Beiträge zur Papierchromatographie organischer Substanzen. VII. Die Dünnschichtchromatographie einiger Kohlehydratderivate und -Abbauprodukte

Synthetic Organic Products

“…PREY and co-workers [70] have ·separated glycerol (hRI 38) from ethylene glycol (hRI 45) on normal silica gel G layers, using butanolwater (90 + 10); the separation may be improved by impregnation with O.lN boric acid. They used dichromate-sulphuric acid (Rgt.…”

Glucose Fermentation Endproducts of Erwinia spp. and other Enterobacteria

“…The plates were developed in the Kontes sandwich chamber using 50 ml of solvent at a depth of c. 1 cm. The solvent mixture giving the best separation was absolute ethanol-concentrated ammonium hydroxide-distilled water in proportions of 80 + 4 + 16 (Prey, Berbalk & Kausz, 1962)) prepared immediately before use. Developing mixtures giving unsatisfactory or less satisfactory separation were : benzene-dioxaneglacial acetic acid (90 + 25 + 4) ; absolute ethanol; dioxane ; pyridine-diethyl ether (1 + 2) ; methanol-5.a NH,OH (80 + 20) ; diethyl ether; pyridine-petroleum ether (1 + 2) ; N-propanol-NH,OH (70 + 30) (Passera, Pedrotti & Ferrari, 1964) ; pyridineethyl acetate-water-glacial acetic acid (20 + 20 + 12 + 4) ; epiphase of ethyl acetateglacial acetic acid-water (40 + 20 + 40) ; methyl acetate-2.5% NH,OH V/V solution (95 + 5, Lynes, 1964), 70 + 30 and 80 + 20) ; Skellysolve B-acetone (70 + 30) ; absolute ethanol-NH,OH-water (80 + 4 + 16, 80 + 6 + 16, 90 + 4 + 10, 80 + 10 + 10 and 80 + 7 + 13).…”

Preparative Layer Chromatography

“…Of especial importance are impregnations with reagents forming complexes or addition compounds such as silver nitrate<i96,ii2,iio,26,i98,2oo,2io,227) (bonding silver ions with the TT-electrons of double bonds; thus, usuable for the separation of unsaturated lipids,^^^6) ^is and trans isomers of methyl linolates,^26) hydroxylated fatty acids,<2oo) waxes,^^i^) monoterpenes,<227) sesquiter penes, ^^^^ isomeric cyclododecanes),<2io)) boric acid, sodium borate as weU as sodium arsenate (207,214,215,114,197,198,199) (forming chelates; employed for the separation of vicinal dihydroxy isomers); sodium bisulfite(2) (addition compounds with carbonyl containing groups; applicable for the separation of carbohydrates), and trinitrobenzene (204) and picric acid^^^i) (forming complexes, employed for the separation of poly nuclear aromatics). Of especial importance are impregnations with reagents forming complexes or addition compounds such as silver nitrate<i96,ii2,iio,26,i98,2oo,2io,227) (bonding silver ions with the TT-electrons of double bonds; thus, usuable for the separation of unsaturated lipids,^^^6) ^is and trans isomers of methyl linolates,^26) hydroxylated fatty acids,<2oo) waxes,^^i^) monoterpenes,<227) sesquiter penes, ^^^^ isomeric cyclododecanes),<2io)) boric acid, sodium borate as weU as sodium arsenate (207,214,215,114,197,198,199) (forming chelates; employed for the separation of vicinal dihydroxy isomers); sodium bisulfite(2) (addition compounds with carbonyl containing groups; applicable for the separation of carbohydrates), and trinitrobenzene (204) and picric acid^^^i) (forming complexes, employed for the separation of poly nuclear aromatics).…”