Benzo(a)pyrene disrupts mouse preimplantation embryo development

Zhan, Shaoquan; Zhang, Xiya; Cao, Sheng‐Li; Huang, Junjiu

doi:10.1016/j.fertnstert.2014.11.013

Cited by 29 publications

(8 citation statements)

References 70 publications

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“…However, the effects of B[a]P at a level approximate to physiologic concentrations remain unclear. In the present work, TSCC cells underwent long-term exposure to a 5 nM of B[a]P (Huang et al, 2013;Zhan, Zhang, Cao, & Huang, 2015). In light of the accumulative effect of ingestion and inhalation of cigarette smoke, 50 nM of B [a]P was also used to analyze the role of B[a]P on TSCC.…”

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confidence: 99%

Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

et al. 2020

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Tongue squamous cell carcinoma (TSCC) is the most common head and neck cancer and accounts for 1%-2% of all human malignancies (Erickson, 2017). Many studies have indicated that tobacco smoking is an important risk factor for TSCC (Hashibe et al., 2009). Smoking is related to a poor prognosis in TSCC patients by decreasing survival rate and increasing the risk of recurrence, as well by increasing the incidence of secondary cancers (Warren, Kasza, Reid, Cummings, & Marshall, 2013). Smoking during cancer treatment is associated with

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confidence: 99%

Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

et al. 2020

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“…Women exposed to mainstream smoke had signi cantly higher levels of BaP (1.32 ± 0.68ng/ml, about 5nM) in follicular uid compared to non-smoking (0.03 ± 0.01ng/ml) ones [20]. Our previous study showed that this internal exposure concentration (5nM) of BaP can seriously disrupt mouse preimplantation embryo development [21].…”

Section: Introductionmentioning

confidence: 84%

Nicotinic acid protects germinal vesicle oocyte meiosis in mice and humans against toxicity of benzo(a)pyrene during maturation

Gao

Zhan

et al. 2023

Preprint

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Background Approximately 5 nM -7 nM internal exposure concentration of BaP was detected in women who mainstream smoke or suffering assisted reproductive failure. In this study, we evaluated the effects of benzo(a)pyrene (BaP) on mouse and human germinal vesicle (GV) oocyte maturation in 5 nM BaP. Then, we tested whether nicotinic acid (NA) could restore GV meiotic failure caused by the BaP or not during in vitro maturation (IVM)?Methods Clinically discarded GV oocytes from young women (aged < 35 years) undergoing intracytoplasmic sperm injection (ICSI) / in vitro fertilization (IVF) and GV oocytes from 6–8 weeks old female CD1 mice were used. Oocyte quality was estimated by GV oocyte maturation, morphological observation, and mitochondrial functions. The subcellular structures were further evaluated using immunostaining, fluorescent intensity quantification or western blot to analyze spindle organization, chromosome arrangement, actin polymerization, mitochondrial distribution, DNA damage and the Sirt1 protein level during mouse oocyte meiotic.Results We found 5 nM/50 nM BaP exposure significantly reduced first polar body extrusion during mouse GV oocytes maturation. Sirt1 protein expression decreased after BaP treatment in mouse oocytes. Moreover, BaP exposure disorganized spindle and chromosome arrangement, disrupted cortical actin cap, impaired mitochondrial redistribution, and caused DNA damage in IVM metaphase II (MII) mouse oocytes. Importantly, NA supplementation (15µM) increased Sirt1 expression and significantly rescued most of the abnormal effects. We then explored the effect of 5 nM BaP on human GV oocytes, a concentration close to that in human ovarian follicular fluid, and found that BaP caused GV meiotic failure by increasing mitochondrial membrane potential and markedly elevating reactive oxygen species (ROS) levels. Finally, we showed that 15 µM NA supplementation partially rescued human GV oocytes from the toxicity of 5 nM BaP during IVM.Conclusions Collectively, our study indicated that internal exposure concentrations of BaP could seriously disrupt GV oocyte IVM and caused GV meiotic defects in both mouse and human. NA partially protected GV oocyte meiosis against toxicity of BaP during IVM.

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“…The collection of zygotes was performed as previously described [18]. CD1 female mice were super-ovulated with 5 IU PMSG (367222, Calbiochem) and 5 IU hCG (230734, Calbiochem) for 46 h and used for breeding.…”

Section: Methodsmentioning

confidence: 99%

The long non-coding RNA Snhg3 is essential for mouse embryonic stem cell self-renewal and pluripotency

Shi

et al. 2019

Stem Cell Res Ther

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Background Small nucleolar RNA host gene 3 (Snhg3) is a long non-coding RNA (lncRNA) that was shown to participate in the tumorigenesis of certain cancers. However, little is known about its role in embryonic stem cells (ESCs). Methods Here, we investigated the role of Snhg3 in mouse ESCs (mESCs) through both loss-of-function (knockdown) and gain-of-function (overexpression) approaches. Alkaline phosphatase staining, secondary colony formation, propidium iodide staining, western blotting, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to access self-renewal capacity, whereas immunofluorescence, qRT-PCR, and embryoid body formation were performed to examine pluripotency. In addition, the effect of Snhg3 on mouse embryonic development was determined based on the morphological changes, blastocyst rate, and altered pluripotency marker (Nanog, Oct4) expression. Moreover, the relationship between Snhg3 and key pluripotency factors was evaluated by chromatin immunoprecipitation qPCR, qRT-PCR, subcellular fractionation, and RNA immunoprecipitation. Finally, RNA pull-down and mass spectrometry were applied to explore the potential interacting proteins of Snhg3 in mESCs. Results We demonstrated that Snhg3 is essential for self-renewal and pluripotency maintenance in mESCs. In addition, Snhg3 knockdown disrupted mouse early embryo development. Mechanistically, Snhg3 formed a positive feedback network with Nanog and Oct4, and 126 Snhg3-interacting proteins were identified in mESCs. Conclusions Snhg3 is essential for mESC self-renewal and pluripotency, as well as mouse early embryo development. Electronic supplementary material The online version of this article (10.1186/s13287-019-1270-5) contains supplementary material, which is available to authorized users.

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Benzo(a)pyrene disrupts mouse preimplantation embryo development

Cited by 29 publications

References 70 publications

Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

Nicotinic acid protects germinal vesicle oocyte meiosis in mice and humans against toxicity of benzo(a)pyrene during maturation

The long non-coding RNA Snhg3 is essential for mouse embryonic stem cell self-renewal and pluripotency

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