During Australia’s unprecedented bushfires in 2019–2020, misinformation blaming arson surfaced on Twitter using . The extent to which bots and trolls were responsible for disseminating and amplifying this misinformation has received media scrutiny and academic research. Here, we study Twitter communities spreading this misinformation during the newsworthy event, and investigate the role of online communities using a natural experiment approach—before and after reporting of bots promoting the hashtag was broadcast by the mainstream media. Few bots were found, but the most bot-like accounts were social bots, which present as genuine humans, and trolling behaviour was evident. Further, we distilled meaningful quantitative differences between two polarised communities in the Twitter discussion, resulting in the following insights. First, Supporters of the arson narrative promoted misinformation by engaging others directly with replies and mentions using hashtags and links to external sources. In response, Opposers retweeted fact-based articles and official information. Second, Supporters were embedded throughout their interaction networks, but Opposers obtained high centrality more efficiently despite their peripheral positions. By the last phase, Opposers and unaffiliated accounts appeared to coordinate, potentially reaching a broader audience. Finally, the introduction of the bot report changed the discussion dynamic: Opposers only responded immediately, while Supporters countered strongly for days, but new unaffiliated accounts drawn into the discussion shifted the dominant narrative from arson misinformation to factual and official information. This foiled Supporters’ efforts, highlighting the value of exposing misinformation. We speculate that the communication strategies observed here could inform counter-strategies in other misinformation-related discussions.