Beneficial impact of exogenous arginine, cysteine and methionine on postharvest senescence of broccoli

Kha

J. Food Process. Preserv.

et al. 2021

Response surface methodology was used to optimize hydrolysis conditions of collagen from yellowfin tuna skin (Thunnus albacares) for maximizing the degree of hydrolysis (DH) and nitrogen recovery efficiency (NR). The results showed that the optimum hydrolysis parameters were at 54.7°C and pH 8 with 0.034 AU/g alcalase concentration for 5.2 hr. The predicted DH and NR under optimal conditions were validated and confirmed as 24.51% and 96.35%, respectively. The obtained collagen hydrolysate (CH) was rich in proline, glycine, methionine, and hydroxyproline residues. The antioxidation and antifreezing activity of CH were identified as 2,2‐diphenyl‐1‐picrylhydrazyl radical activity (IC50 77.04 µg/ml), and hydroxyl radical activity (IC50 36.59 µg/ml). It also exhibited antifreezing property and had the inhibition rate of peroxide value of oil up to 73.53%. The results suggested that CH from yellowfin tuna skin could be used in the production of functional food products or other related industries. Practical applications Due to its low solubility, collagen has limited applications in industry. This study aimed to optimize the hydrolysis conditions of the collagen from yellowfin tuna skin in order to apply in industry. The collagen hydrolysate which is rich in proline, glycine, methionine, and hydroxyproline residues has excellent antioxidant, antifreezing, and antioxidation properties. Collagen hydrolysate can be used in the production of food and functional food products or other related industries due to excellent antioxidant and antifreezing properties, and good inhibition rate of oil oxidation.

Section: Resultsmentioning

confidence: 99%

Optimization of enzymatic hydrolysis of collagen from yellowfin tuna skin ( Thunnus albacares ) by response surface methodology and properties of hydrolyzed collagen

Kha

J. Food Process. Preserv.

et al. 2021

Oxidative Medicine and Cellular Longevity

“…Methionine is involved in destruction of membrane lipids produced by oxidative free radicals through multiple channels of oxidation thus protecting membrane-containing structures such as cells and mitochondria [36]. Downregulation of cysteine and methionine metabolism during degeneration process leads to decline of the antioxidant function in the internal environment of the IVD [37]. Peroxidation of tissue membranes promotes degeneration of the IVD.…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Glycine‐Serine‐Threonine Metabolic Axis Delays Intervertebral Disc Degeneration through Antioxidant Effects: An Imaging and Metabonomics Study

Liu

Yang

et al. 2021

Although intervertebral disc degeneration (IDD) can be described as different stages of change through biological methods, this long and complex process cannot be defined in stages by single or simple combination of biological techniques. Under the background of the development of nuclear magnetic resonance (NMR) technology and the emerging metabonomics, we based on animal models and expanded to the study of clinical human degeneration models. The characteristics of different stages of IDD were analyzed by omics. Omics imaging combined with histology, cytology, and proteomics was used for screening of the intervertebral disc (IVD) of research subjects. Furthermore, mass spectrometry nontargeted metabolomics was used to explore profile of metabolites at different stages of the IDD process, to determine differential metabolic pathways and metabolites. NMR spectroscopy was used to qualitatively and quantitatively identify markers of degeneration. NMR was combined with mass spectrometry metabolomics to explore metabolic pathways. Metabolic pathways were determined through protein molecular biology and histocytology of the different groups. Distinguishing advantages of magnetic resonance spectroscopy (MRS) for analysis of metabolites and effective reflection of structural integrity and water molecule metabolism through diffusion tensor imaging (DTI) were further used to verify the macrometabolism profile during degeneration. A corresponding model of in vitro metabolomics and in vivo omics imaging was established. The findings of this study show that a series of metabolic pathways associated with the glycine-serine-threonine (Gly-Ser-Thr) metabolic axis affects carbohydrate patterns and energy utilization efficiency and ultimately delays disc degeneration through antioxidant effects.

“…Respiration is an indication of the tissue metabolic rate and the major factor that contributes to the deterioration and loss of quality of plant produce during postharvest storage [33]. Chive leaves, with a very high respiratory rate, suffer senescence very quickly after harvest.…”

Section: -Mcp Enhances Antioxidant Capacity and Delays Senescence In Postharvest Chivesmentioning

confidence: 99%

1-Methylcyclopropene Preserves the Quality of Chive (Allium schoenoprasum L.) by Enhancing Its Antioxidant Capacities and Organosulfur Profile during Storage

Dai

Lü

Yang

et al. 2021

Foods

The quality, antioxidant capacities, and organosulfur profile of chives (Allium schoenoprasum L.) treated with 1-methylcyclopropene (1-MCP) during storage were investigated in this study. The 1-MCP treatment (100 μL/L, fumigation 12 h at 20 °C) effectively inhibited tissue respiration and H2O2 production, enhanced the ascorbic acid (ASA) and glutathione (GSH) content, and promoted the activity of antioxidant enzymes (superoxide dismutase SOD, Catalase CAT, and ascorbic peroxidase APX) during the 5-day storage period at 20 °C. The result further showed that the 1-MCP treatment inhibited chlorophyll degradation, alleviated cell membrane damage, and delayed the chive senescence, with the yellowing rate being reduced by 67.8% and 34.5% in the 1-MCP treated chives on days 4 and 5 of storage at 20 °C, respectively. The free amino acid content of the chive was not affected by the 1-MCP treatment at 20 °C. However, the senescence rate of the chive was not reduced by the 1-MCP treatment when stored at 3 °C. The liquid chromatography data further showed that the 1-MCP treatment induced a 15.3% and 13.9% increase in the isoalliin and total S-alk(en)ylcysteine sulfoxides (ACSOs) content of the chive on day 2 at 20 °C, respectively. Furthermore, there was a strong positive correlation between ACSOs content and CAT/APX activity, indicating that ACSOs probably played a key role in enhancing the antioxidant capacities of the chive during storage at 20 °C. Thus the study efficiently demonstrates that 1-methylcyclopropene preserves the quality of chive (Allium schoenoprasum L.) by enhancing its antioxidant capacities and organosulfur profile during storage.