Beneficial effects of hydrogen gas inhalation on a murine model of allergic rhinitis

Abstract

Allergic rhinitis (AR) is a common chronic inflammatory condition. It has been previously indicated that oxidative stress may contribute to allergic inflammation, including AR. Although molecular hydrogen (H2), an antioxidative agent, has been effective in treatment of numerous oxidative stress-associated diseases, the effect of inhalation of a high concentration of H2 on AR remains unknown. In the current study, female BALB/c mice were sensitized with ovalbumin (OVA) followed by intranasal OVA challenge to establish an animal model of AR. Mice were subjected to exposure to H2 and the inert gas helium at different frequencies and durations. The frequencies of sneezing/scratching and the body weights of mice were recorded. Histological analysis and multiplex cytokine assays were performed to evaluate the effects of H2 on AR. Challenge with OVA induced significant nasal mucosa inflammation. H2 inhalation reduced the infiltration of inflammatory cells into mucosa and lowered the levels of interleukin (IL)-5, IL-13 and monocyte chemoattractant protein-1 in serum. H2 inhalation slightly increased the level of interferon-γ, however the difference was not statistically significant. Treatment with H2 limited the weight increase in healthy mice and reversed the weight loss in mice with AR. Furthermore, H2 inhalation induced a therapeutic effect on AR in a dose-dependent manner. The current results demonstrate that H2 may demonstrate a therapeutic value for allergic diseases.

Low-Flow Nasal Cannula Hydrogen Therapy

“…The health benefits include improving mood, reducing anxiety, and counteracting an overactive sympathetic nervous system [ 1 ]. In terms of disease prevention and treatment, H 2 has been used to treat many diseases, including lifestyle diseases [ 2 ]; immune diseases, such as atopic dermatitis [ 2 ], hay fever [ 3 ], and chronic rheumatoid arthritis [ 4 ]; respiratory diseases, such as asthma [ 5 ], chronic obstructive pulmonary disease [ 6 ], and pneumonia caused by the coronavirus disease 2019 (COVID-19) [ 7 ]; neurological diseases, such as depression [ 8 ], dementia [ 9 ], stroke [ 10 ], post-cardiac arrest syndrome [ 11 , 12 ], subarachnoid hemorrhage [ 13 ], and traumatic injury from blast shock waves [ 14 ]; myocardial infarction [ 15 - 17 ]; chronic kidney disease [ 18 ]; sepsis [ 19 ] and hemorrhagic shock [ 20 , 21 ]; and cancer [ 22 ].…”

Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation

“…We previously reported the use of histological and cellular factor analyses to evaluate the effect of hydrogen on ovalbumin-induced sinus mucosal inflammatory reaction (allergic rhinitis, AR) in mice. The results showed that hydrogen inhalation significantly alleviated the symptoms of sneezing and pruritus, reduced the infiltration of inflammatory cells into the mucosa, and decreased the levels of IL-5, IL-13, and MCP-1 in serum, indicating the value of hydrogen in treating allergic diseases (Fang et al, 2018).…”

A narrative review of hydrogen-oxygen mixture for medical purpose and the inhaler thereof

“…With the support of hydrogen inhaler AMS-H-01/AMS-H-03 provided by Asclepius Meditec Co., Ltd., tens of animal studies or pilot clinical trials based on inhaled gas which combines H 2 (concentration of ~66%) with O 2 (concentration of ~34%) were accomplished and published. 14 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 Additionally, recent formal clinic trials based on the same inhaler AMS-H-01/AMS-H-03 also revealed positive results in respiratory diseases, such as allergic rhinitis, asthma, chronic obstructive pulmonary disease, etc. Especially, it is recommended to use the mixed gas of 66.6% H 2 /33.3% O 2 for treating coronavirus disease-19 (COVID-19) patients as described in the Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia, 7 Edition released by National Health Commission of China, 55 and such guideline is also republished in English by the World Health Organization China Office.…”