Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Ma, Zhihong; Chu, Li; Liu, Hongying; Wang, Weijie; Li, Jieru; Yao, Wenzao; Yi, Jianfeng; Gao, Yue

doi:10.1038/srep44819

Cited by 95 publications

(61 citation statements)

References 30 publications

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“…Histopathology in this study showed that HFD displayed characteristic features of nonalcoholic steatohepatitis (NASH). These results were in agreement with results of Ma et al [27] Oral administrations of C. olitorius extract (300 or 400 mg/mg/kg) or orlistat reduced hepatic fat accumulation, alleviated swelling of hepatocytes and prevented the formation of steatosis. The C. olitorius extract was significantly more effective in comparison with orlistat and displayed at the nearly normal appearance compared to the ND control group.…”

Section: Discussionsupporting

confidence: 93%

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Gomaa

Elsers

Al-Zokeim

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives To determine the efficacy of Corchorus olitorius (C. olitorius) leaf extract in the prevention of metabolic syndrome induced in rats by high‐fat diet (HFD) and compare it with that of orlistat. Methods Phytochemical analysis was performed. Effect of orlistat and C. olitorius extract on lipase activity and acute food intake were investigated. Body weight, biochemical parameters and histopathological examination were demonstrated. Key findings Corchorus olitorius extract inhibited the pancreatic lipase activity, but orlistat was more potent. Cumulative food intake has not changed by the tested agents. In obese rats, C. olitorius or orlistat significantly decreased weight gain and visceral white adipose tissue. They exhibited a significant reduction in serum glucose, total cholesterol, triglycerides, low density lipoprotein cholesterol, free fatty acids, IL‐1β, tumour necrosis factor‐α (TNF‐α), insulin and leptin levels of obese rat groups while high density lipoprotein cholesterol and adiponectin levels were significantly increased by them. Histopathological examination of the liver revealed that C. olitorius was more effective than orlistat in the alleviating of steatosis and adipocyte hypertrophy shown in obese control rats. Conclusions Corchorus olitorius is effective as orlistat in preventing obesity, hyperlipidaemia, steatosis and insulin resistance. These actions may be mediated by inhibiting of lipase activity, TNF‐α, IL‐1β and leptin resistance along with increasing of adiponectin.

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Section: Discussionsupporting

confidence: 93%

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Gomaa

Elsers

Al-Zokeim

et al. 2019

Journal of Pharmacy and Pharmacology

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“…From each rat, a skeletal muscle sample (100-150 g) was obtained and homogenized in phosphate buffer (pH 7.0) at 4 ∘ C with a homogenizer (UP100H, Hielscher Ultrasonics, Berlin, Germany). The homogenized tissue was centrifuged at 12000 rpm at 4 ∘ C for 15 min [19], and the supernatant was collected for gene expression analysis. Additionally, a blood sample was collected from the abdominal aorta for measurement of the blood insulin and glucose levels.…”

Section: Sample Collection and Measurementsmentioning

confidence: 99%

Effects of Exercises on IL-6 and GLUT4 Expression in Diabetic Rats

Dehghan

Farzanegi

Abbaszadeh

2019

RMM

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Background: Diabetes mellitus is one of the most common metabolic disorders worldwide. Empirical evidence has shown exercise to be of value in ameliorating symptoms of diabetes, but the underlying molecular mechanisms involved have not been well-studied. To evaluate the effects of continuous and interval exercises on blood glucose and insulin levels and on IL-6 and GLUT4 expression in diabetic rats. Materials and Methods: Old Wistar rats with streptozotocin-induced diabetes were randomly divided into three groups; namely, the patient (non-exercise control) group, the interval exercise group, and the continuous exercise group. The blood insulin level was measured using a specific ELISA kit, whereas the blood glucose level was determined with an AutoAnalyzer apparatus. Expression of the IL-6 and GLUT4 genes in skeletal muscle was determined using real-time PCR. All data were analyzed using SPSS software. Results: The expression of IL-6 and GLUT4 was significantly decreased in skeletal muscle of the diabetic rats (p < 0.001). Continuous (9.97-fold; p = 0.000) and interval (7.11-fold; p = 0.000) exercises significantly increased the expression of IL-6 relative to that in the animals of the patient group. Furthermore, continuous (9.36-fold; p = 0.000) and interval (7.65-fold; p = 0.001) exercises also significantly increased the expression of GLUT4 relative to that of the patient group. Both types of exercise training were associated with a significant decrease in the blood glucose and insulin levels compared with the levels in the patient group (p < 0.0001). Conclusions: Diabetes is significantly associated with IL-6 and GLUT4 downregulation, but this can be reversed through continuous and interval exercises, which also help to lower the blood glucose and insulin levels significantly.

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“…A study demonstrated that Rg1 and Rb1 were involved improving lipid levels and ameliorate in ammation in NAFLD mice [28]. Paeoni orin has obvious effects on suppressing lipid ectopic deposition, enhancing insulin sensitivities and decreasing oxidation in NAFLD rats [29,30]. Astragaloside has obvious anti-lipid accumulation effects through regulating AMPK signal in NAFLD cell models [31,32].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: The mechanism of jian-Gan-Xiao-Zhi decoction in IR adipocytes and its component analysis

Fang

Wen

Xie

et al. 2020

Preprint

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Background: Jian-Gan-Xiao-Zhi decoction (JGXZ) is a formula of traditional Chinese medicine to treat patients with NAFLD. Thus, the study aimed to analyze the mechanism of drug serum of JGXZ in adipocytes and detect the main components of JGXZ in rat serum. Methods: The 3T3-L1 preadipocytes were used to establish IR adipocytes model. The lipid accumulation in adipocytes was detected by oil red O staining. After JGXZ treatment, glucose consumption, TC and TG were analyzed by corresponding kits. ROS levels were measured by flow cytometry. In addition, Western Blot was used to assess LKB1/AMPK and JNK/IRS/PI3k/AKT expressions. Except for that, the main components of JGXZ in rat serum samples were detected by LC-MS/MS method. A Phenomenex Luna C18 was used to perform chromatographic separation and detect the concentration of main components in JGXZ. The ion source was ESI. The mobile phase contained methanol and 0.1% formic acid solution. Results: JGXZ significantly decreased glucose levels and adipogenesis, accompanied by decreased IR (p<0.01). Besides, JGXZ markedly affected ROS, LKB1/AMPK and JNK/IRS/PI3k/AKT levels(p<0.01). The R1, Rg1, paeoniflorin, Rb1, astragaloside and tanshinone could be significantly quantified. Conclusions: Thus, JGXZ decreased glucose and lipid synthesis possibly via ROS/AMPK/JNK pathway. R1, Rg1, paeoniflorin, Rb1, astragaloside and tanshinone in JGXZ could play main roles in treating NAFLD, which could offer assistance for us to study the mechanism of JGXZ on treating NAFLD.

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Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Cited by 95 publications

References 30 publications

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines

Effects of Exercises on IL-6 and GLUT4 Expression in Diabetic Rats

WITHDRAWN: The mechanism of jian-Gan-Xiao-Zhi decoction in IR adipocytes and its component analysis

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