Benzalkonium Chloride Sterilization of Nonwoven Fibrous Scaffolds for
Astrocyte Culture

Ng, Robin; Gurm, Jesse Singh; Yang, Shang‐Tian

doi:10.2174/1874070700903010073

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…3.4.1.1 UV irradiation. Although UV irradiation is usually ineffective for scaffold sterilization, 107 it can degrade nanotextured materials and thus could be used to fabricate microporous scaffolds from electrospun nanofibers. 108 By masking the nanofibers with a porous mesh and subjecting them to UV irradiation, the porosity and pore size of the scaffolds can be controlled by controlling the pore size of the porous mask.…”

Section: 41mentioning

confidence: 99%

Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

et al. 2012

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Section: 41mentioning

confidence: 99%

Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

et al. 2012

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Novel 3D scaffold with enhanced physical and cell response properties for bone tissue regeneration, fabricated by patterned electrospinning/electrospraying

Hejazi

Mirzadeh

2016

J Mater Sci: Mater Med

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Developing three dimensional scaffolds mimicking the nanoscale structure of native extracellular matrix is a key parameter in tissue regeneration. In this study, we aimed to introduce a novel 3D structures composed of nanofibers (NF) and micro particles (MP) and compare their efficiency with 2D nanofibrous scaffold. The conventional nanofibrous PCL scaffolds are 2D mats fabricated by the electrospinning technique, whereas the NF/MP and patterned NF/MP PCL scaffolds are three dimensional structures fabricated by a modified electrospinning/electrospraying technique. The mentioned method was carried out by varying the electrospinning solution parameters and use of a metal mesh as the collector. Detailed fabrication process and morphological properties of the fabricated structures is discussed and porosity, pore size and PBS solution absorption value of the prepared structures are reported. Compared with the 2D structure, 3D scaffolds possessed enhanced porosity and pore size which led to the significant increase in their water uptake capacity. In vitro cell experiments were carried out on the prepared structures by the use of MG-63 osteosarcoma cell line. The fabricated 3D structures offered significantly increased cell attachment, spread and diffusion which were confirmed by SEM analysis. In vitro cytocompatibility assessed by MTT colorimetric assay indicated a continuous cell proliferation over 21 days on the innovative 3D structure, while on 2D mat cell proliferation stopped at early time points. Enhanced osteogenic differentiation of the seeded MG-63 cells on 3D scaffold was confirmed by the remarkable ALP activity together with increased and accelerated calcium deposition on this structure compared to 2D mat. Massive and well distributed bone minerals formed on patterned 3D structure were shown by EDX analysis. In comparison between NF/MP quasi-3D and Patterned NF/MP 3D scaffolds, patterned structures proceeded in all of the above properties. As such, the innovative Patterned NF/MP 3D scaffold could be considered as a proper bone graft substitute for bone tissue regeneration.

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Benzalkonium Chloride Sterilization of Nonwoven Fibrous Scaffolds for Astrocyte Culture

Cited by 2 publications

References 35 publications

Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

Novel 3D scaffold with enhanced physical and cell response properties for bone tissue regeneration, fabricated by patterned electrospinning/electrospraying

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