detachment of microbial cells use biofilms formed under dynamic conditions in which detachment is induced by liquid flow. These studies have shown the influence of numerous factors on the detachme...

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Section: Methodsmentioning

confidence: 99%

Construction and Analysis of Fractional Multifactorial Designs To Study Attachment Strength and Transfer of Listeria monocytogenes from Pure or Mixed Biofilms after Contact with a Solid Model Food

Midelet

Kobilinsky

Carpentier

2006

Appl Environ Microbiol

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“…Les biofilms « positifs » sont des biofilms protecteurs susceptibles de modifier les propriétés physico-chimiques des substrats [6], avec pour conséquence positive, une alté-ration de l'implantation de micro-organismes planctoniques indésirables entrant à leur contact. Les biofilms « positifs » de lactocoques producteurs de bactériocine ont été employés avec succès au laboratoire [14]. En outre, une large gamme de produits fondée sur les propriétés de l'association Bacillus et Lactobacillus est commercialisée pour le traitement des lisiers, des litières, la réduction des odeurs et la prévention contre les bactéries pathogènes (www.cobiotex.fr).…”

Section: Introductionunclassified

Article

Ibrahim

Briandet²,

Mistou

et al. 2004

Lait

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-Immobilization of lactococci. Bacteria present in dairy products exist predominantly in an immobilized form, as coagulated milk is not a liquid and thus does not allow bacteria to move free. Lactococci may be immobilized by attachment to surfaces, chain formation and by trapping in the extracellular matrix. Use of semi-liquid medium to simulate immobilized growth revealed characteristic properties of non-planctonic lactococcal cultures, which are: limited distribution in the medium, slow growth, a mixture of the stationary and exponentially growing cells, and genetic instability of the culture. These characteristics depend on molecular composition of the cell wall. We have shown that peptidoglycan (PG) breaks, which can be introduced not only by PG hydrolases, but also by defects of PG synthesis machinery (mutation in the ponA gene) play a significant role in immobilization (adhesion, sedimentation, chain formation). We also showed that cell wall anchored proteins play a positive role in L. lactis adhesion. Our studies contribute to the identification of molecular determinants responsible for the phenomenon of adhesion, formation of biofilms, and more generally for functioning of the lactic acid bacteria in natural and industrial environments. They have equally as objective to define the molecular factors necessary for the establishment of positive biofilms, which would minimize the risks of contamination in dairy industry. Lactococcus lactis / adhesion / biofilm / cell wall / peptidoglycan hydrolaseRésumé -Dans l'industrie laitière, les bactéries se retrouvent la plupart du temps sous une forme immobilisée, dans la mesure où le lait coagulé ne constitue pas un milieu liquide et ne permet donc pas aux bactéries en croissance des déplacements libres. Notre principal objectif réside dans la modélisation de la croissance immobilisée. Les lactocoques peuvent être immobilisés par un attachement sur les surfaces, la formation de chaînes ou l'emprisonnement au sein d'une matrice extra cellulaire. L'utilisation d'un milieu semi-liquide comme modèle d'immobilisation nous a permis de montrer que les propriétés caractéristiques de la croissance non-planctonique des lactocoques consistent en une distribution limitée dans le milieu, une croissance lente et le mélange de cellules en phases stationnaires et exponentielles, et que l'immobilisation est à l'origine de l'instabilité génétique de la culture. Ces caractéristiques dépendent de la composition moléculaire de la paroi cellulaire. Les cassures de peptidoglycane (PG), qui peuvent être introduites non seulement par des PG hydrolases, mais aussi suite à des défauts de la machinerie de synthèse du PG (mutation du gène ponA), sont importantes dans la croissance sous forme immobilisée des * Auteur correspondant : Saulius.Kulakauskas@diamant.jouy.inra.fr 104 M. Ibrahim et al.

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“…Evidently, bacterial pathogens, such as L. monocytogenes and S. enterica, can be entrapped in multispecies sessile communities formed on such surfaces. In addition, attachment and biofilm formation by these two species have been shown to be influenced by either the natural in situ presence of other species or just their metabolic by-products (8,10,11,24,26,27,29,32,33,37,38,39,46,58,77). For instance, the presence of Staphylococcus xylosus and Pseudomonas fragi affected the numbers of L. monocytogenes biofilm cells on stainless steel (51), while compounds present in Hafnia alvei cell-free culture supernatant inhibited the early stage of S. enterica serovar Enteritidis biofilm formation on the same material (10).…”

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confidence: 99%

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

Kostaki

Chorianopoulos

Braxou

et al. 2012

Appl Environ Microbiol

152

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ABSTRACTThis study aimed to investigate the possible influence of bacterial intra- and interspecies interactions on the ability ofListeria monocytogenesandSalmonella entericato develop mixed-culture biofilms on an abiotic substratum, as well as on the subsequent resistance of sessile cells to chemical disinfection. Initially, three strains from each species were selected and left to attach and form biofilms on stainless steel (SS) coupons incubated at 15°C for 144 h, in periodically renewable tryptone soy broth (TSB), under either monoculture or mixed-culture (mono-/dual-species) conditions. Following biofilm formation, mixed-culture sessile communities were subjected to 6-min disinfection treatments with (i) benzalkonium chloride (50 ppm), (ii) sodium hypochlorite (10 ppm), (iii) peracetic acid (10 ppm), and (iv) a mixture of hydrogen peroxide (5 ppm) and peracetic acid (5 ppm). Results revealed that both species reached similar biofilm counts (ca. 105CFU cm−2) and that, in general, interspecies interactions did not have any significant effect either on the biofilm-forming ability (as this was assessed by agar plating enumeration of the mechanically detached biofilm bacteria) or on the antimicrobial resistance of each individual species. Interestingly, pulsed-field gel electrophoresis (PFGE) analysis clearly showed that the threeL. monocytogenesstrains did not contribute at the same level either to the formation of mixed-culture sessile communities (mono-/dual species) or to their antimicrobial recalcitrance. Additionally, the simultaneous existence inside the biofilm structure ofS. entericacells seemed to influence the occurrence and resistance pattern ofL. monocytogenesstrains. In sum, this study highlights the impact of microbial interactions taking place inside a mixed-culture sessile community on both its population dynamics and disinfection resistance.

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Behaviour of L. monocytogenes in an artificially made biofilm of a nisin-producing strain of Lactococcus lactis

Cited by 62 publications

References 41 publications

Construction and Analysis of Fractional Multifactorial Designs To Study Attachment Strength and Transfer of Listeria monocytogenes from Pure or Mixed Biofilms after Contact with a Solid Model Food

Construction and Analysis of Fractional Multifactorial Designs To Study Attachment Strength and Transfer of Listeria monocytogenes from Pure or Mixed Biofilms after Contact with a Solid Model Food

Article

Differential Biofilm Formation and Chemical Disinfection Resistance of Sessile Cells of Listeria monocytogenes Strains under Monospecies and Dual-Species (with Salmonella enterica) Conditions

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