Beneficial Effects of Prebiotic<i>Saccharomyces cerevisiae</i>Mannan on Allergic Asthma Mouse Models

Michael, Christie F.; Overbeck, T.; Robinson, W. Scout; Rohman, Erin L.; Lehman, Jeffrey M.; Patel, Jennifer K.; Eiseman, Brandi; LeMessurier, Kim S.; Samarasinghe, Amali E.; Gaber, M. Waleed

doi:10.1155/2017/3432701

Cited by 14 publications

(12 citation statements)

References 35 publications

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“…Mannosyl-rich lysosomal hydrolases, such as β-HEX, are protease-resistant (and thus stable) in plasma until cleared by their receptors. A number of cell types such as mast cells, macrophages and T cells can secrete these enzymes [ 41 , 42 ]. We previously reported that β-HEX and its receptor (MRC2, calcium-dependent mannose receptor 2) have a putative role in airway smooth muscle remodeling [ 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Questioning Cause and Effect: Children with Severe Asthma Exhibit High Levels of Inflammatory Biomarkers Including Beta-Hexosaminidase, but Low Levels of Vitamin A and Immunoglobulins

et al. 2020

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Asthma affects over 8% of the pediatric population in the United States, and Memphis, Tennessee has been labeled an asthma capital. Plasma samples were analyzed for biomarker profiles from 95 children with severe asthma and 47 age-matched, hospitalized nonasthmatic controls at Le Bonheur Children’s Hospital in Memphis, where over 4000 asthmatics are cared for annually. Asthmatics exhibited significantly higher levels of periostin, surfactant protein D, receptor for advanced glycation end products and β-hexosaminidase compared to controls. Children with severe asthma had lower levels of IgG1, IgG2 and IgA, and higher levels of IgE compared to controls, and approximately half of asthmatics exhibited IgG1 levels that were below age-specific norms. Vitamin A levels, measured by the surrogate retinol-binding protein, were insufficient or deficient in most asthmatic children, and correlated positively with IgG1. Which came first, asthma status or low levels of vitamin A and immunoglobulins? It is likely that inflammatory disease and immunosuppressive drugs contributed to a reduction in vitamin A and immunoglobulin levels. However, a nonmutually exclusive hypothesis is that low dietary vitamin A caused reductions in immune function and rendered children vulnerable to respiratory disease and consequent asthma pathogenesis. Continued attention to nutrition in combination with the biomarker profile is recommended to prevent and treat asthma in vulnerable children.

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Section: Discussionmentioning

confidence: 99%

Questioning Cause and Effect: Children with Severe Asthma Exhibit High Levels of Inflammatory Biomarkers Including Beta-Hexosaminidase, but Low Levels of Vitamin A and Immunoglobulins

et al. 2020

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“…While fungal wall components can result in allergic inflammation, herein, we showed that MN derived from S. cerevisiae did not limit features of allergic asthma induced by A. fumigatus . Since MN can alter the pathogenesis of ovalbumin-induced allergic airways disease [ 16 ], it is possible that this yeast component is not effective when the triggering allergen is also fungal. Further understanding on mannose receptor expression and regulation may be beneficial to explore MN as a potential therapeutic to limit allergic inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…The human mannose receptor 2 (huMRC2) cloned from bronchial smooth muscle cells was overexpressed in a mouse using smooth muscle specific SM22 α gene promotor. Mice overexpressing huMRC2 were backcrossed onto BALB/c mice and N10 generation mice were used for experiments [ 16 ]. Mice were housed in microisolator cages with α -dri bedding with free access to chow in a temperature and humidity controlled room set to a 12-hour light and dark cycle.…”

Section: Methodsmentioning

confidence: 99%

Saccharomyces cerevisiae-Derived Mannan Does Not Alter Immune Responses to Aspergillus Allergens

LeMessurier

Palipane

Lin

et al. 2018

BioMed Research International

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Severe asthma with fungal sensitization predominates in the population suffering from allergic asthma, to which there is no cure. While corticosteroids are the mainstay in current treatment, other means of controlling inflammation may be beneficial. Herein, we hypothesized that mannan from Saccharomyces cerevisiae would dampen the characteristics of fungal allergic asthma by altering the pulmonary immune responses. Using wild-type and transgenic mice expressing the human mannose receptor on smooth muscle cells, we explored the outcome of mannan administration during allergen exposure on the pathogenesis of fungal asthma through measurement of cardinal features of disease such as inflammation, goblet cell number, and airway hyperresponsiveness. Mannan treatment did not alter most hallmarks of allergic airways disease in wild-type mice. Transgenic mice treated with mannan during allergen exposure had an equivalent response to non-mannan-treated allergic mice except for a prominent granulocytic influx into airways and cytokine availability. Our studies suggest no role for mannan as an inflammatory regulator during fungal allergy.

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“…The origin and nature of the mannan used for coupling is also an important issue to consider. Cell wall mannan from different yeasts differs in structure [ 28 ] and biological activity on DCs [ 32 ]. For example, by using nonoxidized mannan derived from S. cerevisae , no induction of Th17 responses by DCs was detected [ 18 ], in line with other reports [ 33 ] and in contrast to mannan derived from C. albicans [ 33 ].…”

Section: Production and Characterization Of Allergoids Conjugated To mentioning

confidence: 99%

Novel vaccines targeting dendritic cells by coupling allergoids to mannan

et al. 2018

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Allergen-specific immunotherapy (AIT) is the single disease-modifying treatment for allergy. Clinical trials show AIT to be safe and effective for many patients; however, it still faces problems related to efficacy, safety, long treatment duration and low patient adherence. There has been intensive research to develop alternative strategies, including novel administration routes, adjuvants or hypoallergenic molecules. Promising results are reported for some of them, but clinical progress is still moderate. Allergoids conjugated to nonoxidized mannan from Saccharomyces cerevisiae have emerged as a novel concept of vaccine targeting dendritic cells (DCs). Preclinical human and animal models demonstrated that allergoids conjugated to mannan enhance allergen uptake, promote healthy responses to allergens by inducing Th1 and T regulatory (Treg) cells, and show clinical efficacy in veterinary medicine. Dose-finding phase II clinical trials in humans are currently ongoing. We review the current stage of allergoids conjugated to mannan as next generation vaccines for AIT.

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Beneficial Effects of PrebioticSaccharomyces cerevisiaeMannan on Allergic Asthma Mouse Models

Cited by 14 publications

References 35 publications

Questioning Cause and Effect: Children with Severe Asthma Exhibit High Levels of Inflammatory Biomarkers Including Beta-Hexosaminidase, but Low Levels of Vitamin A and Immunoglobulins

Questioning Cause and Effect: Children with Severe Asthma Exhibit High Levels of Inflammatory Biomarkers Including Beta-Hexosaminidase, but Low Levels of Vitamin A and Immunoglobulins

Saccharomyces cerevisiae-Derived Mannan Does Not Alter Immune Responses to Aspergillus Allergens

Novel vaccines targeting dendritic cells by coupling allergoids to mannan

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