Benefits of high nitrogen fertilizer on nitrogen metabolism, nitrogen transfer rate, root system architecture and grain yield of wheat (Triticum aestivum L.) under water deficit at heading stage

He, Mengdi; Jiang, Yanjie; Liu, Lulu; Zhong, Xuanbo; Zhao, Yun; Ma, Wujun; Tang, Guixiang

doi:10.1007/s11738-022-03460-0

Cited by 6 publications

(2 citation statements)

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“…Superoxide dismutase (SOD, EC 1.15.1.1) was assayed by the nitrogen blue tetrazolium (NBT) method [ 56 ]. One unit of SOD activity was the amount of enzyme required to cause 50% inhibition of the NBT reduction at 560 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Ascorbate peroxidase (APX, EC 1.11.1.11) activity was determined by the method of Nakano and Asada (1981) [59]. Malondialdehyde (MDA) was determined by the thiobarbituric acid method [56]. Three replicates were set up for each treatment for each sample for each assay and the mean of the three replicates was taken.…”

Section: Measurement Of Antioxidant Enzyme Activities and Malondialde...mentioning

confidence: 99%

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Physiological Regulation of Photosynthetic-Related Indices, Antioxidant Defense, and Proline Anabolism on Drought Tolerance of Wild Soybean (Glycine soja L.)

Lin,

Zhang,

Wang

et al. 2024

Plants

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Wild soybean (Glycine soja L.), drought-tolerant cultivar Tiefeng 31 (Glycine max L.), and drought-sensitive cultivar Fendou 93 (Glycine max L.) were used as materials to investigate the drought tolerance mechanism after 72 h 2.5 M PEG 8000 (osmotic potential −0.54 MPa)-simulated drought stress at the seedling stage. The results indicated that the leaves of the G. soja did not wilt under drought stress. However, both the drought-tolerant and drought-sensitive cultivated soybean cultivars experienced varying degrees of leaf wilt. Notably, the drought-sensitive cultivated soybean cultivars exhibited severe leaf wilt after the drought stress. Drought stress was determined to have a significant impact on the dry matter of the above-ground part of the drought-sensitive cultivar Fendou 93, followed by the drought-tolerant cultivar Tiefeng 31, with the lowest reduction observed in G. soja. Furthermore, the presence of drought stress resulted in the closure of leaf stomata. G. soja exhibited the highest proportion of stomatal opening per unit area, followed by the drought-tolerant cultivar Tiefeng 31, while the drought-sensitive cultivar Fendou 93 displayed the lowest percentage. Photosynthesis-related indexes, including photosynthetic rate, intercellular CO2, transpiration rate, and stomatal conductance, decreased in Fendou 93 and Tiefeng 31 after drought stress, but increased in G. soja. In terms of the antioxidant scavenging system, lower accumulation of malondialdehyde (MDA) was observed in G. soja and Tiefeng 31, along with higher activities of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) to counteract excess reactive oxygen species and maintain cell membrane integrity. In contrast, the drought-sensitive cultivar Fendou 93 had higher MDA content and higher activities of ascorbate peroxidase (APX, EC 1.11.1.11) and peroxidase (POD, 1.11.1.7). G. soja and Tiefeng 31 also exhibited less accumulation of osmolytes, including soluble sugar, soluble protein, and free proline content. The activities of δ-OAT, ProDH, and P5CS, key enzymes in proline anabolism, showed an initial increase under drought stress, followed by a decrease, and then an increase again at the end of drought stress in G. soja. Before drought stress, Tiefeng 31 had higher activities of ProDH and P5CS, which decreased with prolonged drought stress. Fendou 93 experienced an increase in the activities of δ-OAT, ProDH, and P5CS under drought stress. The δ-OAT gene expression levels were up-regulated in all three germplasms. The expression levels of the P5CS gene in Fendou 93 and Tiefeng 31 were down-regulated, while G. soja showed no significant change. The expression of the P5CR gene and ProDH gene was down-regulated in Fendou 93 and Tiefeng 31, but up-regulated in G. soja. This indicates that proline content is regulated at both the transcription and translation levels.

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Section: Methodsmentioning

confidence: 99%