�ber eine ?chemorezeptive? Sinneszelle in der Trachea der Ratte

Luciano, L.; Reale, E.; Rüska, H.

doi:10.1007/bf00328847

Cited by 104 publications

(48 citation statements)

References 76 publications

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“…In the past few years there has been growing evidence in favor of a sensory function of brush cells (Kugler et al 1994;Höfer and Drenckhahn 1998), as had earlier been suggested (Rhodin and Dalhamn 1956;Silva 1966;Luciano et al 1968). The peculiarities in the composition of the glycoconjugates in the apical membrane, as detected in the present study, further support this idea, because peculiarities in the lectin binding properties have previously been described for sensory cells of other organs.…”

Section: Discussionsupporting

confidence: 89%

Brush Cells of the Mouse Intestine Possess a Specialized Glycocalyx as Revealed by Quantitative Lectin Histochemistry: Further Evidence for a Sensory Function

Gebhard

Gebert

1999

J Histochem Cytochem.

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SUMMARY Brush cells occur in the epithelium of the small intestine and in various other epithelia of endodermal origin. Ultrastructural and histochemical characteristics suggest that they represent sensory cells. Because the apical membrane of brush cells might be involved in and specialized for (chemo-)receptive functions, we investigated the composition of the glycocalyx and compared it with that of enterocytes. Ultrathin sections of murine small intestine were labeled with a panel of eight lectins. Their binding sites in the brush border and on vesicles of the apical cytoplasm were detected by colloidal gold and quantified using image analysis. The glycocalyx of brush cells contained significantly higher amounts of L -fucose residues than that of enterocytes, as detected by the lectins UEA-I and LTA. In contrast, most of the other lectins bound more avidly to the glycocalyx of enterocytes. The cytoplasmic vesicles closely resembled the apical membrane in their labeling pattern. Quantitation of the brush cells' distribution revealed that the epithelia of the Peyer's patches contained 10-fold higher numbers of brush cells than the small intestinal mucosa distant from lymphoid tissue. We conclude that brush cells possess a glycocalyx with a specialized composition and differ significantly from enterocytes. Because similar peculiarities of the apical membrane have previously been described for sensory cells of the olfactory and gustatory organs, this study provides further evidence in favor of a sensory function of brush cells.

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Section: Discussionsupporting

confidence: 89%

Brush Cells of the Mouse Intestine Possess a Specialized Glycocalyx as Revealed by Quantitative Lectin Histochemistry: Further Evidence for a Sensory Function

Gebhard

Gebert

1999

J Histochem Cytochem.

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“…This same function was also assumed for the BCs of the epithelia of the gastrointestinal tract and their appendages in which, although intraepithelial nerve elements are missing, successive findings provided additional support that BCs may function as receptor cells (Luciano et al 1981(Luciano et al ,1993Reale 1990,1997;Kugler et al 1994). Even in earlier detailed descriptions after thin-section transmission electron microscopy (Luciano et al 1968;Luciano and Reale 1969), two main characteristics of BCs were evident: the prominent microvillous brush border (from which they received their name; Rhodin and Dalhamn 1956) and the architecture of the cytoskeleton. The three building elements F-actin, microtubules, and intermediate filaments are uncommonly abundant and display an extremely ordered spatial arrangement and distribution in the cytoplasm (Luciano and Reale 1997 (Kasper et al 1994).…”

mentioning

confidence: 56%

“…The task of BCs is not known at the present. In the trachea, because of the morphological resemblance of fully differentiated BCs to receptor cells and above all because of the presence of afferent synaptic contacts between postnatal developing BCs and intraepithelial nerve endings, a sensory function was proposed (Luciano et al 1968). This same function was also assumed for the BCs of the epithelia of the gastrointestinal tract and their appendages in which, although intraepithelial nerve elements are missing, successive findings provided additional support that BCs may function as receptor cells (Luciano et al 1981(Luciano et al ,1993Reale 1990,1997;Kugler et al 1994).…”

mentioning

confidence: 99%

Brush Cells of Rodent Gallbladder and Stomach Epithelia Express Neurofilaments

Luciano

Groos

Reale

2003

J Histochem Cytochem.

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S U M M A R Y It has been suggested that brush cells (BCs), a distinct type of cell occurring in various epithelia of the respiratory and gastrointestinal tracts, may function as receptor cells. The major characteristics of BCs are a prominent brush border and an unusually highly ordered arrangement of cytoskeletal elements (F-actin, microtubules, and intermediate filaments). In this study we aimed to characterize the nature of the intermediate filaments in BCs by light and electron microscopic immunostaining. Gallbladder and stomach specimens from mice and rats, respectively, were fixed in various solutions, embedded either in paraffin or epoxy resin, and processed for immunodetection. Commercially available, well-characterized antibodies against neurofilaments, peripherin, and cytokeratin peptide 18 were used. The polyclonal antiserum cocktail to neurofilaments was applied as a supplement in a double-labeling procedure with anti-actin and anti-cytokeratin 18 antibodies. The results demonstrate that the BCs of both organs express two types of intermediate filaments, i.e., neurofilaments and cytokeratin 18 filaments, and that these have a compartmentalized distribution in the cytoplasm. BCs do not express peripherin

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“…Although they have been identified throughout the conducting airway of many species, their presence is infrequent and has not been convincingly shown in humans (Meyrick and Reid, 1968;Jeffery and Reid, 1975). While their function is not well-defined, some speculated functions include roles in periciliary fluid absorption (Jeffery, 1987), chemoreception (Luciano et al, 1968) and ciliogenesis (Rhodin and Dalhamn, 1956). …”

Section: Brush Cellsmentioning

confidence: 99%