Behaviour of Two Isolates of Plum Pox Virus Inoculated on Peach and Apricot Trees: First Results

Quiot, J. B.; Labonne, Gérard; Boeglin, M.; Adamolle, C.; Renaud, L.Y.; Candresse, Thierry

doi:10.17660/actahortic.1995.386.39

Cited by 38 publications

(24 citation statements)

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“…However, these are broad discriminations that may not apply to some specific isolates or to other epidemiological conditions. Such differences have been best described in France and Italy, where the introduction of isolates belonging to the PPV‐M strain resulted in the unusually rapid spread of infection in peach orchards (Quiot et al ., 1995; Bianco et al ., 2004). Further analysis, in France, of the prevalence of isolates belonging to the PPV‐D or PPV‐M strains in peach and apricot indicated that PPV‐D isolates were predominantly restricted to apricot orchards whereas PPV‐M isolates were common in peach orchards (Dallot et al ., 1998).…”

Section: Biological and Epidemiological Properties Of Ppv Strainsmentioning

confidence: 99%

Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains

Candresse¹,

Cambra²

2006

EPPO Bulletin

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Knowledge of the existence of strains or types of Plum pox virus (PPV), defined as monophyletic groups of closely related isolates sharing similar properties, has gradually emerged. Early attempts illustrated both the interest and the limits of biological properties for discrimination of isolates but in the late 1970s gel‐immunodiffusion assays allowed the grouping of PPV isolates into two serogroups typified by the Dideron (D) and Marcus (M) isolates. Technical difficulties, however, limited the widespread use of this approach and only in the early 1990s, with the availability of more discriminating and higher throughput techniques, did it become clear that PPV isolates could be allocated into two major groups. This led to the description of the strains, or types, PPV‐D and PPV‐M. Following this, distant PPV isolates typifying other strains such as El Amar (PPV‐EA), Cherry (PPV‐C) or Winona (PPV‐W) were identified, as well as the widespread group of recombinants known as PPV‐Rec. Each of the six known strains or types can now be identified using a range of serological or molecular techniques. However, their evolutionary history and, in particular, the reason for the wide prevalence of the PPV‐D, ‐M and ‐Rec types relative to PPV‐EA, ‐C or ‐W is still a matter of speculation.

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Section: Biological and Epidemiological Properties Of Ppv Strainsmentioning

confidence: 99%

Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains

Candresse¹,

Cambra²

2006

EPPO Bulletin

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“…Both methods of evaluation confirmed that Marcus isolates are more virulent than Dideron isolates (Quiot et al, 1995;Cambra et al, 2006b). Focusing on our objective, the least susceptible rootstock against both Dideron and Marcus isolates was GF677 (almond × peach).…”

Section: Discussionmentioning

confidence: 59%

Susceptibility of Prunus rootstocks against Marcus and Dideron isolates of Plum pox virus by graft‐inoculation

Rubio

Dicenta

Masse

et al. 2013

Annals of Applied Biology

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Sharka (Plum pox virus, PPV) is one of the most important diseases affecting stone fruits. While there is much information on the reaction of cultivars to PPV infection, details about rootstocks are scarce. In this study, we evaluated 28 stone fruit rootstocks belonging to different Prunus species against the Marcus and Dideron strains of PPV. Rootstocks were evaluated under controlled conditions during two growing seasons using two inoculation methods: direct inoculation of own-rooted rootstocks and grafting onto PPV-infected GF305 peach seedlings. Our results showed a generalised susceptibility of the rootstocks evaluated. Evaluating own-rooted rootstocks was more efficient than the traditional method of grafting onto infected GF305 seedlings. As expected, PPV-M was found to be more aggressive than PPV-D, producing symptoms and occurring in a higher number of plants, as shown by ELISA. The most susceptible rootstocks were Myran, Viking, Myro pg Pecher, Julior, Myrobolan 29C, Rubira, Myrobolan B, MrS 2/5, Jaspi and MP8. The least susceptible were GF677, Myrotop, Citation and ZH6. These results highlight the necessity to breed PPV-resistant rootstocks for the different stone fruit species.

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“…Plum pox virus (PPV) was first detected in France in the 1960s (Morvan, 1988), especially on apricot trees, where the disease was maintained at a very low incidence by control measures. A new phase in sharka epidemics began in the 1990s with the detection of infections which spread at an unusual rapidity in peach orchards (Quiot et al ., 1995).…”

Section: Ppv Diversitymentioning

confidence: 99%

“…The distribution of PPV‐D and PPV‐M isolates was clearly unequal on apricot and peach trees: 80% of PPV‐M isolates being found on peach trees, whereas on apricot trees PPV‐D isolates predominated although PPV‐M isolates were also found (Dallot et al ., 1998). It is believed that such an uneven distribution reflects distinct epidemic potentialities of PPV strains as well as the historical background of sharka disease in France (Quiot et al ., 1995).…”

Section: Ppv Diversitymentioning

confidence: 99%

Epidemiology of sharka disease in France

Labonne¹,

Dallot²

2006

EPPO Bulletin

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Plum pox virus was first detected in France in the 1960s. Both PPV-D and PPV-M strains are present but epidemics related to the PPV-M strain detected in the late 1980s are the most problematic. The two PPV strains have unequal distributions in peach and apricot orchards and different prevalences. More than 20 different aphid species have been identified as vectors of PPV but most of them do not colonize Prunus species. Thus, aphids involved in the spread of PPV in orchards are essentially visiting aphids. The main sources of inoculum for the vectors are leaves and fruits of infected stone-fruit trees. Spontaneous, wild and ornamental Prunus species such as Prunus dulcis , P. spinosa or P. pissardii are susceptible to PPV isolates found in France but their role as a reservoir in sharka epidemics is probably negligible. The disease spreads rapidly in orchards but the rate of progression may vary according to the identity of the PPV strain and the Prunus species. Analysis of spatial patterns of disease has shown that secondary spread by aphids frequently occurs over short distances in the orchards (aggregated patterns) but also that dissemination at longer distances (of several hundred metres) is a common event. PPV DiversityPlum pox virus (PPV) was first detected in France in the 1960s (Morvan, 1988), especially on apricot trees, where the disease was maintained at a very low incidence by control measures. A new phase in sharka epidemics began in the 1990s with the detection of infections which spread at an unusual rapidity in peach orchards .A survey was carried out in apricot and peach orchards in the years 1992-95 by the French Plant Protection Service jointly with INRA to characterize the diversity of PPV populations present in south-eastern France. 200 isolates were collected and characterised using electro-blot immunoassay (EBIA) and Restriction Fragment Length Polymorphism (RFLP) analysis targeting the coat protein gene. Sequencing of the 5 ′ -and 3 ′ ends of the CP gene was also carried out for a selected set of isolates (Dallot et al ., 1998). The majority of the collected isolates belonged to the two main PPV groups (PPV-D and PPV-M strains). A minority of isolates (10%) collected on peach trees showed an atypical electrophoretic mobility pattern but were classified as the PPV-D strain based on sequence properties. A more limited screening of PPV isolates was performed later on but did not provide evidence of the presence of strains of PPV other than PPV-M and PPV-D in France, nor of recombinant isolates Glasa et al ., 2002).The distribution of PPV-D and PPV-M isolates was clearly unequal on apricot and peach trees: 80% of PPV-M isolates being found on peach trees, whereas on apricot trees PPV-D isolates predominated although PPV-M isolates were also found (Dallot et al ., 1998). It is believed that such an uneven distribution reflects distinct epidemic potentialities of PPV strains as well as the historical background of sharka disease in France . Aphid speciesPPV is a non-persistently transmitted aphid-...

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Behaviour of Two Isolates of Plum Pox Virus Inoculated on Peach and Apricot Trees: First Results

Cited by 38 publications

References 0 publications

Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains

Causal agent of sharka disease: historical perspective and current status of Plum pox virus strains

Susceptibility of Prunus rootstocks against Marcus and Dideron isolates of Plum pox virus by graft‐inoculation

Epidemiology of sharka disease in France

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