Berberine regulates the microRNA‑21‑ITGΒ4‑PDCD4 axis and inhibits colon cancer viability

Abstract

Berberine is sourced from multiple medicinal herb resources and is easy to extract. With the advantages of low price, safety and convenience, berberine may have potential for wide clinical use. The present study aimed to investigate whether berberine inhibited the viability of colon cancer and whether it regulated the three-gene network microRNA (miR)-21-integrin β4 (ITGβ4)-programmed cell death 4 (PDCD4). It was demonstrated that berberine treatment suppressed colon cancer cell viability, and induced apoptosis and activated caspase-3 activity in the human colon carcinoma HCT116 cell line. Berberine inhibited miR-21 expression and promoted ITGβ4 and PDCD4 protein expression in the HCT116 cell line. Overexpression of miR-21 reduced the anti-cancer effects of berberine on cell viability, apoptosis rate and caspase-3 activity of the HCT116 cell line. However, it was revealed that the overexpression of miR-21 also suppressed ITGβ4 and PDCD4 protein expression in the HCT116 cell line. In conclusion, miR-21, ITGβ4 and PDCD4 are involved in the anti-cancer effects of berberine on cell viability and apoptosis in the HCT116 cell line.

<p>Aberrant Expression Of PDCD4/eIF4A1 Signal Predicts Postoperative Recurrence For Early-Stage Oral Squamous Cell Carcinoma</p>

“…PDCD4 is downregulated or lost in certain aggressive human carcinomas including the breast cancer, lung and colon cancer,14–16 and it is speculated to shuttle between nucleus and cytoplasm of tumor cells 17. eIF4A1 has not been widely investigated in tumors, while its upregulation in cytoplasm of ER-negative breast cancer cells was associated with a poor prognosis 8.…”

Anticancer Potential of Natural Isoquinoline Alkaloid Berberine

“…62 Similarly, 1, 10, and 100 µM berberine depleted cell viability, increased levels of apoptosis, activated caspase-3, integrin β4 (ITGβ4), and programmed cell death 4 (PDCD4) protein expression, and inhibited miR-21 mRNA expression in HCT116 cells (Table 1). 63 Berberine (6.25, 12.5, 25, and 50 µM) concentration-dependently reduced cell proliferation and colony formation and arrested DLD-1 and Caco-2 cells in the G 0 /G 1 phase of the cell cycle and also depleted the S-phase fraction. Berberine also decreased glucose-induced lipogenesis in these cells and inhibited the mRNA expression of acetyl-CoA carboxylase (ACC), ATP citrate lyase (ACL), and fatty acid synthase (FASN), and decreased sterol regulatory element-binding protein-1 (SREBP-1) activation, SREBP cleavage-activating protein (SCAP) expression, and β-catenin signaling (Table 1).…”

Temperature and pH Dual Responsive Nanogels of Modified Sodium Alginate and NIPAM for Berberine Loading and Release

“…In the past two decades, there have been reports that berberine can induce apoptosis of solid tumor cancer cells. In the existing reports, berberine can inhibit the growth and proliferation of colon cancer, esophageal cancer, lung cancer, and liver cancer cells, 19 − 22 which shows its potential anticancer ability. However, berberine hydrochloride has low biocompatibility due to its poor water solubility, and a higher dosage is needed to achieve the desired effect.…”