Beneficial effects of eugenol supplementation on gut microbiota and hepatic steatosis in high-fat-fed mice

Rodrigues, Marina; Bertoncini-Silva, Caroline; Joaquim, Anderson Gregorio; Machado, Cássia Dias; Ramalho, Leandra Naíra Zambelli; Carlos, Daniela; Fassini, Priscila Giácomo; Suen, Vívian Marques Miguel

doi:10.1039/d1fo03619j

Cited by 17 publications

(6 citation statements)

References 53 publications

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“…[ 23 ] The dose of EU used for mice in this study (200 mg kg −1 ) was lower than the dose of no‐observed‐adverse‐effect‐level, and the doses used in other studies. [ 24 ]…”

Section: Methodsmentioning

confidence: 99%

Eugenol, A Major Component of Clove Oil, Attenuates Adiposity, and Modulates Gut Microbiota in High‐Fat Diet‐Fed Mice

Zhao

Wang

et al. 2022

Molecular Nutrition Food Res

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Scope Eugenol (EU), the major aromatic compound derived from clove oil, is being focused recently due to its potential in preventing several chronic conditions. Herein, this study aims to evaluate the potential of EU in obesity prevention and to delineate the mechanisms involved. Methods and results Five‐week‐old male C57BL/6J mice are fed with high‐fat diet (HFD) or HFD supplemented with EU (0.2%, w/w) for 13 weeks. EU significantly reduces obesity‐related indexes including final body weight, body weight gain, adipocyte size, visceral fat‐pad weight, and fasting blood glucose. EU prevents HFD‐induced gut dysbiosis, as indicated by the increase of Firmicutes and decrease of Desulfobacterota at phylum level, and the increase of Dubosiella, Blautia, unclassified_f_Oscillospiraceae, and unclassified_f_Ruminococcaceae, and the decrease of Alistipes, Alloprevotella, and Bilophila at genus level. Notably, the obesity‐related indexes are positively correlated with the relative abundances of Bacteroides, unclassified_f_Lachnospiraceae, Colidextribacter, and Bilophila, and negatively correlated with the relative abundances of norank_f_Muribaculaceae and Lachnospiraceae_NK4A136_group. Moreover, the preventive effects of EU on obesity are accompanied by the transcriptomic reprogramming of white adipose tissue. Conclusion These findings demonstrate that EU prevents the HFD‐induced adiposity and modulates gut dysbiosis, and highlight the potential of EU in obesity intervention as a functional dietary supplement.

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“…[ 23 ] The dose of EU used for mice in this study (200 mg kg −1 ) was lower than the dose of no‐observed‐adverse‐effect‐level, and the doses used in other studies. [ 24 ]…”

Section: Methodsmentioning

confidence: 99%

Eugenol, A Major Component of Clove Oil, Attenuates Adiposity, and Modulates Gut Microbiota in High‐Fat Diet‐Fed Mice

Zhao

Wang

et al. 2022

Molecular Nutrition Food Res

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“…Research has shown that the makeup of gut bacteria plays a role in the development of obesity [ 1 ]. Changes in diet, whether temporary or permanent, impact the composition of intestinal bacteria [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…Obesity is a glucose and lipid metabolic disease involving the abnormal buildup of fat in the body, leading to various health problems, including hypertension, hyperlipidemia, cardiovascular disease, and type 2 diabetes [ 1 , 2 ]. There are now almost 2 billion overweight adults in the world, and this trend continues to worsen, making obesity a global epidemic [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Zhu,

Hou

2024

Foods

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Lactarius hatsudake (LH), a great wild endemic fungus, contains rich nutritional components with medicinal properties. The effects of LH on body weight, liver weight, liver injury, blood lipids, and gut microbiota in C57BL/6 mice fed a high-fat diet (HFD) for 8 weeks was examined in this research. Though there was no clear impact on weight loss, the findings indicate that LH treatment effectively decreased liver damage caused by HFD, as well as lowered serum total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels. Additionally, it positively influenced gut microbiota to resemble that of mice on a normal diet. In HFD-fed mice, LH markedly boosted the levels of Parabacteroides, unclassified Muribaculaceae, Oscillibacter, and unclassified Oscillospiraceae, while reducing the abundance of Lachnospiraceae NK4A136 group and Erysipelatoclostridium, as well as the ratio of Firmicutes to Bacteroidetes. Further analysis of correlation indicate a possible connection between obesity and gut microbiota. Obesity-related indices show a positive association with unclassified Eubacterium coprostanoligenes group, Blautia, and Erysipelatoclostridium, while displaying a negative correlation with unclassified Muribaculaceae, unclassified Clostridia vadinBB60 group, Helicobacter, Oscillibacter, unclassified Ruminococcaceae, Parabacteroides, and unclassified Oscillospiraceae. The results suggest that LH can help combat obesity and may have the potential to be utilized as a functional food.

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“…They also prevent metabolic alterations arising from obesity and hepatic steatosis, by reducing the accumulation of lipids, an effect mediated in part by the transient vanilloid receptor 1 (TRPV1) expressed in the functional regulation of the liver and adipose tissue. The activation of signaling of the TRPV1 promotes the accumulation of fat, while the inhibition of this signaling protects against this buildup [50].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

de Oliveira Ramos Melo,

da Costa Marques,

Neto

et al. 2024

FNS

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Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of phenolic compounds, such as flavonoids, terpenoids and eugenol. In turn, the most common uses of gum arabic are in the form of powder for addition to soft drink syrups, cuisine and baked goods, specifically to stabilize the texture of products, increase the viscosity of liquids and promote the leavening of baked products (e.g., cakes). Both eugenol, extracted from cloves, and gum arabic, extracted from the hardened sap of two species of the Acacia tree, are dietary constituents routinely consumed virtually throughout the world. Both of them are also widely used medicinally to inhibit oxidative stress and genotoxicity. The prevention arm of the study included groups: Ia,

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Beneficial effects of eugenol supplementation on gut microbiota and hepatic steatosis in high-fat-fed mice

Abstract: Due to the increase in the prevalence of obesity, new therapies have emerged and Eugenol has been shown to be beneficial in metabolic changes and gut microbiota. This study aimed...

Cited by 17 publications

References 53 publications

Eugenol, A Major Component of Clove Oil, Attenuates Adiposity, and Modulates Gut Microbiota in High‐Fat Diet‐Fed Mice

Eugenol, A Major Component of Clove Oil, Attenuates Adiposity, and Modulates Gut Microbiota in High‐Fat Diet‐Fed Mice

Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

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