Berberine inhibits intestinal secretory response of Vibrio cholerae and Escherichia coli enterotoxins

Sack, Ragna; Froehlich, J L

doi:10.1128/iai.35.2.471-475.1982

Cited by 130 publications

(39 citation statements)

References 17 publications

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“…Traditional use of berberine‐containing plants centered on restoring normal intestinal health especially those compromised by Vibrio cholerae and Escherichia coli . The possible mechanism(s) of berberine action in this indication, as well as for other pharmacologic effects and its other applications, have been consistently studied in recent years 13. This underscores the importance of berberine as a potential nutraceutical.…”

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confidence: 91%

Matrix‐free thin‐layer chromatography/laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids

Shariatgorji

Spáčil

Maddalo

et al. 2009

Rapid Comm Mass Spectrometry

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Quaternary protoberberine alkaloids belong to a pharmaceutically important class of isoquinoline alkaloids associated with bactericidal, fungicidal, insecticidal and antiviral activities. As traditional medicine gains wider acceptance, quick and robust analytical methods for the screening and analysis of plants containing these compounds attract considerable interest. Thin-layer chromatography (TLC) combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful technique but suffers from dilution of the TLC bands resulting in decreased sensitivity and masking of signals in the low-mass region both due to addition of matrix. This study integrates for the first time conventional silica gel TLC and laser desorption ionization mass spectrometry (LDI-MS) thus eliminating the need for any external matrix. Successful separation of berberine (R(f) = 0.56) and palmatine (R(f) = 0.46) from Berberis barandana including their identification by MS are demonstrated. Furthermore, a robust electrospray ionization (ESI)-MS method utilizing residual sample from TLC for quantification of berberine applying selected reaction monitoring and standard addition method is presented. The amount of berberine in the plant root prepared for the study was determined to be 0.70% (w/w).

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confidence: 91%

Matrix‐free thin‐layer chromatography/laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids

Shariatgorji

Spáčil

Maddalo

et al. 2009

Rapid Comm Mass Spectrometry

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“…Berberine might not allow the formation of active and intact cholera toxins (Modak, Modak & Venkatraman 1970). Berberine also markedly inhibited the secretory response of E. coli heat‐stable enterotoxin in the infant mouse model (Sack & Froehlich 1982). Berberine sulphate has some effects to eliminate the plasmids of E. coli (Li, Wang & Hu 1994).…”

Section: Discussionmentioning

confidence: 99%

In vitroantibacterial effect of berberine hydrochloride and enrofloxacin to fish pathogenic bacteria

Zhang

Xie

et al. 2009

Aquaculture Research

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Berberine the main antibacterial substance in the rhizome of Coptis chinensis Franch, is used as an antimicrobial agent for a long time. This study was carried out to investigate the potential of berberine alone or in combination with enro£oxacin against six common ¢sh pathogens for use in ¢sh disease management in aquaculture. The minimum inhibitory concentrations (MICs) of berberine hydrochloride against Aeromonas hydrophila, Pseudomonas £uorescens, Vibrio vulni¢cus, Edwardsiella ictaluri, Escherichia coli and Streptococcus agalactiae were determined and to be 4500, 4500, 4500, 300, 400 and 100 mg mL À 1 respectively. The minimal bactericidal concentrations (MBCs) of berberine hydrochloride to E. coli, E. ictaluri and S. agalactiae were 300^500 mg mL À 1. In combination with enro£oxacin, the MICs and MBCs of berberine hydrochloride signi¢cantly decreased against E. coli and E. ictaluri but not against Streptococcus dysgalactiae. These results demonstrated that the berberine hydrochloride enhanced the bactericidal e¡ect of enro£oxacin and vice versa. The synergistic bactericidal e¡ect of berberine hydrochloride and enro£oxacin suggest its potential use in ¢sh disease management in aquaculture. This is the ¢rst study on the inhibitory e¡ect of berberine hydrochloride against ¢sh pathogenic bacteria.

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“…19 In addition, berberine inhibits the production of intestinal secretions induced by cholera toxin in vivo. [20][21][22] At a concentration of 10 mg/ml, berberine reduced by 70% intestinal secretion stimulated by the heatlabile toxin of Escherichia coli in the rabbit-ligated intestinal-loop model and inhibited the secretory response of the heat-stable toxin of E. coli in mice. 19,23 The suggested mechanism by which berberine exerts its antidiarrheal activity is through the activation of ␣ 2 -adrenoceptors and by reducing cyclic adenosine monophoshate production through the inhibition adenylate cyclase activity, which, in turn, decreases intestinal motility.…”

Section: Pharmacology and Mechanism Of Actionmentioning

confidence: 99%

Goldenseal (Hydrastis canadensis): Is There Enough Scientific Evidence to Support Safety and Efficacy?

Mahady

Chadwick²

2001

Nutrition in Clinical Care

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Over the past 10 years, botanical dietary supplements containing goldenseal (Hydrastis canadensis) have become some of the best‐selling products on the American market. Traditionally used by Native Americans to treat digestive complaints, goldenseal is now recommended for the treatment of common cold, influenza, menstrual disorders, and traveler's diarrhea, as well as muscular pain. In spite of these claims, there is little scientific evidence to suggest that goldenseal is effective for the prevention or treatment of any ailment. While some published data suggest that berberine, one of the active chemical constituents of goldenseal, is of some benefit for the treatment of bacterially induced diarrhea, it is not as effective as some standard antibiotics. Furthermore, considering the low concentration of berberine in goldenseal extracts and the lack of quality control over botanical dietary supplements in general, the concentration of berberine in goldenseal extracts is too low to be therapeutically effective. The safety of extended use of goldenseal extracts is also questionable, as another chemical constituent of the plant, hydrastine, may cause hypertension, exaggerated reflexes, and respiratory failure if given in sufficient doses. Furthermore, extracts of goldenseal inhibit the activity of cytochrome P450 (CYP 3A4) in vitro, and, therefore, may increase the toxicity of concomitantly administered prescription drugs t metabolized by this enzyme. Thus, due to a lack of substantiating scientific and clinical data for either safety or efficacy, the use of goldenseal is not recommended for any therapeutic indication.

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Berberine inhibits intestinal secretory response of Vibrio cholerae and Escherichia coli enterotoxins

Cited by 130 publications

References 17 publications

Matrix‐free thin‐layer chromatography/laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids

Matrix‐free thin‐layer chromatography/laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids

In vitroantibacterial effect of berberine hydrochloride and enrofloxacin to fish pathogenic bacteria

Goldenseal (Hydrastis canadensis): Is There Enough Scientific Evidence to Support Safety and Efficacy?

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