Berberine treatment reduces atherosclerosis by mediating gut microbiota in apoE-/- mice

Shi, Yafei; Hu, Jiaxin; Geng, Jie; Hu, Tingting; Wang, Bingjian; Yan, Wei; Jiang, Yicheng; Li, Jiangjin; Liu, Shaowen

doi:10.1016/j.biopha.2018.08.148

Cited by 90 publications

(95 citation statements)

References 35 publications

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“…Alpha diversity analysis did not reveal a significant decrease in fecal microbial diversity after BBR treatment, which is inconsistent with other research results Zhu et al, 2018). However, in our study beta diversity analysis showed that the community compositional structure was significantly different after treatment with the high or low dose of BBR, which do support the findings of a previous study (Shi et al, 2018). Presently, Firmicutes (75.4%) and Bacteroidetes (15.1%) are the two most dominant phylum at the phylum level, accounting for 90.5%.…”

Section: Discussioncontrasting

confidence: 99%

“…This was similar to the composition of human gut microbiota in prior studies, with Bacteroidetes and Firmicutes constituting more than 90% of the taxa (Qin et al, 2010;The Human Microbiome Project Consortium, 2012). In our study, the higher abundance was followed by Proteobacteria (3.3%) and Actinobacteria (2.5%), which is inconsistent with the prior finding of Proteobacteria and Verrucomicrobia (Shi et al, 2018). In addition, only decreased Proteobacteria abundance in High group and increased Actinobacteria abundance in Low group was significantly observed after BBR treatment compared with the Control group.…”

Section: Discussioncontrasting

confidence: 99%

“…The anti-inflammatory effects of BBR, such as suppressing the expression of pro-inflammatory genes and improving HFDinduced systemic inflammation, have been widely reported (Jeong et al, 2009;Zhang et al, 2012). Previous studies in HFDinduced atherosclerosis mice reported that BBR administration remarkably reduced the mRNA expression of pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, in ileal and carotid arteries (Shi et al, 2018;Zhu et al, 2018). Consistent with these results, serum levels of TNF-α, IL-1β, and IL-6 were significantly decreased by the high dose of BBR, while in the low dose group, only TNF-α and IL-1β levels were decreased.…”

Section: Discussionmentioning

confidence: 99%

“…Heart tissues containing the aortic arch were excised from the proximal aortic root to the iliac artery branch and placed in a 4% paraformaldehyde environment for 6 h. The atherosclerotic lesion area of aorta was evaluated by en face Oil red O staining as previously described (Shi et al, 2018). Measurement of the lesion size was based on hematoxylin and eosin staining of sections of paraffin embedded aortic root.…”

Section: Plaque Lesion Analysismentioning

confidence: 99%

“…Previous studies have indicated that the anti-atherosclerotic effect of BBR may be associated with changes in the gut microbiota, specifically the increased abundance of Akkermansia, altered abundance of Firmicutes and Verrucomicrobia, and decreased serum trimethylamine N-oxide (TMAO) levels (Zhu et al, 2018;Shi et al, 2018). However, the specific linking of the role of BBR in gut microbiota with atherosclerosis and the potential mechanism are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE−/− Mice

et al. 2020

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Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota. Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated. The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE −/− mice fed a high-fat diet. A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious. Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/lowdensity lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR. Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR. 16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota. Specifically, BBR enriched the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter, and changed the abundance of Bilophila. These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism. Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses. Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment. The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE −/− mice. The effects are more pronounced for the high dose. This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid. Notably, gut microbiota alterations showed different sensitivity to BBR dose.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plaque Lesion Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE−/− Mice

et al. 2020

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Cardiovascular risk of dietary trimethylamine oxide precursors and the therapeutic potential of resveratrol and its derivatives

Hou,

Chen,

Hazeena

et al. 2024

FEBS Open Bio

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Overall diet, lifestyle choices, genetic predisposition, and other underlying health conditions may contribute to higher trimethylamine N‐oxide (TMAO) levels and increased cardiovascular risk. This review explores the potential therapeutic ability of RSV to protect against cardiovascular diseases (CVD) and affect TMAO levels. This review considers recent studies on the association of TMAO with CVD. It also examines the sources, mechanisms, and metabolism of TMAO along with TMAO‐induced cardiovascular events. Plant polyphenolic compounds, including resveratrol (RSV), and their cardioprotective mechanism of regulating TMAO levels and modifying gut microbiota are also discussed here. RSV's salient features and bioactive properties in reducing CVD have been evaluated. The close relationship between TMAO and CVD is clearly understood from currently available data, making it a potent biomarker for CVD. Precise investigation, including clinical trials, must be performed to understand RSV's mechanism, dose, effects, and derivatives as a cardioprotectant agent.

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Bioactive dietary components—Anti‐obesity effects related to energy metabolism and inflammation

et al. 2022

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Obesity is the result of the long-term energy imbalance between the excess calories consumed and the few calories expended. Reducing the intake of energy dense foods (fats, sugars), and strategies such as fasting and caloric restriction can promote body weight loss. Not only energy in terms of calories, but also the specific composition of the diet can affect the way the food is absorbed and how its energy is stored, used or dissipated. Recent research has shown that bioactive components of food, such as polyphenols and vitamins, can influence obesity and its pathologic complications such as insulin resistance, inflammation and

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Berberine treatment reduces atherosclerosis by mediating gut microbiota in apoE-/- mice

Cited by 90 publications

References 35 publications

Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE−/− Mice

Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE−/− Mice

Cardiovascular risk of dietary trimethylamine oxide precursors and the therapeutic potential of resveratrol and its derivatives

Bioactive dietary components—Anti‐obesity effects related to energy metabolism and inflammation

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