Berberine alleviates the cerebrovascular contractility in streptozotocin-induced diabetic rats through modulation of intracellular Ca2+ handling in smooth muscle cells

Ma, Yihui; Zhang, Yin-Bin; Bai, Yuxiang; Dai, Zhijun; Liang, Liang; Liu, Mei; Xie, Man‐Jiang; Guan, Huaijin

doi:10.1186/s12933-016-0382-9

Cited by 31 publications

(32 citation statements)

References 32 publications

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“…100 mg/kg/day berberine for 8 weeks markedly decreased the blood glucose and increased the body weight of diabetic rats, respectively, which is well in accordance with previous reports (Wu et al 2012, Moghaddam et al 2013, Ma et al 2016. Chronic administration of 200 mg/ kg/day berberine for 8 weeks significantly decreased blood glucose in Diabetic rats and CON rats (Table 1).…”

Section: Physical Characteristics Of Experimental Animalssupporting

confidence: 92%

“…As shown in Fig. 8A, extracellular application of high glucose (20 mM d-glucose for 2-7 min) (Ma et al 2016) obviously induced the contraction. When the contraction was stable, acute extracellular application of 10 µM berberine markedly induced the relaxation in the presence of 20 mM d-glucose.…”

Section: Acute Application Of Berberine Directly Induced the Relaxatimentioning

confidence: 76%

“…When the contraction was stable, acute extracellular application of 10 µM berberine markedly induced the relaxation in the presence of 20 mM d-glucose. The dose of acute extracellular application of 10 µM berberine was used in this study according to our pre-experiments and our previous reports (Ma et al 2016). Subsequently, extracellular application of 100 nM IBTX, the specific blocker of BK Ca channel, significantly blocked the berberine-induced…”

Section: Acute Application Of Berberine Directly Induced the Relaxatimentioning

confidence: 97%

“…Male Sprague-Dawley rats (~190 g) were purchased from Medical Laboratory Animal Center of Xi'an Jiaotong University. According to our previous description (Ma et al 2016), a high-fat diet plus low-dose injections of streptozotocin (STZ) were used to establish the diabetic rat. The high-fat diet consisted of 70% standard laboratory chow, 5% yolk powder, 10% lard and 15% carbohydrate.…”

Section: Animal Modelmentioning

confidence: 99%

“…Recently, human and animal studies indicated that berberine also had a hypoglycemic activity (Zhang & Chen 2012) and an extra-beneficial protection for cardiovascular system in hypertension or diabetes (Wang et al 2012, Li et al 2014, Ni et al 2015. Our previous work observed that berberine alleviated cerebral arterial contractility in streptozotocin (STZ)-induced diabetic rats by regulating the intracellular Ca 2+ handling of smooth muscle cells (Ma et al 2016). In addition, berberine has been reported to improve vascular endothelial function in humans (Cheng et al 2013) and show the protective effects for hypertensive patients with type 2 DM as the adjuvant therapy (Dai et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Berberine reduced blood pressure and improved vasodilation in diabetic rats

Liang

Zhang

et al. 2017

Journal of Molecular Endocrinology

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Hyperglycemia and hypertension are considered to be the two leading risk factors for vascular disease in diabetic patients. However, few pharmacologic agents could provide a combinational therapy for controlling hyperglycemia and hypertension at the same time in diabetes. The objectives of this study are to investigate whether berberine treatment could directly reduce blood pressure and identify the molecular mechanism underlying the vascular protection of berberine in diabetic rats. Berberine was intragastrically administered with different dosages of 50, 100 and 200 mg/kg/day to diabetic rats for 8 weeks since the injection of streptozotocin. The endothelium-dependent/-independent relaxation in middle cerebral arteries was investigated. The activity of large-conductance Ca-activated K channel (BK) was investigated by recording whole-cell currents, analyzing single-channel activities and assessing the expressions of α- and β1-subunit at protein or mRNA levels. Results of the study suggest that chronic administration of 100 mg/kg/day berberine not only lowered blood glucose but also reduced blood pressure and improved vasodilation in diabetic rats. Furthermore, berberine markedly increased the function and expression of BK β1-subunit in cerebral vascular smooth muscle cells (VSMCs) isolated from diabetic rats or when exposed to hyperglycemia condition. The present study provided initial evidences that berberine reduced blood pressure and improved vasodilation in diabetic rats by activation of BK channel in VSMCs, which suggested that berberine might provide a combinational therapy for controlling hyperglycemia and blood pressure in diabetes. Furthermore, our work indicated that activation of BK channel might be the underlying mechanism responsible for the vascular protection of berberine in diabetes.

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Section: Physical Characteristics Of Experimental Animalssupporting

confidence: 92%

Section: Acute Application Of Berberine Directly Induced the Relaxatimentioning

confidence: 76%

Section: Acute Application Of Berberine Directly Induced the Relaxatimentioning

confidence: 97%

Section: Animal Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Berberine reduced blood pressure and improved vasodilation in diabetic rats

Liang

Zhang

et al. 2017

Journal of Molecular Endocrinology

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Biologically active isoquinoline alkaloids covering 2014–2018

Shang

Yang

Morris‐Natschke

et al. 2020

Medicinal Research Reviews

146

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Isoquinoline alkaloids, an important class of N-based heterocyclic compounds, have attracted considerable attention from researchers worldwide since the early 19th century. Over the past 200 years, many compounds from this class were isolated, and most of them and their analogs possess various bioactivities. In this review, we survey the updated literature on bioactive alkaloids and highlight research achievements of this alkaloid class during the period of 2014-2018. We reviewed over 400 molecules with a broad range of bioactivities, including antitumor, antidiabetic and its complications, antibacterial, antifungal, antiviral, antiparasitic, insecticidal, anti-inflammatory, antioxidant, neuroprotective, and other activities. This review should provide new indications or directions for the discovery of new and better drugs from the original naturally occurring isoquinoline alkaloids.

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Salidroside improved cerebrovascular vasodilation in streptozotocin-induced diabetic rats through restoring the function of BKCa channel in smooth muscle cells

Wang

Zhang

et al. 2017

Cell Tissue Res

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Vessel disease is a kind of severe complication in diabetic patients. However, few pharmacologic agents can directly recover diabetic vascular function. Salidroside (SAL), a major ingredient from Rhodiola rosea, has been found to have an obvious hypoglycemic effect and a beneficial protection on vascular function in diabetes. However, whether SAL is a suitable treatment for diabetes has not so far been evaluated and the underlying mechanisms remain unknown. The present work aims to (1) investigate the potential effects of SAL on cerebrovascular relaxation in streptozotocin-induced diabetic rats or when exposed to acute hyperglycemia condition and (2) examine whether function of the BK channel is involved in SAL treatment for diabetic vascular relaxation. Our results indicate that chronic administration of 100 mg/kg/day SAL not only improves cerebrovascular relaxation but also increases BK β1-subunit expressions at both protein and mRNA levels and enhances BK whole-cell and single-channel activities in cerebral VSMCs of diabetic rats. Correspondingly, acute application of 100 μM SAL induces cerebrovascular relaxation by activation of the BK channel. Furthermore, SAL activated the BK channel mainly through acting on the β1-subunit in HEK293 cells transfected with hSloα+β1 constructs. We concluded that SAL improved vasodilation in diabetic rats through restoring the function of the BK-β1 subunit in cerebrovascular smooth muscle cells, which may be the underlying mechanism responsible for the vascular protection of SAL in diabetes.

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Berberine alleviates the cerebrovascular contractility in streptozotocin-induced diabetic rats through modulation of intracellular Ca2+ handling in smooth muscle cells

Cited by 31 publications

References 32 publications

Berberine reduced blood pressure and improved vasodilation in diabetic rats

Berberine reduced blood pressure and improved vasodilation in diabetic rats

Biologically active isoquinoline alkaloids covering 2014–2018

Salidroside improved cerebrovascular vasodilation in streptozotocin-induced diabetic rats through restoring the function of BKCa channel in smooth muscle cells

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