Susceptibility of Prunus rootstocks against Marcus and Dideron isolates of Plum pox virus by graft‐inoculation

Abstract

Sharka (Plum pox virus, PPV) is one of the most important diseases affecting stone fruits. While there is much information on the reaction of cultivars to PPV infection, details about rootstocks are scarce. In this study, we evaluated 28 stone fruit rootstocks belonging to different Prunus species against the Marcus and Dideron strains of PPV. Rootstocks were evaluated under controlled conditions during two growing seasons using two inoculation methods: direct inoculation of own-rooted rootstocks and grafting onto PPV-infected GF305 peach seedlings. Our results showed a generalised susceptibility of the rootstocks evaluated. Evaluating own-rooted rootstocks was more efficient than the traditional method of grafting onto infected GF305 seedlings. As expected, PPV-M was found to be more aggressive than PPV-D, producing symptoms and occurring in a higher number of plants, as shown by ELISA. The most susceptible rootstocks were Myran, Viking, Myro pg Pecher, Julior, Myrobolan 29C, Rubira, Myrobolan B, MrS 2/5, Jaspi and MP8. The least susceptible were GF677, Myrotop, Citation and ZH6. These results highlight the necessity to breed PPV-resistant rootstocks for the different stone fruit species.

Evaluation of Turkish apricot germplasm using SSR markers: Genetic diversity assessment and search for Plum pox virus resistance alleles

“…The major producer country is Turkey, followed by Iran, Uzbekistan, Algeria and Italy. The one of the most serious problem of apricot is the Sharka disease, which is caused by Plum pox virus (PPV) (Rubio et al, 2013). The fruits of infected apricots can exhibit early ripening and are misshapened resulting in losses in yields and marketability.…”

Sharka: how do plants respond to Plum pox virus infection?

“…The incorporation of resistance genes from other related wild species has also become a useful alternative. For example, the induction of resistance to PPV in "GF305" peach by "Garrigues" almond grafting has recently been reported (Rubio et al, 2013), suggesting that "Garrigues" grafting could be used as a natural vaccine against PPV in peach, although more studies are necessary to identify the resistance factors and understand how they work (Rubio et al, 2013).…”

Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees

“…PPV is transmitted in the field by many aphid species, such as Aphis spiraecola , Aphis gossypii , Myzus persicae , Phorodon humuli , and Hyalopterus pruni , in a non-circulative manner [ 7 , 8 , 9 ]. PPV can also be transmitted through grafting in nurseries [ 10 , 11 ]. Therefore, transport of infected plant materials and tree seedings from one location to another is often considered as the main route of PPV long-distance spread, including the spread between two different countries.…”