Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

Zhang, Linbao; Liu, Xiaoli; You, Liu; Zhou, Di; Wang, Qing; Li, Fēi; Cong, Ming; Li, Lianzhen; Zhao, Jianmin; Chen, Lingxin; Yu, Junbao; Wu, Huifeng

doi:10.1016/j.etap.2011.05.006

Cited by 35 publications

(55 citation statements)

References 53 publications

(53 reference statements)

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“…Polar metabolites were extracted from the oyster tissues by a modified extraction protocol using methanol/chloroform (Zhang et al, 2011). Briefly, the oyster tissue (~100 mg) was homogenized using a high throughput homogenizer (Precellys 24, Bertin Technologies, France) and extracted in 4 mL/g of methanol, 0.85 mL/g of water and 2 mL/g of chloroform.…”

Section: H Nmrmentioning

confidence: 99%

A metabolomic study on the biological effects of metal pollutions in oysters Crassostrea sikamea

Wang

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oMetal pollution has become a great threat to organisms in the estuaries in South China. In the present study, the oysters Crassostrea sikamea were collected from one clean (Jiuzhen) and five metal polluted sites (Baijiao, Fugong, Gongqian, Jinshan and Songyu). The tissue metal concentrations in oysters indicated that the five metal sites were polluted by several metals, including Cr, Ni, Co, Cu, Zn, Ag, Cd and Pb with different patterns. Especially, Cu and Zn were the major contaminants in Baijiao, Fugong and Jinshan sites. The metabolic responses in oysters C. sikamea indicated that the metal pollutions in BJ, FG, JS and SY sites induced disturbances in osmotic regulation and energy metabolism via different metabolic pathways. However, the metal pollution in GQ site mainly influenced the osmotic regulation in the oysters C. sikamea. This study demonstrates that NMR-based metabolomics is useful to characterize metabolic responses induced by metal pollution.

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Section: H Nmrmentioning

confidence: 99%

A metabolomic study on the biological effects of metal pollutions in oysters Crassostrea sikamea

Wang

et al. 2016

Marine Pollution Bulletin

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“…Polar metabolites in clam tissues (n ¼ 8 for each treatment) were extracted by the modified extraction protocol as described previously [15]. Briefly, the digestive gland tissue (~100 mg wet weight) was homogenized and extracted in 4 mL/g of methanol, 5.25 mL/g of water and 2 mL/g of chloroform.…”

Section: Metabolite Extractionmentioning

confidence: 99%

“…Metabolite extracts of digestive gland tissues from clams were analyzed on a Bruker AV 500 NMR spectrometer performed at 500.18 MHz (at 25 C) as described previously [15]. All 1 H NMR spectra were phased, baseline-corrected, and calibrated (TSP at 0.0 ppm) manually using TopSpin (version 2.1, Bruker).…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…All one dimensional 1 H NMR spectra were converted to a data matrix using the custom-written ProMetab software in Matlab version 7.0 (The MathsWorks, Natick, MA) [15]. Each spectrum was segmented into bins with a width of 0.005 ppm between 0.2 and 10.0 ppm.…”

Section: Spectral Pre-processing and Multivariate Analysismentioning

confidence: 99%

“…The total spectral area of the remaining bins was normalized to unity to facilitate the comparison between the spectra. All the NMR spectra were generalized log transformed (glog) with a transformation parameter l ¼ 1.0 Â 10 À8 to stabilize the variance across the spectral bins and to increase the weightings of the less intense peaks [15]. As described in details in our previous work, data were mean-centered before multivariate data analysis.…”

Section: Spectral Pre-processing and Multivariate Analysismentioning

confidence: 99%

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Comparative investigations on the biological effects of As (III) and As (V) in clam Ruditapes philippinarum using multiple biomarkers

Wang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c tInorganic arsenic is a known pollutant with two chemical forms, arsenite (As (III)) and arsenate (As (V)), in marine environment. Clam Ruditapes philippinarum is an important fishery species along the Bohai coast. In this study, the biological effects induced by the two arsenic chemical forms (arsenite and arsenate) were compared using multiple biochemical indices in the digestive glands of clam R. philippinarum. The production of reactive oxygen species, antioxidant enzyme activities and metabolic responses exhibited that both As (III) and As (V) induced immune, oxidative and osmotic stresses in clam digestive glands. The differential metabolic biomarkers, histidine and taurine, indicated the differential responsive mechanisms in osmotic regulation in clam digestive glands. In addition, both arsenic treatments enhanced the anaerobiosis metabolism in clam digestive glands. Overall, this work illustrated that arsenite and arsenate induced similar biological effects in clams, which might be accounted for the biological transformation of arsenate to arsenite in clams.

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Environmental Metabolomics of Aquatic Organisms

Størseth

Hammer

1996

eMagRes

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Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (1H NMR) based metabolomics

Cited by 35 publications

References 53 publications

A metabolomic study on the biological effects of metal pollutions in oysters Crassostrea sikamea

A metabolomic study on the biological effects of metal pollutions in oysters Crassostrea sikamea

Comparative investigations on the biological effects of As (III) and As (V) in clam Ruditapes philippinarum using multiple biomarkers

Environmental Metabolomics of Aquatic Organisms

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