Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells

Dong, Xingyou; Zhang, Teng; Liu, Qian; Zhu, Jingzhen; Zhao, Jiang; Li, Jia; Sun, Bishao; Ding, Guolin; Hu, Xiaoyan; Yang, Zhenxing; Zhang, Yuanyuan; Li, Longkun

doi:10.1016/j.mce.2016.03.001

Cited by 49 publications

(42 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the results of the present study, injection of 3 × 10 6 adipose‐derived stem cells into the detrusor and tail vein ameliorated diabetes‐induced bladder dysfunction in the presence of higher blood glucose levels in rats with T2D . In addition, urine‐derived stem cells were shown to improve decreased bladder contractility in diabetic rats . HUCB‐MNCs may exert their beneficial effects, in part, through automatic homing of stem cells and differentiation into SMCs, but paracrine effects seem more likely to have a role in the observed improvements in bladder function .…”

Section: Discussionsupporting

confidence: 89%

“…In the present study, intracavernous injection of HUCB‐MNCs enhanced the smooth muscle content and levels of α‐SMA (a specific marker of SMCs) in the bladder of diabetic rats. Similarly, Dong et al previously reported that treatment with 2 × 10 6 urine‐derived stem cells effectively improved impaired detrusor contractility by ameliorating bladder fibrosis and decreasing α‐SMA protein levels and SMC apoptosis in the diabetic bladder. A hypoxia marker, HIF‐1α plays a significant role in fibrosis by stimulating collagen accumulation and the activation of fibrogenic factors .…”

Section: Discussionmentioning

confidence: 82%

“…Moreover, in different animal models, stem cells administrated via different routes (e.g. tail vein injection, intravenous injection, and local injection into the bladder wall) had beneficial effects on bladder dysfunction . We suggest that the route of administration can result in differences in the effects of stem cells.…”

Section: Discussionmentioning

confidence: 85%

See 2 more Smart Citations

Administration of human umbilical cord blood mononuclear cells restores bladder dysfunction in streptozotocin‐induced diabetic rats

Kaya‐Sezginer

Yilmaz‐Oral

Gür

2019

LUTS

View full text Add to dashboard Cite

Objective This study evaluated the effect of human umbilical cord blood mononuclear cells (HUCB‐MNCs) on bladder dysfunction in streptozotocin (STZ; 35 mg/kg, i.v.)‐induced diabetic rats. Methods Adult male Sprague–Dawley rats (n = 30) were equally divided into three groups: control group, STZ‐diabetic group, and HUCB‐MNC‐treated group (1 × 106 cells). HUCB‐MNCs were isolated by density gradient centrifugation from eight healthy donors and injected into the corpus cavenosum in STZ‐diabetic rats 4 weeks after the induction of diabetes. Studies were performed 4 weeks after HUCB‐MNC or vehicle injection. In vitro organ bath studies were performed on bladder strips, whereas protein expression of hypoxia‐inducible factor (HIF)‐1α, vascular endothelial growth factor (VEGF), and α‐smooth muscle actin (SMA) in the bladder and the ratio of smooth muscle cells (SMCs) to collagen were determined using western blotting and Masson trichrome staining. Results Neurogenic contractions of detrusor smooth muscle strips were 55% smaller in the diabetic group than control group (P < 0.05); these contractions were normalized by HUCB‐MNC treatment. In addition, HUCB‐MNC treatment restored the impaired maximal carbachol‐induced contractile response in detrusor strips in the diabetic group (29%; P < 0.05). HUCB‐MNC treatment improved the KCl‐induced contractile response in the diabetic bladder (68%; P < 0.05), but had no effect on ATP‐induced contractile responses. Increased expression of HIF‐1α and VEGF protein and decreased expression of α‐SMA protein and the SMC/collagen ratio in diabetic rats were reversed by HUCB‐MNC. Conclusion Administration of HUCB‐MNCs facilitates bladder function recovery, which is likely related to downregulation of HIF‐1α expression and attenuation of fibrosis in STZ‐diabetic rats.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 82%

See 1 more Smart Citation

Administration of human umbilical cord blood mononuclear cells restores bladder dysfunction in streptozotocin‐induced diabetic rats

Kaya‐Sezginer

Yilmaz‐Oral

Gür

2019

LUTS

View full text Add to dashboard Cite

show abstract

“…Additionally, recent studies have indicated that the cells isolated from voided urine or urine catheterized from urinary tract have multi-lineage differentiation capability which are highly expandable and posses stem cell features. [1,2,[10][11][12][13] One of the most important results of the present study is that that human urine contains mesenchymal stem cells together with urothelial cells. This result is in compliance with the results other studies published in recent years.…”

Section: Discussionmentioning

confidence: 59%

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Tayhan¹,

Keleş²,

Topçu³

et al. 2017

Turkish Journal of Urology

View full text Add to dashboard Cite

Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods:After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies.Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine-derived cell population. Conclusion:According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.

show abstract

“…The total protein was extracted from the articular cartilage samples. The protein expression of TLR4/NF‐κB signal pathway and inflammatory factors related proteins (IL‐6, IL‐1β, TNF‐α) were used for western blotting as previously reported (Dong et al, ; Zhao et al, ). The primary antibodies, rabbit monoclonal anti‐TLR‐4 (1:2000, CST, #14358), rabbit monoclonal anti‐MyD88 (1:1000, CST, #4283), rabbit monoclonal anti‐Phospho‐IκBα (1:1000, CST, #2859), rabbit monoclonal anti‐NF‐κB (1:3000, CST, #8028), rabbit monoclonal anti‐IL6 (1:4000, CST, #12912), rabbit monoclonal anti‐TNF‐α (1:3000, CST, #11948), rabbit monoclonal anti‐IL‐1β (1:2000, CST, #12703), rabbit monoclonal anti‐β‐actin (1:3000, CST, #4970), horseradish peroxidase‐conjugated goat antirabbit IgG (1:3000 Zhongshan Inc), were incubated.…”

Section: Methodsmentioning

confidence: 99%

Curcumin reduces inflammation in knee osteoarthritis rats through blocking TLR4 /MyD88/NF‐κB signal pathway

Zhang

Zeng

2019

Drug Development Research

View full text Add to dashboard Cite

Preclinical Research & Development Curcumin has been shown to possess a series of beneficial effects, such as antiinflammatory, antioxidant, analgesic, and promoting healing. However, the effect and relative mechanism of curcumin on knee osteoarthritis (OA) have not been elucidated. The aim of this study is to explore the protective effect of curcumin on monosodium iodoacetate (MIA)‐induced OA. Forty‐eight rats were randomized into four experimental groups: control group, OA group, OA + PBS group, and OA + curcumin group, respectively. A single intraarticular injection of MIA was applied to establish the rat model of knee OA. Hematoxylin–eosin staining was used to evaluate histological changes of knee joint. The paw withdrawal threshold was collected and the expression of synovial fluid cytokine levels was measured by ELISA. The protein expression of TRL‐4, MyD88, p‐IκBα, NF‐κB, TNF‐α, IL‐1β, and IL6 was measured by western blot. Treating with curcumin can significantly reduce joint diameter and Mankin's score, and increase the paw withdrawal threshold. The expression of synovial fluid inflammatory biomarkers, IL‐6, IL‐1β, and TNF‐α in the OA + curcumin group were lower than that in OA and OA + PBS group. The protein expression of the TLR4 receptor was increased in the OA, OA + PBS, and OA + curcumin group compared to the control group. However, curcumin treatment can significantly decrease the expression of MyD88, p‐IκBα, NF‐κB, TNF‐α, IL‐1β, and IL6 in OA + curcumin group. These findings may indicate that curcumin could block TLR4/NF‐κB signal pathway, and reduce inflammation level to prevent knee wound in OA rats. Curcumin may be a feasible kind of medicament in the treatment of knee OA.

show abstract

Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells

Cited by 49 publications

References 40 publications

Administration of human umbilical cord blood mononuclear cells restores bladder dysfunction in streptozotocin‐induced diabetic rats

Administration of human umbilical cord blood mononuclear cells restores bladder dysfunction in streptozotocin‐induced diabetic rats

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Curcumin reduces inflammation in knee osteoarthritis rats through blocking TLR4 /MyD88/NF‐κB signal pathway

Contact Info

Product

Resources

About