Bestimmung von deoxynivalenol mittels LC - MS/MS (Tandem Massenspektrometrie)

Abstract

A sensitive and selective method using high-performance liquid chromatography in combination with atmospheric pressure chemical ionization tandem mass spetrometry (LC-APCI-MS/MS) has been developed for the determination of Deoxynivalenol (DON) in trace levels. The extract was purified with a MultiSep™ column followed by the Vicam™ DON immunoaffinity column. Quantification is based on an external standard method using positive Multiple Reaction Monitoring (MRM). The limit of detection was 5 μg/kg with a signal to noise ratio of 3:1.

Highly deoxynivalenol contaminated oats and immune function in horses

“…Levels of different relevant trichothecenes in the oats were analysed at the Institute of Risk Assessment (BfR, Berlin, Germany) using an accelerated solvent extraction online clean-up and high pressure liquid chromatography coupled with tandem mass spectrometry as described previously (Kappenstein et al 2002). The recovery rate for oats was 99.6% at a DON-concentration of 1 ppm, the detection limit was 5.1 ppb and the quantification limit 15.5 ppb.…”