Beneficial Effect of Bifidobacterium longum ATCC 15707 on Survival Rate of Clostridium difficile Infection in Mice

Yun, Bohyun; Song, Minyu; Oh, Sejong

doi:10.5851/kosfa.2017.37.3.368

Cited by 29 publications

(29 citation statements)

References 34 publications

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“…Thus, it is needed to select the optimal probiotic strain or combination of strains for prevention of CDI. Yun et al (2017) co-cultured one B. longum strain (ATCC 15707) with a C. difficile 027 strain in vitro and investigated the effect of ATCC 15707 on the mortality and intestinal damage through survival rates and histopathological analysis. They reported that ATCC 15707 treatment alleviated C. difficile induced damage revealed by histopathological analysis and improved survival rate ( Yun et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“… Yun et al (2017) co-cultured one B. longum strain (ATCC 15707) with a C. difficile 027 strain in vitro and investigated the effect of ATCC 15707 on the mortality and intestinal damage through survival rates and histopathological analysis. They reported that ATCC 15707 treatment alleviated C. difficile induced damage revealed by histopathological analysis and improved survival rate ( Yun et al, 2017 ). Differently, in our study, the anti-clostridial effects of B. longum JDM301 (JDM301), a widely used commercial probiotic strain in China, were evaluated comprehensively in vitro and in vivo , which was combined with comparative studies involving 40 strains of probiotic strains or candidates to reveal the anti-clostridial effects of Bifidobacterium .…”

Section: Discussionmentioning

confidence: 99%

“… Trejo et al (2013) reported that the B. bifidum strain CIDCA 5310 could antagonize the virulence of C. difficile in vivo . Yun et al (2017) showed beneficial effect of B. longum ATCC 15707 on survival rate of CDI in mice. However, the related studies remain scarce in vitro and in vivo , especially for commercially used strains ( Parkes et al, 2009 ; Evans and Johnson, 2015 ).…”

Section: Introductionmentioning

confidence: 89%

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Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile

Wei

Yang

et al. 2018

Front. Microbiol.

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Probiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains to inhibit C. difficile. This study examined the anti-clostridial effects of Bifidobacterium longum JDM301, a widely used commercial probiotic strain in China, in vitro and in vivo. In vitro evaluation revealed a significant reduction in C. difficile counts when JDM301 was co-cultured with C. difficile, which was correlated with the significant decrease in clostridial toxin titres (TcdA and TcdB). Furthermore, the cell-free culture supernatants (CFS) of JDM301 inhibited C. difficile growth and degraded TcdA and TcdB. Notably, the results showed that acid pH promoted the degradation of TcdA by CFS from JDM301. Furthermore, comparative studies among 10 B. longum strains were performed, which showed that the inhibitory effect of CFS from JDM301 was similar with the other 8 B. longum strains and higher than strain BLY1. However, when it was neutralized, the significant different was lost. When present together, it was suggested that the acid pH induced by probiotics not only played important roles in the growth inhibition against C. difficile resulting in the reduction of toxins titres, but also directly promoted the degradation of clostridial toxin. In vivo studies proved that JDM301 partially relieved damage to tissues caused by C. difficile and also decreased the number of C. difficile and toxin levels. In summary, our results demonstrated that the commercial strain, JDM301 could be considered a probiotic able to exert anti-toxin capability and most of the CFS from Bifidobacterium were able to inhibit the growth of C. difficile, depending on acid pH. These results highlighted a potential that JDM301 could be helpful in preventing CDI and that most of the bifidobacterial strains could (at least partially) exert protective effects by reducing toxin titres through growth inhibition against toxigenic C. difficile.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

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Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile

Wei

Yang

et al. 2018

Front. Microbiol.

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“…Mendes et al showed that preventive supplementation with B longum strain 51A attenuates exacerbated airway inflammation and hyperreactivity in ovariectomised mice. Another B longum strain, ATCC 157017, produces an organic acid capable of lowering pH and consequently inhibiting the growth of Clostridium difficile in mice . Furthermore, oral treatment with B longum strain 51A is effective against lung inflammation due to Klebsiella pneumoniae infection in mice …”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Bifidobacterium spp. in faeces of black howler monkeys (Alouatta pigra)

Hernández-Rodríguez

Vásquez-Aguilar

Serio-Silva

et al. 2018

J of Medical Primatology

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Background Bifidobacterium genus are considered to be beneficial bacteria for their hosts; however, knowledge about the specific species that are part of the gut microbiome of howler monkeys is scarce. Polymerase chain reaction (PCR) is a useful technique for the identification of non‐cultivable or difficult to grow bacterial species. With the goal of detecting species of the genus Bifidobacterium in black howler monkeys, we used PCR on DNA derived from faecal samples. Methods We collected and extracted DNA from 40 faecal samples. Using specific primers, we performed PCR and nested PCR to detect members of the Bifidobacterium genus and a subset of species: Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum and Bifidobacterium animalis subsp. animalis. Results 97.5% (39/40) of the samples were positive for Bifidobacterium spp. We found B longum in 100% of the analysed samples. Conclusions This is the first report of B longum in black howler monkey faeces.

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“…B. longum JCM 1217 (also known as ATCC 15707) is a typical probiotic strain that protects host from enteropathogenic infection ( Escherichia coli O157:H7) through production of acetate (Fukuda et al, 2011). The strain also reduced biofilm formation in pathogenic E. coli , reduced colitis in rats, and increased survival rate of Clostridium difficile infection in mice (Kim et al, 2012; Celiberto et al, 2017; Yun et al, 2017). If this strain was used as a host for foreign gene expression, its economy may improve as their synergistic effects.…”

Section: Introductionmentioning

confidence: 98%

Efficient Phytase Secretion and Phytate Degradation by Recombinant Bifidobacterium longum JCM 1217

et al. 2019

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Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous appA and is stable within Bifidobacterium longum JCM 1217. Cloning of three predicted promoters into pBPES proved that all of them drive appA expression in B. longum JCM 1217. Transformation of plasmids pBPES-tu and pBPES-groEL into B. longum JCM1217 resulted in much more phytase secretion suggests P tu and P groEL are strong promoters. Further in vitro and in vivo experiments suggested B. longum JCM 1217/pBPES-tu degrades phytate efficiently. In conclusion, the study screened two stronger promoters and constructed a recombinant live probiotic strain for effectively phytase secretion and phytate degradation in gut. The strategy used in the study provided a novel technique for improving the bioaccessibility of phytate and decreasing phosphorus excretion.

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Beneficial Effect of Bifidobacterium longum ATCC 15707 on Survival Rate of Clostridium difficile Infection in Mice

Cited by 29 publications

References 34 publications

Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile

Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile

Molecular detection of Bifidobacterium spp. in faeces of black howler monkeys (Alouatta pigra)

Efficient Phytase Secretion and Phytate Degradation by Recombinant Bifidobacterium longum JCM 1217

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