Beneficial effects of dl-α-lipoic acid on cyclophosphamide-induced oxidative stress in mitochondrial fractions of rat testis

Elangovan, Selvakumar; Prahalathan, Chidambaram; Mythili, Yenjerla; Varalakshmi, P.

doi:10.1016/j.cbi.2005.01.009

Cited by 55 publications

(40 citation statements)

References 35 publications

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“…Meanwhile, antioxidant ability previously destroyed by CP was signifi cantly restored in mice administered with SIP. Increasing evidence to support the idea that the toxicity of CP can be weakened by some natural materials (5,6,8,9) including SIP, further indicates that these compounds are potential cytoprotectors that could be applied in clinical therapy for cancer.…”

Section: Discussionmentioning

confidence: 99%

“…LDH, correlated to the seminiferous epithelium, is in mitochondrial membranes of mature or maturing testicular spermatogenic cells and plays critical roles in the proton transfer process across mitochondrial membranes. Peroxidation of mitochondrial membranes induced by CP negatively affects activity of LDH, spermatogenesis, and maturation of testis (8). Gamma-GT is connected with maturation and proliferation of Sertoli cells, CP-induced increases in its activity means damaged Sertoli cells and disturbed spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Le¹,

Luo²,

Gu³

et al. 2015

BLL

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Cyclophosphamide (CP) is a commonly used antitumour and immunosuppressive drug, but it is inevitable that the chemotherapeutic agent may cause long-term or permanent reproductive damage on young male patients through inducing oxidative stress in the testes. Squid ink polysaccharides (SIP), a newly found marine glycosaminoglycon have been proved to have antioxidant capabilities and chemotherapy-protective activities on model animals in our recent investigations. This study was conducted to assess whether or not SIP could protect male mice against gonadotoxicity during CP exposure. Sexually mature male Kunming mice were allocated to one of four groups. CP was abdominally administered at dose of 15 mg/kg body weight to two groups of mice for ten weeks, once a week, one group of mice received SIP at dose of 80 mg/kg body weight by gavage for ten weeks, once a day. The other two groups comprised a vehicle treated group and an SIP treated group. Toxicity of CP and protective activity of SIP on the testes were assessed by: sperm parameters, organ index, testicular antioxidant ability, activities of marker enzymes, sex hormone content, and histopathological features. Data showed CP-induced, serious negative changes on murine sperm parameters, organ index, testicular antioxidant ability, activities of marker enzymes, sexual hormone contents, and histopathological features which were all signifi cantly impaired by SIP. This study found that SIP were demonstrated to offer protective effects against CP-induced toxicity on testes in mice (Tab. 2, Fig. 3, Ref. 29). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Le¹,

Luo²,

Gu³

et al. 2015

BLL

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“…It was also observed decreased testis, seminal vesicles and epididymal weights, damage and decreased number of spermatogonial cells in the seminiferous tubules, low levels of plasma testosterone and infertility (ELANGOVAN et al, 2006;REZVANFAR et al, 2008). Increased levels of lipid peroxidation and malondialdehyde and reduced levels of antioxidant enzymatic systems (gluthatione reductase, gluthatione peroxidase, catalase, superoxide dismutase) were detected in CP damaged testis (ILBEY et al, 2009;MANDA;BHATIA, 2003;SELVAKUMAR et al, 2004;REZVANFAR et al, 2008;MOTAWI et al, 2010). The main findings on testicular morphology in CP treated rats were the impairment of germ cell line maturation, highlighted by decreased Johnsen's testicular score, atrophy and degeneration of seminiferous tubules, vacuolization in Sertoli cells, interstitial edema and degeneration of Leydig cells (CERIBASI et al, 2010;MOTAWI et al, 2010;REZVANFAR et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Biological Sciences Maringá, v. 35, n. 1, p. 105-113, Jan.-Mar., 2013 SELVAKUMAR et al, 2006;TURK et al, 2010). CP toxic effects on testis were mainly attributed to oxidative stress on seminiferous tubules and Sertoli cells, impairing spermatogenis and androgenesis, and inducing germinal cells apoptosis (ILBEY et al, 2009;MOTAWI et al, 2010;REZVANFAR et al, 2008;TURK et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…The use of antioxidant agents seemed partially to protect male reproductive organs from CPb toxic effects in animals models (CARMELY et al, 2009;SELVAKUMAR et al, 2004SELVAKUMAR et al, , 2005CERIBASI et al, 2010;ILBEY et al, 2009;MOTAWI et al, 2010;TURK et al, 2010). These studies suggest that increasing antioxidant defenses may have some protective effects over drug cytotoxicity in male reproductive tract.…”

Section: Introductionmentioning

confidence: 99%

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Exercise protects rat testis from cyclophosphamide-induced damage

Ramos

Goessler

Ruíz

et al. 2013

Acta Sci. Biol. Sci.

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ABSTRACT.To investigate the effect of chronic moderate exercise on male reproductive tract of Wistar rats submitted to a single dose of cyclophosphamide (CP). Animals were submitted to swimming exercise during 21 days or maintained at sedentary state. Trained (TCP) and sedentary (SCP) groups received a single dose of CP (200 mg kg -1 , i.p.). Trained (TCo) and sedentary (SCo) control animals received sterile PBS. Animals were killed after one week and testis, epidydimis and seminal vesicle contend were weighted. Testis were embebbed in parafim and stained with hemotaxilin and eosin. Fifty seminiferous tubules of each animal were analyzed by Johnsen score. Mean Sertoli cells counts per tubule and Leydig cells counts per area were evaluated. CP treatment impairs body weight gain in trained and sedentary animals. Liver and seminal vesicle contend were reduced only in SCo group. SCP animals presented decreased Johnsen scores, indicating a slight toxicity over germinative cells, whereas trained (TCo and TCP) animals presented increased Johnsen scores. Training increased Sertoli cell counts and prevented their loss in TCP group. Leydig cells counts were increased in trained animals, but decreased in CP treated ones (TCP). We conclude that exercise have some protective effect on male reproductive tract submitted to a single dose of CP. , ip). Os animais treinados (TCO) e sedentários (SCO) receberam PBS estéril. Os animais foram sacrificados após uma semana e os testículos, epidídimo e conteúdo da vesícula seminal foram pesados. As amostras foram embebidas em parafina e coradas com hematoxilina e eosina. Cinquenta túbulos seminíferos de cada animal foram analisados pelo escore Johnsen. A contagem média das células de Sertoli por túbulo e contagem das células de Leydig por área foram avaliadas. O tratamento CP prejudicou o ganho de peso corporal em animais treinados e sedentários. O fígado e o conteúdo da vesícula seminal foram reduzidos apenas no grupo SCO. Os animais SCP apresentaram menores escores de Johnsen, indicando uma toxicidade moderada sobre as células germinativas, enquanto os animais treinados (TCO e TCP) apresentaram escores de Johnsen mais altos. O treinamento aumentou a contagem de células de Sertoli e impediu a sua perda no grupo TCP. A contagem das células de Leydig foram aumentadas em animais treinados, mas reduzidas nos animais tratados com CP (TCP). Concluiu-se que o exercício tem algum efeito protetor sobre trato reprodutivo masculino de animais submetidos a uma dose única de CP.Palavras-chave: ciclofosfamida, exercício, trato reprodutiva.

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Catechin as an antioxidant in liver mitochondrial toxicity: Inhibition of tamoxifen‐Induced protein oxidation and lipid peroxidation

Tabassum

Parvez

Rehman

et al. 2007

J Biochem & Molecular Tox

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Tamoxifen (TAM) is a nonsteroidal triphenylethylene antiestrogenic drug widely used in the treatment and prevention of breast cancer. TAM brings about a collapse of the mitochondrial membrane potential. It acts both as an uncoupling agent and as a powerful inhibitor of mitochondrial electron transport chain. The effect of catechin pretreatment on the mitochondrial toxicity of TAM was studied in liver mitochondria of Swiss albino mice. TAM treatment caused a significant increase in the mitochondrial lipid peroxidation (LPO) and the protein carbonyls (PCs). It also caused a significant increase in superoxide radical production. Pretreatment of mice with catechin (40 mg/kg) showed significant protection as demonstrated by marked attenuation of increased oxidative stress parameters such LPO, PCs, and superoxide production. It also restored the decreased nonenzymatic and enzymatic antioxidants of mitochondria. The inhibitory effect of catechin on TAM-:induced oxidative damage suggests that it may have potential benefits in prevention of human diseases where reactive oxygen species have some role as causative agents.

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Beneficial effects of dl-α-lipoic acid on cyclophosphamide-induced oxidative stress in mitochondrial fractions of rat testis

Cited by 55 publications

References 35 publications

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Interventional effects of squid ink polysaccharides on cyclophosphamide-associated testicular damage in mice

Exercise protects rat testis from cyclophosphamide-induced damage

Catechin as an antioxidant in liver mitochondrial toxicity: Inhibition of tamoxifen‐Induced protein oxidation and lipid peroxidation

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