Beta 3-adrenoceptor in guinea pig brown and white adipocytes: low expression and lack of function

Atgié, Claude; Tavernier, Geneviève; Dallaire, François; Bengtsson, Tore; Marti, Luc; Carpéné, Christian; Lafontan, Max; Bukowiecki, L. J.; Langin, Dominique

doi:10.1152/ajpregu.1996.271.6.r1729

Cited by 15 publications

(14 citation statements)

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“…Head width, crown rump length, abdominal circumference, body weight/crown rump length, and ponderal index (body weight/crown rump length 3 ) were reduced in fetuses of feedrestricted mothers at day 60 of gestation (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…As in humans, adipose depots containing BAT in the guinea pig are transformed after the neonatal period into tissues with the characteristics of white adipose tissue (9,21). Cold acclimation can reactivate BAT in adult guinea pigs (3,21). Uncoupling protein-1 mRNA is detected in adipose tissue in adult humans, suggesting that small numbers of brown adipocytes are present (9,10,20,35).…”

Section: Discussionmentioning

confidence: 99%

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Chronic maternal feed restriction impairs growth but increases adiposity of the fetal guinea pig

Kind¹,

Roberts²,

Sohlström³

et al. 2005

American Journal of Physiology-Regulatory, Integrative and Comp

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Small size at birth has been associated with an increased risk of central obesity and reduced lean body mass in adult life. This study investigated the time of onset of prenatally induced obesity, which occurs after maternal feed restriction, in the guinea pig, a species that, like the human, develops substantial adipose tissue stores before birth. We examined the effect of maternal feed restriction [70% ad libitum intake from 4 wk before to midpregnancy, then 90% until day 60 gestation (term ∼69 days)] on fetal growth and body composition in the guinea pig. Maternal feed restriction reduced fetal (−39%) and placental (−30%) weight at 60 days gestation and reduced liver, biceps muscle, spleen, and thymus weights, relative to fetal weight, while relative weights of brain, lungs, and interscapular and retroperitoneal fat pads were increased. In the interscapular depot, maternal feed restriction decreased the volume density of multilocular fat and increased that of unilocular fat, resulting in an increased relative weight of interscapular unilocular fat. Maternal feed restriction did not alter the relative weight of perirenal fat or the volume density of adipocyte populations within the depot but increased unilocular lipid locule size. Maternal feed restriction in the guinea pig is associated with decreased weight of major organs, including liver and skeletal muscle, but increased adiposity of the fetus, with relative sparing of unilocular adipose tissue. If this early-onset obesity persists, it may contribute to the metabolic and cardiovascular dysfunction that these offspring of feed-restricted mothers develop as adults.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chronic maternal feed restriction impairs growth but increases adiposity of the fetal guinea pig

Kind¹,

Roberts²,

Sohlström³

et al. 2005

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Since human adipocytes are also devoid of functional β 3 -ARs (8,24,32), this can be the major reason for the low number of phase II or III developments of orally given β 3 -AR agonists for the indications of obesity or diabetes (29). Another resemblance between guinea pig and human adipocytes is that, besides their poor β 3 -adrenergic receptivity, both exhibit poor responsiveness to insulin, at least regarding the stimulation of glucose upake (4,23). This stricking association between low β 3 -adrenergic and low insulin responsiveness has been already noted in guinea pig brown adipocytes by Himms-Hagen and coworkers (20).…”

Section: Discussionmentioning

confidence: 99%

“…These controversial observations on the effects of β 3 -AR agonists on glucose utilisation prompted us to verify in two models whether sustained β 3 -adrenergic activation was modifying glucose transport capacity in adipocytes. The three β-AR subtypes, including the β 3 -receptor are present on rat adipocytes (16) while only β 1 -, and β 2 -ARs are functional in guinea pig adipocytes (4,8,10). Since we have reported that the β 3 -AR agonist CL 316243 induces adipose tissue remodeling in rat but not in guinea pig (14) we aimed to compare in rat and guinea pig adipocytes the influence of chronic CLtreatment on glucose transport activity and insulin responsiveness.…”

mentioning

confidence: 99%

Prolonged treatment with the β3-adrenergic agonist CL 316243 induces adipose tissue remodeling in rat but not in guinea pig: 2) modulation of glucose uptake and monoamine oxidase activity

Duffaut

Bour

Prévot

et al. 2006

J. Physiol. Biochem.

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Beta3-adrenergic agonists are well-recognited to promote lipid mobilisation and adipose tissue remodeling in rodents, leading to multilocular fat cells enriched in mitochondria. However, effects of beta3-adrenergic agonists on glucose transport are still controversial. In this work, we studied in white adipose tissue (WAT) the influence of sustained beta3-adrenergic stimulation on the glucose transport and on the mitochondrial monoamine oxidase (MAO) activity. As one-week administration of CL 316243 (CL, 1 mg/kg/d) induces beta-adrenergic desensitization in rat but not in guinea pig adipocytes, attention was paid to compare these models. When expressing glucose uptake as nmoles of 2-deoxyglucose/100 mg cell lipids, maximally stimulated uptake was increased in adipocytes of WAT from treated rats but not from treated guinea pigs. However, basal hexose uptake was also increased in CL-treated rats and, as a consequence, the dose-dependent curves for insulin stimulation were similar in control and CL-treated rats when expressed as fold increase over basal. Insulin-induced lipogenesis was unchanged in rat or guinea pig adipocytes after CL-treatment. The glucose carriers GLUT4 and corresponding mRNA were increased in subcutaneous WAT or in brown adipose tissue (BAT) but not in visceral WAT or muscles of CL-treated rats. There was an increase of MAO activity in WAT and BAT, but not in liver, of CL-treated rats while no change was detected in guinea pigs. These findings show that only rat adipocytes, which are beta3-adrenergic-responsive, respond to chronic beta3-AR agonist by an increase of GLUT4 content and MAO activity, despite a desensitization of all beta-adrenoceptor subtypes.

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“…However, white fat cells from primates -including humans -exhibit only weak activation of lipolysis in response to β 3 -AR agonists while they respond to β 1 -or β 2 -AR agonists (Langin et al 1991;Bousquet-Mélou et al 1994;Tavernier et al 1996;Umekawa et al 1996). Guinea-pig adipocytes are also unresponsive to β 3 -AR stimulation Atgié et al 1996) while dog fat cells share equivalent β 1 -, β 2 -or β 3 -adrenergic responsiveness (Galitzky et al 1993b;1995). We have reported already that octopamine stimulates lipolysis in canine adipocytes in a manner resistant to the blockade by β 1 -or β 2 -selective antagonists, suggesting activation of atypical β-ARs (Galitzky et al 1993a).…”

Section: Introductionmentioning

confidence: 99%

Selective activation of β3-adrenoceptors by octopamine: comparative studies in mammalian fat cells

Carpéné¹,

Galitzky

Fontana³

et al. 1999

Naunyn-Schmiedeberg's Arch Pharmacol

128

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Numerous synthetic agonists selectively stimulate beta3-adrenoceptors (ARs). The endogenous catecholamines, noradrenaline and adrenaline, however, stimulate all the beta-AR subtypes, and no selective physiological agonist for beta3-ARs has been described so far. The aim of this study was to investigate whether any naturally occurring amine can stimulate selectively beta3-ARs. Since activation of lipolysis is a well-known beta-adrenergic function, the efficacy and potency of various biogenic amines were compared with those of noradrenaline, isoprenaline, and beta3-AR agonists 4-(-{[2-hydroxy-(3-chlorophenyl)ethyl]-amino} propyl)phenoxyacetate (BRL 37,344) and (R,R)-5-(2-{[2-(3-chlorophenyl )-2-hydroxyethyl]-amino} propyl)-1,3-benzo-dioxole-2,2-dicarboxylate (CL 316,243) by testing their lipolytic action in white fat cells. Five mammalian species were studied: rat, hamster and dog, in which selective beta-AR agonists act as full lipolytic agents, and guinea-pigs and humans, in which beta3-AR agonists are less potent activators of lipolysis. Several biogenic amines were inefficient (e.g. dopamine, tyramine and beta-phenylethylamine) while others (synephrine, phenylethanolamine, epinine) were partially active in stimulating lipolysis in all species studied. Their actions were inhibited by all the beta-AR antagonists tested, including those selective for beta1- or beta2-ARs. Octopamine was the only amine fully stimulating lipolysis in rat, hamster and dog fat cells, while inefficient in guinea-pig or human fat cells, like the beta3-AR agonists. In rat white fat cells, beta-AR antagonists inhibited the lipolytic effect of octopamine with a relative order of potency very similar to that observed against CL 316,243. Competitive antagonism of octopamine effect resulted in the following apparent pA2 [-log(IC50), where IC50 is the antagonist concentration eliciting half-maximal inhibition] values: 7.77 (bupranolol), 6.48 [3-(2-ethyl-phenoxy)-1[(1 S)-1,2,3,4-tetrahydronaphth-1-ylaminol]-(2S)2-propanol oxalate, SR 59230A, a beta3-selective antagonist], 6.30[erythro-D,L-1(7-lethylindan-4-yloxy)-3-isopropylamino-+ ++butan-2-ol, ICI 118,551, a beta2-selective antagonist] and 4.71 [(+/-)-[2-(3-carbomyl-4-hydroxyphenoxy)-ethylamino]-3-[4-(1- methyl-4-trifluoromethyl-2-imidazolyl)-phenoxy]2-propanolmethane sulphonate, CGP 20712A, a beta1-selective antagonist]. Octopamine had other properties in common with beta3-AR agonists: stimulation of oxygen consumption in rat brown fat cells and very low affinity in displacing [3H]CGP 12,177 binding to [beta1- or beta2-ARs in dog and rat adipocyte membranes. In Chinese hamster ovary (CHO) cells expressing human beta3-ARs, octopamine inhibited [125I]ICYP binding with only twofold less affinity than noradrenaline while it exhibited an affinity around 200-fold lower than noradrenaline in CHO cells expressing human beta1- or beta2-ARs. These data suggest that, among the biogenic amines metabolically related to catecholamines, octopamine can be considered as the most selective for beta3-ARs.

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Beta 3-adrenoceptor in guinea pig brown and white adipocytes: low expression and lack of function

Cited by 15 publications

References 0 publications

Chronic maternal feed restriction impairs growth but increases adiposity of the fetal guinea pig

Chronic maternal feed restriction impairs growth but increases adiposity of the fetal guinea pig

Prolonged treatment with the β3-adrenergic agonist CL 316243 induces adipose tissue remodeling in rat but not in guinea pig: 2) modulation of glucose uptake and monoamine oxidase activity

Selective activation of β3-adrenoceptors by octopamine: comparative studies in mammalian fat cells

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