Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

Ng, Robin; Zang, Ru; Yang, Kevin; Liu, Ning; Yang, Shang‐Tian

doi:10.1039/c2ra21085a

Cited by 126 publications

(106 citation statements)

References 140 publications

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“…Therefore, using a biosensor to measure the in situ oxygen level, as shown in Figure 2, would be useful to fully understand the effect of oxygen in stem cell fate decision. Perfusion improved tissue architecture of engineered cardiac muscle and increased matrix synthesis in engineered chondrocytes [73,74] Agitation preserved Oct-4 expressing cells during PSC differentiation [82,83] for culturing and engineering stem cells [12,[34][35][36][37] . The material properties including surface topology and stiffness or tensile strength have profound effects on cell adhesion, proliferation, and the differentiated cellular function [38] .…”

Section: Control Of Oxygen Tension In Bioreactorsmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as "niches", to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix (synthetic and decellularized), paracrine/ autocrine signaling and physical forces (i.e. , mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general, bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.

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Section: Control Of Oxygen Tension In Bioreactorsmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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“…On the other hand, they lack nanotextured topographical features, which are needed for the scaffold to mimic like native ECM. 49 Further, successes in directing cells towards certain cellular morphologies, functions, and differentiations have been studied with diverse types of nanofibers. 49,50 Recent studies of tissue engineering have paid great attention to combining these two scaffold features towards the development of ideal scaffold fabrication.…”

Section: Fbg and Fbn Micro/nanofiber As Scaffoldsmentioning

confidence: 99%

“…49 Further, successes in directing cells towards certain cellular morphologies, functions, and differentiations have been studied with diverse types of nanofibers. 49,50 Recent studies of tissue engineering have paid great attention to combining these two scaffold features towards the development of ideal scaffold fabrication. Recently, we developed nanofiber-aligned Fbg microfibers, which showed a higher rate of biomolecule encapsulation and proliferation than other highly porous and less pores Fbg microfibers.…”

Section: Fbg and Fbn Micro/nanofiber As Scaffoldsmentioning

confidence: 99%

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Rajangam¹,

An²

2013

IJN

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Over the past two decades, many types of natural and synthetic polymer-based micro-and nanocarriers, with exciting properties and applications, have been developed for application in various types of tissue regeneration, including bone, cartilage, nerve, blood vessels, and skin. The development of suitable polymers scaffold designs to aid the repair of specific cell types have created diverse and important potentials in tissue restoration. Fibrinogen (Fbg)-and fibrin (Fbn)-based micro-and nanostructures can provide suitable natural matrix environments. Since these primary materials are abundantly available in blood as the main coagulation proteins, they can easily interact with damaged tissues and cells through native biochemical interactions. Fbg-and Fbn-based micro and nanostructures can also be consecutively furnished/ or encapsulated and specifically delivered, with multiple growth factors, proteins, and stem cells, in structures designed to aid in specific phases of the tissue regeneration process. The present review has been carried out to demonstrate the progress made with micro and nanoscaffold applications and features a number of applications of Fbg-and Fbn-based carriers in the field of biomaterials, including the delivery of drugs, active biomolecules, cells, and genes, that have been effectively used in tissue engineering and regenerative medicine.

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“…(Curtis and Wilkinson 1997, Sarna et al 2009, Flemming et al 1999, Andersson et al 2003, Matsuzaka et al 2003, Recknor et al 2004, Miller et al 2007, Falconnet et al 2006, Martinez et al 2009, Lamers et al 2010, Zhang and Webster 2012, Hsu et al 2005 These textured biomaterials may mimic in vivo microenvironments, and thus enable modelling of cell-cell and cell-extracellular matrix interactions and modulation of cell-surface interactions. (Ng et al 2012, Craighead et al 1998, Martinez et al 2009, Dalby et al 2004 Although there are numerous studies on textured materials, the complexity of their role in controlling cell interactions and cell responses is still yet not fully understood and hence is impossible to predict without carrying out the individual cell studies. Also the response mechanisms of different cell lines to textured surfaces are not well defined.…”

Section: Introductionmentioning

confidence: 99%

The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

et al. 2016

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(2016) The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment. Nanotechnology, 27 (25) A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. KEYWORDS Nanotexturing, human ovarian cancer cell, poly(lactic-co-glycolic acid), films ABSTRACT In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane (PDMS) mold against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. 2Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSC) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to nontextured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 hour time points.

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Three-dimensional fibrous scaffolds with microstructures and nanotextures for tissue engineering

Cited by 126 publications

References 140 publications

Engineering stem cell niches in bioreactors

Engineering stem cell niches in bioreactors

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

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