Bestimmung der biologischen Aktivit�t von synthetischem Secretin

Lehnert, Peter; Stahlheber, H.; Röder, Olaf; Zoelch, M.; Forell, M. M.; Wünsch, Erich; Jaeger, Ernst; Martens, HenriJ .

doi:10.1007/bf01484145

Cited by 16 publications

(7 citation statements)

References 13 publications

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“…After a basal period of 20 minutes, synthetic (Lehnert et al, 1973). The reproducibility of the effect of secretin infusions on pancreatic juice flow in the same subjects has been established (Tympner et al, 1974).…”

Section: Methodsmentioning

confidence: 99%

Effect of secretin on plasma motilin in man.

Mitznegg¹,

Bloom²,

Domschke³

et al. 1977

Gut

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SUMMARY Graded doses of 0 1, 0*3, 0 9, and 2-7 clinical units/kg/h of pure synthetic secretin were infused over 60 minute periods in six healthy volunteers. Duodenal bicarbonate output and pH were recorded and plasma secretin and motilin levels were measured by radioimmunoassay. During the infusions plasma motilin fell in a dose dependent manner to a nadir of 35 %. This fall was linearly correlated with pancreatic bicarbonate output, whereas a non-linear correlation was observed between plasma motilin and both plasma secretin level and duodenal pH. It is suggested that plasma motilin levels are decreased by secretin-induced pancreatic bicarbonate juice flow. This may be important for the control of motilin secretion initiated by duodenal acidification and the concomitant delay in gastric emptying.Motilin, a recently discovered hormonal peptide from the small intestine (Brown et al., 1971), has been shown to increase lower oesophageal sphincter pressure and to delay gastric emptying in man (Ruppin et al., 1975; Rosch et al., 1976).So far, no physiological regulation of motilin secretion has been ascertained. In the dog it has been reported that serum motilin levels are raised by strong duodenal alkalinisation (Dryburgh and Brown, 1975). We have shown recently that, in man, motilin is released by duodenal acidification and after intraduodenal instillation of tris buffer (pH 102) motilin fell . As, however, a duodenal pH of 10-2 is unlikely to be achieved under normal conditions, the significance of this finding is uncertain. The present study was designed to assess the effect on motilin release of secretin. This peptide initiates a physiological alkalinisation of the duodenum by stimulating endogenous alkaline pancreatic juice flow (Jorpes and Mutt, 1961). MethodsSix healthy volunteer men (aged 23-42 years) were "Address for reprint requests:

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Section: Methodsmentioning

confidence: 99%

Effect of secretin on plasma motilin in man.

Mitznegg¹,

Bloom²,

Domschke³

et al. 1977

Gut

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“…The synthetic secretin used in these studies is supplied in microgram quantities. Because the dosage of secretin is generally expressed in units, and this forms the basis for comparison with other studies of this peptide, our infusion rates of secretin were calculated in units, using the conversion factor of 3-5 U/microgram obtained for this preparation of synthetic secretin against pure secretin (Mutt) and secretin with cysteine (GIH Research Laboratory) in studies of canine pancreatic secretion (Lehnert et al, 1973). PROTOCOL Each animal was fasted for 18 hours before study, and each study lasted for six hours.…”

Section: Experimental Substancesmentioning

confidence: 99%

Quantitative comparison of the effects of cholecystokinin, secretin, and pentagastrin on gastrointestinal myoelectric activity in the conscious fasted dog.

Wingate¹,

Pearce²,

Hutton³

et al. 1978

Gut

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SUMMARYThe effects on gastrointestinal myoelectric activity of infused pentagastrin, cholecystokinin (CCK), and secretin at physiological doses were studied in live dogs with implanted serosal electrodes during 56 six-hour studies. Pentagastrin dose-dependently increased gastric and duodenal slow-wave frequencies; secretin and CCK did not. Pentagastrin and CCK diminished the incidence of fasting migrating myoelectric complexes (MMCs), but MMCs were abolished only in the proximal small intestine. Pentagastrin infusion was not reflected in an increased number of spikes, whereas CCK induced a dose-dependent increase in jejunal spike activity. Secretin dose-dependently decreased duodenal and jejunal spike incidence without a marked effect on MMC incidence. Analysis of patterns of spike activity showed significant dose-dependent changes with all three peptides. The different effects of pentagastrin and CCK on spike activity in these studies may have been a consequence of pentagastrin-stimulated gastric acid secretion. None of the three peptides produced a pattern of myoelectric activity which closely resembled that seen on feeding; since, unlike food, all three peptides had little or no effect on the distal small intestine, it seems unlikely that combinations of these peptides are responsible for the change induced by food. The failure of these peptides to abolish fasting patterns in the distal intestine suggests a possible mechanism for some types of postvagotomy dysfunction.

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“…The methods used for determining the relative potency of the hormones were described earlier [21]. The volume and bicarbonate secretion was used as a parameter for the bio logical activity of secretin, and the protein and enzyme secretion for the biological activity of cholecystokinin.…”

Section: Methodsmentioning

confidence: 99%

Studies on the Elimination of Secretin and Cholecystokinin with Regard to the Kinetics of Exocrine Pancreatic Secretion

Lehnert¹,

Stahlheber²,

Forell³

et al. 1974

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Studies were performed on 72 anesthetized mongrel dogs. Upon injection or infusion of secretin into the portal vein, the pancreatic volume and bicarbonate response was found to be significantly smaller than following administration into the femoral vein, but only when low doses of secretin (0.25 clin U/kg/h or smaller) were applied. No difference was observed in pancreatic protein and enzyme secretion after cholecystokinin, when both high and low doses were applied to the portal or femoral vein. The injection of secretin and cholecystokinin into the renal artery causes a loss of biological activity of 75 and 60%, respectively, as compared to femoral administration. Ligature of the blood vessels of both kidneys resulted in an increase in the biological activity of secretin of 80% and of cholecystokinin of 50%. The slopes of the flow rate curves determined upon secretin injection were smaller after ligature of the renal blood vessels. The biological half-life of secretin increased from an average of 2.9 to 4.8 min. These results indicate that the kidneys play a role in the catabolism of exogenous secretin and cholecystokinin, whereas the blood and the liver (at least for cholecystokinin) seem to be of small importance.

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Bestimmung der biologischen Aktivit�t von synthetischem Secretin

Cited by 16 publications

References 13 publications

Effect of secretin on plasma motilin in man.

Effect of secretin on plasma motilin in man.

Quantitative comparison of the effects of cholecystokinin, secretin, and pentagastrin on gastrointestinal myoelectric activity in the conscious fasted dog.

Studies on the Elimination of Secretin and Cholecystokinin with Regard to the Kinetics of Exocrine Pancreatic Secretion

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