Beneficial effect of nerve growth factor‐7S on peripheral nerve regeneration through inside‐out vein grafts: An experimental study

Gravvanis, Andreas; Tsoutsos, Dimosthenis; Tagaris, G.; Papalois, Apostolos; Patralexis, Charalampos G.; Iconomou, Thomais; Panayotou, P.; Ioannovich, John

doi:10.1002/micr.20055

Cited by 61 publications

(39 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although initial reports focused on short gap repairs, 5,18,26 several substrates have been studied for their potential to heal large gap defects when supplemented with AGCs, including purified SCs. 1,6,7,9,11,12,16,17,27,28,34,37,38,[40][41][42]44 Berrocal et al demonstrated that adding autologous SCs suspended in serum to AGCs significantly enhanced the ability to bridge larger gap distances in sciatic nerve repair in rats. 3 Autologous SCs can be harvested from either a donor nerve or from the epicenter of the traumatized nerve ends in sharp injuries (propeller injury, gunshot wound, stab wound).…”

Section: Discussionmentioning

confidence: 99%

First human experience with autologous Schwann cells to supplement sciatic nerve repair: report of 2 cases with long-term follow-up

Gersey

Burks

Anderson

et al. 2017

FOC

View full text Add to dashboard Cite

OBJECTIVE Long-segment injuries to large peripheral nerves present a challenge to surgeons because insufficient donor tissue limits repair. Multiple supplemental approaches have been investigated, including the use of Schwann cells (SCs). The authors present the first 2 cases using autologous SCs to supplement a peripheral nerve graft repair in humans with long-term follow-up data. METHODS Two patients were enrolled in an FDA-approved trial to assess the safety of using expanded populations of autologous SCs to supplement the repair of long-segment injuries to the sciatic nerve. The mechanism of injury included a boat propeller and a gunshot wound. The SCs were obtained from both the sural nerve and damaged sciatic nerve stump. The SCs were expanded and purified in culture by using heregulin β1 and forskolin. Repair was performed with sural nerve grafts, SCs in suspension, and a Duragen graft to house the construct. Follow-up was 36 and 12 months for the patients in Cases 1 and 2, respectively. RESULTS The patient in Case 1 had a boat propeller injury with complete transection of both sciatic divisions at midthigh. The graft length was approximately 7.5 cm. In the postoperative period the patient regained motor function (Medical Research Council [MRC] Grade 5/5) in the tibial distribution, with partial function in peroneal distribution (MRC Grade 2/5 on dorsiflexion). Partial return of sensory function was also achieved, and neuropathic pain was completely resolved. The patient in Case 2 sustained a gunshot wound to the leg, with partial disruption of the tibial division of the sciatic nerve at the midthigh. The graft length was 5 cm. Postoperatively the patient regained complete motor function of the tibial nerve, with partial return of sensation. Long-term follow-up with both MRI and ultrasound demonstrated nerve graft continuity and the absence of tumor formation at the repair site. CONCLUSIONS Presented here are the first 2 cases in which autologous SCs were used to supplement human peripheral nerve repair in long-segment injury. Both patients had significant improvement in both motor and sensory function with correlative imaging. This study demonstrates preliminary safety and efficacy of SC transplantation for peripheral nerve repair.

show abstract

Section: Discussionmentioning

confidence: 99%

First human experience with autologous Schwann cells to supplement sciatic nerve repair: report of 2 cases with long-term follow-up

Gersey

Burks

Anderson

et al. 2017

FOC

View full text Add to dashboard Cite

show abstract

“…One of the objective assessments of nerve regeneration developing after peripheral nerve injury is the evaluation of the numerical distribution and spatial orientation of myelinated and unmyelinated nerve fibers [19] . According to the EM assessment, the greatest similarity to controls was found in the first month in the high-dose MP+-VA and normal-dose MP treatment groups.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Methylprednisolone and Vitamin A on the Healing of Traumatic Peripheral Nerve Paralysis

Sevük¹,

Vayısoğlu²,

Korlu³

et al. 2015

Int Adv Otol

View full text Add to dashboard Cite

OBJECTIVE:This study planned to evaluate the effects of vitamin A (VA) and steroid treatment on nerve healing. Study design: we investigated electrophysiological, electron microscopic (EM), immunohistochemical, and biochemical findings in rat sciatic nerve model. MATERIALS and METHODS:In total, 112 female rats were divided into 16 groups, each consisting of 7 animals. Subjects were evaluated according to compound muscle action potential (CMAP) findings, ultrastructural staging of myelinated axons with EM, nitric oxide (NO) and malondialdehyde (MDA) findings in serum and tissue, and immunohistochemical findings in the first week and first month. RESULTS:NO and MDA levels in serum and tissue were found to be statistically lower after high-dose methylprednisolone (MP), normal-dose MP, high-dose MP+VA, normal-dose, and MP+VA treatment modalities compared to controls (p<0.05). The most similar EM findings compared to control animals were seen in the normal-dose MP and high-dose MP+VA treatment groups. The best amplitude values were seen in the high-dose MP and continuous normal-dose MP therapy groups in the first month. In the normal-dose MP and high-dose MP+VA treatment groups, intense staining was found compared to the control and sham groups according to the sciatic nerve immunohistochemical examination. CONCLUSION:This study revealed noticeable VA effects on the inflammatory process during the healing of traumatic nerve paralysis by electron microscopy and immunohistochemistry. However, similar findings were not established by electrophysiology. Therefore, more experimental studies are needed to further investigate the efficacy and the mechanism of action of VA on steroid administration and the inflammatory process.

show abstract

“…They also play a role in clearing debris and creating an appropriate milieu for nerve regrowth. Due to their role in nerve regeneration, the behavior of SCs on a biomaterial used for nerve conduit fabrication is clearly a key issue (Guenard, Kleitman, Morrissey, Bunge, & Aebischer, 1992;Heath & Rutkowski, 1998) and it has been shown that SCs can be aligned by culturing them on biomaterial surface groves which provide micrometric dimensions .…”

Section: Chitosan Conduits Combined With Cells For Pns Repairmentioning

confidence: 99%

“…Nerve regeneration has been found to be enhanced by utilizing guidance channels filled with neurotrophic factors, such as GDNF, ciliary nerotrophic factor (CNTF), FGF-2, and NT-3 (Grothe, Haastert, & Jungnickel, 2006;Madduri, Feldman, Tervoort, Papaloizos, & Gander, 2010;Madduri, Papaloizos, & Gander, 2010;Oh et al, 2008;Pfister et al, 2008;Yang et al, 2007). Various types of nerve guides have been developed by blending chitosan with different growth factors (Table 1.8).…”

Section: Chitosan Conduits Combined With Neurotrophic Factors or Neurmentioning

confidence: 99%

“…Also neurosteroids, such as progesterone (PROG) and pregnenolone (PREG), have been used for potentiating conduit nerve repair as these hormones are synthesized by SCs (Baulieu & Schumacher, 2000) and induce myelination binding on intracellular receptors which activate the synthesis of myelin protein P0 and PMP22 (Desarnaud et al, 1998;Jung-Testas, Schumacher, Robel, & Baulieu, 1996). Furthermore, neurite outgrowth may be stimulated by a PROG metabolite (5-a-tetrahydroprogesterone) through GABA(A) receptors (Guennoun et al, 2001;Koenig, Gong, & Pelissier, 2000). Chitosan conduits have been used to deliver PROG into a 10-mm rabbit facial nerve gap model.…”

Section: Chitosan Conduits Combined With Neurotrophic Factors or Neurmentioning

confidence: 99%

See 1 more Smart Citation

The Use of Chitosan-Based Scaffolds to Enhance Regeneration in the Nervous System

Gnavi

Barwig²,

Freier³

et al. 2013

International Review of Neurobiology

View full text Add to dashboard Cite

Various biomaterials have been proposed to build up scaffolds for promoting neural repair. Among them, chitosan, a derivative of chitin, has been raising more and more interestamongbasicandclinicalscientists.Anumberofstudieswithneuronalandglial cellcultures haveshownthat thisbiomaterial has biomimetic properties,which make it a good candidate for developing innovative devices for neural repair. Yet, in vivo experimental studies have shown that chitosan can be successfully used to create scaffolds that promote regeneration both in the central and in the peripheral nervous system. In this review, the relevant literature on the use of chitosan in the nervous tissue, either alone or in combination with other components, is overviewed. Altogether, the promising in vitro and in vivo experimental results make it possible to foresee that time for clinicaltrialswithchitosan-basednerveregenerationpromotingdevicesisapproaching quickly.

show abstract

Beneficial effect of nerve growth factor‐7S on peripheral nerve regeneration through inside‐out vein grafts: An experimental study

Cited by 61 publications

References 38 publications

First human experience with autologous Schwann cells to supplement sciatic nerve repair: report of 2 cases with long-term follow-up

First human experience with autologous Schwann cells to supplement sciatic nerve repair: report of 2 cases with long-term follow-up

The Effects of Methylprednisolone and Vitamin A on the Healing of Traumatic Peripheral Nerve Paralysis

The Use of Chitosan-Based Scaffolds to Enhance Regeneration in the Nervous System

Contact Info

Product

Resources

About