Behavioural analysis of the efficacy of treatments for injuries to the spinal cord in animals

Webb, Aubrey A.; Muir, Gillian D.; Jeffrey, N.D.; Olby, Natasha J.

doi:10.1136/vr.155.8.225

Cited by 27 publications

(16 citation statements)

References 71 publications

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“…Olby et al modified the BBB open field scoring system for dogs based on the pelvic limbs [24]. Several investigators have confirmed the reliability of the Olby scoring system [23,38]. The spinal cord injury model in the present study had more than 75% of the spinal canal occluded during a 12 h period; the resulting lesions showed histopathologically severe hemorrhage and vacuolar formation.…”

Section: Discussionsupporting

confidence: 57%

Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs

et al. 2007

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This study was to determine the effects of allogenic umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) and recombinant methionyl human granulocyte colony-stimulating factor (rmhGCSF) on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-five adult mongrel dogs were assigned to five groups according to treatment after a spinal cord injury: no treatment (CN); saline treatment (CP); rmhGCSF treatment (G); UCB-MSCs treatment (UCB-MSC); co-treatment (UCBG). The UCB-MSCs isolated from cord blood of canine fetuses were prepared as 106 cells/150 µl saline. The UCB-MSCs were directly injected into the injured site of the spinal cord and rmhGCSF was administered subcutaneously 1 week after the induction of spinal cord injury. The Olby score, magnetic resonance imaging, somatosensory evoked potentials and histopathological examinations were used to evaluate the functional recovery after transplantation. The Olby scores of all groups were zero at the 0-week evaluation. At 2 week after the transplantation, the Olby scores in the groups with the UCB-MSC and UCBG were significantly higher than in the CN and CP groups. However, there were no significant differences between the UCB-MSC and UCBG groups, and between the CN and CP groups. These comparisons remained stable at 4 and 8 week after transplantation. There was significant improvement in the nerve conduction velocity based on the somatosensory evoked potentials. In addition, a distinct structural consistency of the nerve cell bodies was noted in the lesion of the spinal cord of the UCB-MSC and UCBG groups. These results suggest that transplantation of the UCB-MSCs resulted in recovery of nerve function in dogs with a spinal cord injury and may be considered as a therapeutic modality for spinal cord injury.

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Section: Discussionsupporting

confidence: 57%

Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs

et al. 2007

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“…Using a 15-point scoring system (Olby score), the dogs' gaits were independently scored from videotapes by 2 separate individuals who were blinded to the experimental conditions [37]. Mean scores at 1, 3, 5 and 9 weeks after SCI were calculated.…”

Section: Methodsmentioning

confidence: 99%

Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

et al. 2009

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In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression: C group (no ASCs treatment as control), V group (vehicle treatment with PBS), and ASC group (ASCs treatment). ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury. Pelvic limb function after transplantation was evaluated by Olby score. Magnetic resonance imaging, somatosensory evoked potential (SEP), histopathologic and immunohistichemical examinations were also performed. Olby scores in the ASC group increased from 2 weeks after transplantation and were significantly higher than C and V groups until 8 weeks (p < 0.05). However, there were no significant differences between the C and V groups. Nerve conduction velocity based on SEP was significantly improved in the ASC group compared to C and V groups (p < 0.05). Positive areas for Luxol fast blue staining were located at the injured site in the ASC group. Also, GFAP, Tuj-1 and NF160 were observed immunohistochemically in cells derived from implanted ASCs. These results suggested that improvement in neurological function by the transplantation of ASCs in dogs with spinal cord injury may be partially due to the neural differentiation of implanted stem cells.

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“…Behavioral recovery was scored according to the Olby scoring system [26], which is composed of 15 different criteria. The Olby scoring system is the modified scoring system for dogs based on pelvic limbs, and confirmed the reliability by several investigators [10,24,25]. The gaits of dogs were recorded using video camera, and two different investigators scored the gait.…”

Section: Behavioral Analysis Based On the Olby Scorementioning

confidence: 99%

A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury

Jung

Kang

et al. 2009

Journal of the Neurological Sciences

152

131

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Behavioural analysis of the efficacy of treatments for injuries to the spinal cord in animals

Cited by 27 publications

References 71 publications

Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs

Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs

Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury

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